急性高温低氧胁迫下刺参体腔液细胞miRNA表达谱的构建和靶标基因的功能分析

为了探究刺参应对极端高温、低氧环境的内在分子调控机制,促进刺参养殖产业的可持续发展.本研究以刺参体腔液细胞作为研究对象,分别构建了高温、低氧下miRNA的差异表达谱并分析了差异表达miRNAs的靶基因功能.研究表明:高温组中共发掘了20个差异表达miRNA,其中11个上调,9个下调;低氧组中共发掘了10个差异表达miR...     

马氏珠母贝育珠细胞小片和珠核处理技术研究Ⅰ:不同处理液在海南陵水育珠的效果

研究8组不同小片和核处理液在海南陵水海区的育珠效果,优化、筛选适宜该海区育珠的小片处理液及与之匹配的珠核处理液。结果显示:1)处理组手术贝休养期的吐核率与死亡率均显著低于对照组,表明处理液可促进伤口愈合并抗感染;2)处理组育珠8个月后的珠层厚度平均可达0.5 mm以上,与对照组0.3 mm的珠层厚度差异极显著(P<0.01),表明处理液具有明显促进珍珠质分泌的作用;3)优化筛选出核处理液C3,其增强核亲和力的效果明显优于C1(P<0.05);4)W处理组育珠贝死亡率平均小于22%,明显低于对照组(36.7%)和Y处理组(31.45%)(P<0.01),表明W处理液效果优于Y处理液;5)在8个处理组中,筛选出W0、W2、Y3三个匹配组,其育珠效果明显优于对照组(P<0.01)。引入综合评价指数即优良珍珠数占育珠贝总数的比例,以量化育珠效果便于比较     

咽旁间隙内腮腺基底细胞腺瘤的CT诊断

目的:探讨咽旁间隙内腮腺基底细胞腺瘤(BCA)的CT特征及其与咽旁间隙的神经鞘瘤的鉴别要点。方法:报道并分析了咽旁间隙内基底细胞腺瘤1例的少见CT表现。复习了自2010年以来国内关于CT诊断腮腺基底细胞腺瘤的所有文献,其中有详尽数据和完整描述的,且每篇报道在10例以上的文献仅4篇,共计77例,83个病灶。本文对这些病灶的个数、大小、所处腮腺的部位、囊变及钙化进行了小计。结果:单发者占94.8%,位于腮腺浅叶者占86.7%,小于3 cm者占92.8%,囊变者占60.2.%,钙化者占2.4%;咽旁间隙内腮腺基底细胞腺瘤位于二腹肌后腹的浅面,咽旁间隙脂肪内移。结论:咽旁间隙腮腺基底细胞腺瘤的CT表现具有一定的特征性,二腹肌后腹是鉴别咽旁间隙内基底细胞腺瘤和神经鞘瘤的分界线。      

无周围胆管扩张的肝内肿块型胆管细胞癌CT表现

目的:探讨无周围胆管扩张的肿块型肝内胆管细胞癌(ICC)的CT表现及病理基础,以提高其CT诊断水平。方法:回顾性分析22例经病理证实为ICC且CT扫描未见周围胆管扩张患者的CT及病理资料。结果:22例均为腺癌,3例为混合型胆管细胞癌-肝细胞癌;以高、中分化癌多见(16/22);大体分型均为肿块型,具有肿块型ICC的CT及病理特征:不规则肿块(外缘浅分叶状)14例,类圆形8例;肿块内含有大量的纤维或者粘液成分(硬癌型15例,髓质型7例);钙化(3/22);包膜皱缩征(10/22);渐进性延迟强化(19/22),渐进性-快进快出混合型强化(3/22);与典型ICC不同表现:病灶周围无胆管扩张,未见卫星灶及淋巴结转移。结论:不伴有周围胆管扩张ICC病理以高、中分化为主,侵袭扩散相对少见,具有肿块型ICC的影像特征,但无特征性周围胆管扩张,易误诊为不典型肝癌或感染性病变。      

不典型少突胶质细胞肿瘤的影像表现

目的:探讨不典型少突胶质细胞肿瘤(OG)的影像表现,提高诊断水平。方法:回顾分析12例经病理证实不典型OG的临床CT和MRI资料,探讨其影像特征。结果:年龄范围:23~60岁,平均41.5岁,其中40岁以上10例(83.3%)。7例表浅,5例深在,仅1例多发,共13个病灶。9例呈实性,MRI平扫呈稍长T1稍长T2信号,边缘欠清,其中1例信号均匀,增强后未见强化,8例信号不均,内见坏死、出血,增强后,7例呈斑片状轻-中度强化,1例呈"花环状"强化,且DWI明显弥散受限。3例呈完全囊性,囊液呈长T1长T2信号,壁较厚,呈等T1稍长T2信号,边缘不清,增强后均未见强化,1例DWI序列囊壁不均匀弥散受限。3例CT片上可见钙化,2例瘤周见大片水肿,2例跨中线,1例向深部浸润、侵犯邻近基底节区及丘脑。结论:不典型OG包括:中老年人、多发、位置深在、瘤周大片水肿、跨中线生长、弥漫深部浸润、增强后环形强化、DWI明显弥散受限、完全囊性,其影像表现仍具有一定特征,综合分析可提高诊断准确率。      

非均匀断层结构地震活动细胞自动机模拟系统几个算法设计

为研究介质非均匀性与地震活动的关系,编制开发了模型可定制的二维细胞自动机模拟软件。针对软件设计中出现的非均匀断层结构模型的建立、系统模拟数据记录结构设计以及内存管理等问题,提出了解决方案。改善了模拟系统所需的几种关键算法,使研究人员能根据需要灵活设定模型的介质参数。并利用内存管理技术,在一定程度上解决了大型模拟系统中普遍存在的硬件瓶颈问题,从而提高了细胞自动机模拟系统的适应性。     

Histological study on the skin of Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

Histological development of Japanese flounder Paralichthys olivaceus larval skin and ultrastructural difference of skin between reared normal and malpigmented Japanese flounder were studied with light microscopy (LM) and transmission electron microscopy (TEM). The results show that the skin develops slowly before the metamorphosis, while at the onset of metamorphosis, the skin develops quickly and becomes complete in structure till about 50d after being hatched. Ultrastructural observation on the normal and malpigmented skins shows that the iridophore and melanophore are adjacent to each other. Profile and structure of the two kinds of pigmcnt cells are more complete in the skin of normal ocular side than in the skin of pigmented blind side. The ultrastructure of typical chloride cell was observed in the skin of Japanese flounder larvae for the first time.     

Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species

1Introduction Japanese grenadier anchovy,Coilia ectenes Jordanet Seale(1905),is distributed in the northwest Pacif-ic.The congener,Osbeck's grenadier anchovy,Coil-ia mystus(Linnaeus,1758),is widely distributed inthe west Pacific.Both species inhabit coastal watersand estuaries and can reach up to rivers.The twospecies are important contributors to the coastal fish-eries of China(Zheng et al.,2003).Many studies on the fishery biology of the twospecies have been conducted(Zheng et al.,2003)…     

PROPERTIES OF PROTEOLYTIC TOXIN OF VIBRIO ANGUILLARUM FROM DISEASED FLOUNDER

Extracellular products(ECP)produced by Vibrio anguillarum strain M3 originally isolated from diseased flounder(Paralichthys olivaceus)were prepared.ECP of M3 showed gelatinase,casinase,amylase and haemolytic activity on agarose plates.High protease activity against azocasin was detected.Bacterium M3 showed highest growth and protease activity at 25℃。The Protease present in ECP showed maximal activity at pH 8 and 55℃；was completely inactivated by application of 80℃ heat for 30 min;was completely inhibited by EDTA and HgCl2,and was partially inhibited by PMSF,SDS,MnCl2 and iodoacetic acid;but not inhibited by CaCl2 and MgCl2.The ECP was toxic to flounder fish at LD50 values of 3.1 μg protein/g body weight.The addition of HgCl2 and application of heat at 50℃ decreased the lethal toxicity of ECP.When heated at 100℃,ECP lethality to flounder was completely in-hibited.After intramuscular injection of ECP into flounder,it showed evident histopathological changes including necrosis of muscle,extensive deposition of haemosiderin in the spleen,dilated bolld vessels congested with numerous lymphocytes in the liver.These results showed that ECP protease was a lethal factor produced by the baterium V.anguillarum M3.     

利用14C标记技术测定海洋初级生产力的绉议

<正>海洋初级生产力是指海洋中初级生产者(主要是浮游植物)通过光合作用或化学合成生产有机物的能力或速率[1-2],它是海洋生态系统中其他异养生物的生存基础,并从根本上影响着全球生物地球化学循环[3]与气候变化[4]。研究表明,海洋浮游植物通过光合作用不仅贡献了生物圈内的一半净初级生产力(以碳计约为5×1013 kg/a)[4],提供了生态系统中其他有机体生长与新陈代谢所需要的初级有机物能源,限制