2-[[(2-氨基苯基)亚胺]苯甲基]-4-氯苯酚晶体结构的量子化学研究

采用密度泛函理论(DFT)B3LYP方法,运用Gaussian03量子化学程序包,在B3LYP/6-31G*基组水平上对合成的化合物2-[[(2-氨基苯基)亚胺]苯甲基]-4-氯苯酚的晶体结构进行优化,计算了分子稳定构型的总能量、前线分子轨道能量、原子自然电荷布局、自然键轨道(NBO)及稳定化能等.计算结果与通过单晶X-射线衍射法测得的晶体结构的实验结果吻合.此项研究可为异双希夫碱配体及其金属配合物的合成及实际应用提供理论参考.     

A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.     

Non-specific immune response of bullfrog <Emphasis Type="Italic">Rana catesbeiana</Emphasis> to intraperitoneal injection of bacterium <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis>

Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally （i.p.） with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution （0.85%, w/v）. Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection （dpi） for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase （SOD） activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.     

五道沟金矿床控矿因素及找矿方向

一、成矿地质背景 五道沟金矿床位于黑龙江省东宁县境内,大地构造位置处于兴凯湖一布列亚山地块区张广才岭—太平岭边缘隆起带的南端。太平岭隆起带作为佳木斯地块南缘的增生部分,晚元古代张广才岭期运动形成褶皱隆起,其基底地层主要为上元古界黄松群变质岩及同期花岗岩,出露面积较大。黄松群地层变质程度自上而下由浅变深,岩性属滨海一浅海相碎屑沉积碳酸盐岩建造。张广才岭期沿北东向、北北东向断裂分布并伴有印支期碱长花岗岩、花岗斑岩的侵入,后期闪长岩脉侵入与金矿（化）体的形成关系密切,并控制其分布。     

Microsatellite Variation in the Oyster Crassostreaplicatula Along the Coast of China

Genetic diversity and differentiation of the oyster Crassostreaplicatula populations from China's coast were studied basedon seven microsatellite loci.All loci showed high polymorphism for all five C.plicatula populations,with an average number of alleleper locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952.Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles.Microsatellite analysis revealed significant subdivision in the C.plicatula populations.According to the neighbor-joining tree con-structed on the basis of the D,A distance,the five populations fell into three regional groups,showing a relatively homogeneous geneticstructure in geographically close populations.Assignation tests correctly assigned high percentages of individuals to their originalpopulations and groups,and also confirmed the existence of genetic differentiation among C.plicatula populations.The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservationof biodiversity for the species.     

中国天竺鲷属鱼类的矢耳石形态特征

利用采自我国近海的105尾标本,分析了细条天竺鲷、宽条天竺鲷、黑鳃天竺鲷、黑边天竺鲷、垂带天竺鲷、四线天竺鲷、半线天竺鲷、斑鳍天竺鲷等8种天竺鲷属鱼类的矢耳石形态特征。8种鱼类矢耳石前部均较尖,后部略圆钝,背部呈折线状,腹部呈圆弧形,背部中央有缺刻,听沟宽阔;8种鱼耳石长宽比的比值为1.35~1.63,矩形趋近率为0.67~0.77,充实度为14.77~24.99。以上述三种形态学参数为基础进行聚类分析,可将8种鱼类分为3组:细条天竺鲷、宽条天竺鲷、黑边天竺鲷、黑鳃天竺鲷为一组;垂带天竺鲷、四线天竺鲷、半线天竺鲷为一组;斑鳍天竺鲷为一组。耳石听沟边缘走向、宽度以及耳石外部边缘光滑度等轮廓特征在8种天竺鲷属鱼类间呈显著的种间差异。以这些形态特征为基础,编制了8种天竺鲷属鱼类的检索表,检索表表现的种间分类关系特征与聚类分析结果基本一致。     

基于线粒体16S rDNA和COΙ基因探讨中国近海黄姑鱼类的分子系统进化关系

为探讨中国近海黄姑鱼类的系统进化关系,通过PCR扩增了黄姑鱼(Nibea albifora)、浅色黄姑鱼(N.coibor)、日本黄姑鱼(N.japonica)和状黄姑鱼(N.miichthioides)等4种黄姑鱼类的16S rDNA和COΙ基因片段并进行了序列测定,计算其种间及种内遗传距离并结合来自GenBank的13种石首鱼科鱼类的相应基因片段序列构建分子系统树。结果表明:(1)利用16S rDNA片段可以对4种黄姑鱼类进行分子鉴定;但是基于COΙ基因片段计算的日本黄姑鱼和状黄姑鱼的种间遗传距离为0.002—0.005,尚未达到种间分化水平,应用该基因片段进行这两种鱼的分子鉴定值得商榷;(2)分子系统树显示日本黄姑鱼和状黄姑鱼与黄姑鱼和浅色黄姑鱼处于不同的系统发育阶元,日本黄姑鱼和状黄姑鱼与白姑鱼属鱼类聚为同一类群,支持形态学上将日本黄姑鱼和状黄姑鱼划归为白姑鱼属的分类学观点。     

东海蛾螺属二新记录种(腹足纲,蛾螺科)

在整理近年来东海采集的蛾螺科Buccinidae标本时,鉴定出属于蛾螺属Buccinum Linnaeus,1758的两个中国新记录种:(1)白肋蛾螺Buccinum leucostoma Lischke,1872,采自于东海300—400m深的泥沙质海底;(2)古式蛾螺Buccinum koshikinum Okutani,1988采自于东海400m深的泥沙质海底。文中分别对这两个新记录种的形态特征、生活习性等进行了描述,并与相似种进行了分类学讨论。此外,文中还列出了中国海已报道的其他蛾螺属种类。     

鞘藻属和毛鞘藻属营养细胞分裂方式的比较研究

于 1 999年 7月在湖北省仙桃市湿润土壤中采得一种鞘藻 ,经培养和电镜观察 ,发现该株鞘藻营养生长主要依赖位于丝状体最上面的细胞分裂 ,分生出的子细胞在该细胞亚顶端的胞壁破裂点掀开一个帽子 ,此帽子便附着于细胞侧壁上 ,这在鞘藻属中为首次报道 ,与 2 0 0 0年 1 2月在武汉纸坊青龙山水库采得的一种毛鞘藻的基细胞也附着相同的帽子结构。观察结果表明 ,该属基细胞亦具有明显的居间分裂能力。在观察这两属营养细胞居间分裂的同时 ,详细报道了这一种特殊的细胞分裂———顶端分裂 ,详细比较了这两属的营养细胞分裂方式 ,并对其分裂方式的机制进行探讨。结果表明 ,这两属细胞在细胞分裂方式上有极为亲密的亲缘关系 ,但毛鞘藻属更复杂、独特的营养细胞分裂显示它的进化地位比鞘藻属高。     

南海晚第四纪的硅藻遗体及其地质意义 Ⅴ.沟纹藻属

硅藻门（Bacillariophyta）沟纹藻属（LiriogrammaKolbe）在我国是1个新记录的属。该属最先由Kolbe（1954）在西太平洋赤道区（2°52＇S，89°50＇W，水深3225m）的柱状样沉积物中找到1种，订名L．petterssonii。之后，又在1955年从大西洋（0°7＇N，18°12＇E；0°8＇N，16°19＇E）和太平洋（6°44＇N，129°28＇W；2°52＇S，89°5＇W）的柱状样沉积物中找到另1种，订名L．hustedtii。笔者在南海第四纪的一个柱状样，距今约120ka（晚更新世）至11ka（全新世）的沉积物里找到了迄今该属已记录的2个种。此外，还记录了新组合的种：Liriogrammasarcophagu（Wallich）nov．comb．