教育制度监督并重预防国土资源系统腐败案件的发生——读《国土资源系统警示教育案例》一书有感

日前。省国土资源厅下发了由中央纪委驻国土资源部纪检组、监察部驻国土资源部监察局组织编写的《国土资源系统警示教育案例》一书,书中列举了发生在我们国土资源系统的50个典型违纪违法案例,并进行了剖析,提出了“警示与戒鉴”的意见;特别是徐绍史部长在书中开篇的题词“要大喝一声、猛击一掌,进一步警醒起来,通过两到三年的努力基本遏制国土资源系统腐败案件易发多发的势头,力争使违法违纪案件数量降下来,行风评议的名次升上去”。这再一次对我们国土资源系统及每一位党员干部和职工,敲响了廉政警钟,提出了廉政要求。     

充分发挥“五个力”的作用 促进党风廉政建设再上新台阶

近几年来,国土系统始终坚持把反对和有效预防腐败作为重要的政治任务和"生命线"工程,紧紧依靠广大党员干部,求真务实、开拓创新,不断开创党风廉政建设和反腐败工作新局面。但同时,我们也看到,由于国土系统资源相对集聚、权力相对集中、资金流量相对     

中华鳖(Pelodiscus sinensis)摩氏摩根菌(Morganella morganii)的鉴定及致病性研究

从3只患有头面部疖疮中华鳖(Pelodiscus sinensis)的肝脏、肾脏、头面部病灶干酪样组织,分离得到12株细菌。综合菌落形态观察、16S rRNA序列分析与生化特性分析方法,所有分离株均为摩氏摩根菌(Morganella morganii)。稚鳖和小鼠感染试验提示该菌具有较强的致病力,其对小鼠的半数致死量(LD50)为107.24。同时采用常规琼脂扩散(K-B)法进行8类14种常用药物的抗菌敏感性测定,结果表明,分离株对氨基糖苷类、β-内酰胺类、四环素类、林可酰胺类、多肽类、喹诺酮类、磺胺类等中的8种药物耐药,对氨曲南、氟苯尼考、头孢哌酮则较为敏感。本研究为中华鳖以头面部疖疮为主要临床特征的摩氏摩根菌病的首例报道,旨在为该病的确诊与防治提供科学依据和参考。     

曼氏无针乌贼(Sepiella maindroni)sigma-型谷胱甘肽硫转移酶基因的克隆及重组表达

采用EST结合RACE技术进行了曼氏无针乌贼(Sepiella maindroni)sigma-型GST(SmGST)基因的克隆。结果表明,该基因的cDNA全长为838bp,其中5′非编码区为73bp,3′非编码区为150bp,开放阅读框为615bp,编码的蛋白含有204个氨基酸。该基因的氨基酸序列中包含1个典型的GST-N结构域和一个GST-C结构域,与栉孔扇贝和长牡蛎的sigma-型GST的相似度分别为40.3%和39.3%。将该基因的编码区重组到pET-21(a+)载体后在大肠杆菌中得到诱导表达。重组SmGST的GST活力为(12.22±0.92)U/mg蛋白。     

宁波沿海陆源排污口假单胞菌属(Pseudomonas)分布特点

采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析, 得到2011年3月、5月、8月、10月份各排污口假单胞菌属的分布情况。结果表明, 宁波沿海陆源排污口中存在较多的假单胞菌属。在检出的假单胞菌属中, 维罗纳假单胞菌(Pseudomonas veronii)和莓实假单胞菌(Pseudomonas fragi)的含量相对较多, 分别占56.72%、13.904%; 排污口中假单胞菌属的数量存在季节性差异, 3月份、5月份假单胞菌的数量相对较多, 8月份和10月份较少, 推测与季节性温度变化有关; 假单胞菌属的含量与排污口的主要污染物有关, 氨氮含量较高的排污口假单胞菌属的含量更高。     

Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria

Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 ± 0.08, compared with 0.41 ± 0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.     

Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi.     

佩氏拟多甲藻及其硅藻共生体的分析

佩氏拟多甲藻Peridiniopsis penardii(Lemmermann)Bourrelly是1种淡水甲藻,可以形成水华,分布在意大利,美国,日本和中国。在2009年春季,厦门九龙江北溪发生水华。分离出来的细胞在培养条件下形成休眠包囊,细胞经光镜,扫描电镜(SEM),以及核糖体rDNA 18S序列分析,证实引起水华的是佩氏拟多甲藻。包囊椭圆形,直径大约28μm。细胞上鞘呈圆锥形,下鞘圆形或圆锥形,长23～36μm,宽23～31μm。甲板方程式为Po,x,4’,0a,6’’,5c(或t+4c),5s,5’’’,2’’’’。佩氏拟多甲藻具有2个细胞核,其中一个为自身细胞核,另外一个为共生的硅藻细胞核。我们对佩氏拟多甲藻和共生硅藻的18s进行了测定并构建了基因树,他们的系统发育位置和形态学分类相吻合。     

辽河口湿地耐盐石油降解菌作用效果与条件优化研究

采用投加过氧化氢、吐温-80和活性炭纤维等方法对筛选、分离自辽河口湿地石油污染土壤样品的耐盐高效石油降解菌的优化条件进行研究。结果表明,过氧化氢的最佳投加量为150mg/L;吐温-80的最佳投加量为160mg/L;活性炭纤维的最佳投加量为6g/L。3种优化条件下石油降解率均有增加,培养1周后,耐盐菌株在10℃下的石油降解率在40%以上,而在最适温度30℃下的石油降解率达到80%以上。     

谢姓的祖居地在哪里？

谢姓在《百家姓》中排名第24位,约有人口936万,占全国人口总数的0．72％。 关于谢姓的先祖来源,许多姓氏书都说它出自炎帝,炎帝,又称神农氏,作为中华民族人文始祖之一,为世代所敬仰,也被谢姓奉为始祖之一,谢姓家族是姜姓部落的后裔。如《元和姓纂》卷九中说：“谢,姜姓,炎帝之胤。”