Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

山东南部沿海冬季小型底栖生物的初步研究

利用统计法、经验模型和多元聚类分析法和数学分析软件对2006年12月908项目冬季航次山东南部海域(36°15′—36°45′N,120°45′—122°00′E)小型底栖生物调查所获得的数据进行分析,获得该调查海域冬季小型底栖生物的平均丰度为(657.43±214.60)ind/10cm2,平均生物量为(847.15±427.63)μgdwt/10cm2,平均生产量为(7624.32±3848.64)μgdwt/(10cm2·a-1);还对N/C、Simpson优势度指数、Margalef多样性指数、Pielou均匀度指数和香农-威纳指数进行了计算。与2006年8月908项目夏季航次同一海域调查数据进行了比较,得到小型底栖生物的平均丰度及其优势类群线虫丰度冬季明显低于夏季,而生物量和类群多样性与夏季相当。同时,依据丰度指标对山东南部研究海域进行了初步的生态学划分,呈现出较明显的地域性。     

曲线拟合法在山东流动重力资料处理中的应用

利用曲线拟合法对山东流动重力部分具有趋势性变化的异常测点进行了拟合改正,获取更可靠的重力变化信息,并对趋势性变化显著的测点进行了分析和讨论。     

A landscape pattern analysis method based on boundaries and nodes: A case study in upper Minjiang River, China

Traditionally,patch-based analysis at the landscape scale fragmentation has been used in the study of landscape ecology while the study of boundary and node has not been considered as much detail until recently.This study investigated the possibility of applying boundary-and node-based methods in landscape pattern analysis to the upper reaches of the Minjiang River in the southwestern China.Boundary-based and node-based landscape indices were selected to be used in analyzing changes in landscape patterns,and the results were compared with analysis using traditional pattern indices.We compared the responses of patch-area-based,boundary-length-based and node-number-based indices,and concluded that boundary-based and node-based indices are more sensitive to disturbance than patch-based indices with various patterns,and node-based indices are even more sensitive than boundary-based ones.Thus,the results suggest that boundary-based and node-based pattern analysis methods provide helpful supplementary information to traditional patch-based pattern analysis methods.The results about pattern dynamics of landscapes in the upper reaches of the Minjiang River based on boundaries and nodes showed that with human disturbance,the dominance of forest landscape was weakened by other landscape types; thus the landscape pattern of the study area became more homogeneous and the boundary network became more complex.These changes further augmented disturbance interfaces in the landscape and increased the possibilities of further landscape fragmentation.     

未知DEM下的机载SAR图像几何校正方法研究

针对机载环境复杂,不确定因素众多,与图像相对应的DEM不能精确获得等影响校正的不利因素,研究基于先验条件而不依赖DEM的机载SAR图像几何校正方法.从产生畸变的原理和校正过程的本质出发,将不同的几何校正理论在成像原理层面进行统一;在此基础上,分析精确校正所需要的条件,并提出当条件不能满足时的基于统计模型的几何校正方法;继而提出基于不同先验条件的校正方案决策模型.仿真实验及基于配准区域的保真度评价结果验证了所提方法的有效性.     

局部最优估计的参数求解方法

结合了飞行器轨道拟合的实际问题,介绍了在约束条件下基于局部最优估计的参数求解,阐述了其经验和方法。     

半参数估计与LS估计的有效性比较

建立半参数回归模型,将系统误差加入,推导出了模型正则化矩阵时参数平差的计算方法,并用直接法得到了参数、非参数的估计量,在MSE准则下与最小二乘估值(简称LS估值)进行比较,证明了半参数估值的有效性。     

公用机房的硬盘保护措施

在深入分析硬盘结构及硬盘工作原理的基础上,详细阐述了用软件实现对公用机房硬盘的保护原理和方法,并给出了编程思路。     

台阶式沉井等效墩宽计算方法探究

桥梁工程中存在形状各异的桥墩,在研究不同截面桥墩的局部冲刷特性时,桥墩尺寸的定量描述非常重要。以台阶式沉井结构为研究对象,梳理并分析了国内外相关等效桥墩宽度计算方法,发现各类公式本质上为计算不同条件下的桥墩平均阻水宽度。开展了台阶式沉井局部冲刷试验研究,基于试验数据对比分析了各类等效墩宽计算方法的计算结果,发现Melville和Raudkivi的等效墩宽计算公式能够较好地估算考虑动床条件下台阶式沉井的平均阻水宽度;进一步收集了国内外193组台阶式沉井类结构的局部冲刷试验数据,数据集统计分析结果显示Melville和Randkivi的等效墩宽计算公式能够较为准确且保守地量化大多数台阶式沉井类结构的截面非均匀性;最后基于数据集冲深墩宽比统计结果对该公式近似方法的合理性进行了讨论。