Application of rotifer Brachionus plicatilis in detecting the toxicity of harmful algae

The toxicity of seven major HAB (harmful algal bloom) species/strains, Prorocentrum donghaiense, Phaeocystis globosa, Prorocentrum micans, Alexandrium tamarense (AT-6, non-PSP producer), Alexandrium lusitanicum, Alexandrum tamarense (ATHK) and Heterosigma akashiwo were studied against rotifer Brachionus plicatilis under laboratory conditions. The results show that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6), or A. lusitanicum could maintain the individual survival and reproduction, as well as the population increase of the rotifer, but the individual reproduction would decrease when exposed to these five algae at higher densities for nine days; H. akashiwo could decrease the individual survival and reproduction, as well as population increase of the rotifer, which is similar to that of the starvation group, indicating that starvation might be its one lethal factor except for the algal toxins; A. tamarense (ATHK) has strong lethal effect on the rotifer with 48h LC₅₀ at 800 cells/mL. The experiment on ingestion ability indicated by gut pigment change shows that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6) and A. lusitanicum can be taken by the rotifers as food, but A. tamarense (ATHK) or H. akashiwo can be ingested by the rotifers. The results indicate that all the indexes of individual survival and reproduction, population increase, gut pigment change of the rotifers are good and convenient to be used to reflect the toxicities of HAB species. Therefore, rotifer is suggested as one of the toxicity testing organisms in detecting the toxicity of harmful algae. Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-208), National Natural Science Foundation of China (No. 40821004, U0733006) and National Basic Research Program of China (973 Program, No. 2001CB409700)     

Experimental Study on the Interspecific Interactions Between the Two Bloom-Forming Algal Species and the Rotifer Brachionusplicatilis

The interspecific interactions between the rotifer Brachionus plicatilis and two harmful algal blooms （HAB） species were investigated experimentally by single culture method. B. plicatilis population and the growth of the two algae were compared at different algal cell densities. The results demonstrated that the B. plicatilis obtained sufficient nutrition from Prorocentrum donghaiense to support net population increase. With exposure to 2.5×10^4 cells mL^-1 of P. donghaiense, the number of B. plicatilis increased faster than it did when exposed to other four algal densities （5, 10, 15 and 20 × 10^4 cells mL^-1）, and the increase rate of B. plicatilis population （r） at this algal density was 0.104 ± 0.015 rd^-1. Cell densities of P. donghaiense decreased due to the grazing of B plicatilis. In contrast, Heterosigma akashiwo had an adverse effect on B. plicatilis population and its growth was largely unaffected by rotifer grazing. In this case, B. plicatilis population decreased and H. akashiwo grew at a rate similar to that of the control.     

Experimental Study on the Interspecific Interactions Between the Two Bloom-Forming Algal Species and the Rotifer Brachionus plicatilis

The interspecific interactions between the rotifer Brachionus plicatilis and two harmful algal blooms(HAB) species were investigated experimentally by single culture method. B. plicatilis population and the growth of the two algae were compared at different algal cell densities. The results demonstrated that the B. plicatilis obtained sufficient nutrition from Prorocentrum donghaiense to support net population increase. With exposure to 2.5×104 cells mL-1 of P. donghaiense,the number of B. plicatilis increa...     

基于虚拟连接点模型的机载LiDAR系统安置误差自检校

利用激光扫描直接定位的严格模型,定量分析LiDAR系统安置误差对激光脚点定位精度的影响,设计了一种安置参数自检校方法提高LiDAR数据条带间相对精度,由于离散采样的激光脚点之间不存在真实的同名连接点,提出了虚拟点模型,将虚拟连接点与真实激光脚点联系起来,并定义了两组规则从激光脚点坐标计算出连接点坐标。采用安阳市内真实LiDAR数据进行试验,证明了本文介绍的自检校方法的可行性和有效性,获得的检校参数稳定有效,可直接对原始LiDAR条带进行纠正,补偿系统安置误差。     

八臂仙母虫(Eoandromeda octobrachiata)新材料及其意义

在中国贵州江口地区陡山沱组新发现的化石材料表明,伊迪卡拉/震旦纪(Ediacaran/Sinian)的宏体化石——八臂仙母虫Eoandromeda octobrachiata的原始生活状态可能为一个较高的圆锥形生命体,具有沿着侧面锥状螺旋排列的八条旋臂.旋臂表面保存有与栉水母动物的栉板可能同源的横向有机质条带,规则排列...     

点燃希望火炬的人生——记东北煤田地质局副总工程师、一○一勘探队队长黄贵

2011年7月8日,位于辽北付家镇境内的CQ2#煤层气参数兼生产试验井井场内外彩旗飘舞,崭新的多功能履带式煤层气钻机傲然挺立。由东北煤田地质局副总工程师、一○一队队长黄贵主持的＂辽宁省昌图县古榆树煤层气整装勘查开工仪式＂在这里隆重举行。     

三桩筒型基础平台的结构和拖航稳性分析与应用

以渤海湾应用的典型三桩筒型基础为例,通过结构静力分析和拖航稳性分析,推导出结构浮运重量与吃水对应关系,为结构设计和浮运施工方案提供理论依据。现场施工证明了计算分析的正确性,并对渤海近年该典型结构海上成功应用实例进行介绍,以期开阔工程技术人员海上简易平台设计思路,为极浅海海洋工程技术提供有益参考。     

基于模型试验的海洋核动力平台定位系统解脱作业关键指标分析

海洋核动力平台定位系统采用软刚臂单点系泊方式,属于多铰连接的单点系泊装置。定位系统在长期海上运营过程中,会面临结构维护、改造、更换等作业,解脱作业的安全性是定位系统乃至核动力平台安全的重要组成。借鉴国内外海洋石油平台解脱作业的成功案例,首先给出了核动力平台定位系统解脱作业的流程,并结合解脱作业故障树的风险传递路径给出关键风险指标;然后基于相似理论搭建了定位系统1∶9缩尺比模型试验平台,真实模拟了定位系统全流程解脱作业,验证了定位系统解脱作业的可行性与有效性;基于实时测量数据分析提升缆绳在限位状态与解脱作业时的张力响应、法兰解脱时系泊腿下部万向节振动响应、系泊刚臂下放姿态等关键指标。研究结果表明物理试验模拟方法可以准确表征解脱作业过程的关键节点,并为量化作业指标、优化作业流程提供数据支持,同时相关试验分析方法可以为其他海洋装备的解脱、安装等提供参考。     

区域协同发展背景下边缘城市的空间治理与融入策略——以广东省中山市为例

中国推进区域协同发展战略的核心要点在于统筹各区域当中边缘城市的发展,以实现全区域共同富裕。与此同时,在知识经济的背景下,高新技术产业开发区已成为各城市参与区域分工的重要场域空间。因此,随着区域协同发展战略的不断深化,边缘城市如何依托高新区带动全市融入区域一体化进程,已成为一个值得关注的话题。以广东省中山市为例,使用定性研究方法探究边缘城市通过一定的空间治理和融入策略来服务构建区域新发展格局的具体过程。研究发现：① 中山市积极构建高新区的“超地方性”,强调空间治理的目标是服务区域协同发展;② 推动多个城市职能尺度上移是边缘城市整体融入区域的重要策略,是中山市避免被继续边缘化的有效途径。