Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

基于EMD与神经网络的机械故障诊断技术

经验模式分解 (EMD)是分析非线性、非平稳信号的有力工具 ,它将信号分解为突出了原信号的不同时间尺度的局部特征信息的内在模函数 (IMF)分量。本文通过将各 IMF分量输入到 BP网络中进行训练学习和故障诊断 ,比直接输入原信号可以提高 BP网络对故障诊断的准确率 ,而且减少了训练时间。     

ECOM模式在丁字湾的应用

应用河口、陆架和海洋模式 (ECOM模型 ) ,引入干湿网格法模拟潮滩涨落的改进 ,并建立丁字湾及近岸海域的三维变动边界潮流模型。该模型考虑了湾口拦门沙、湾内水道和人工围海等地形特点。计算结果与实测值比较符合良好 ,较好地刻画出丁字湾 M2 分潮潮流场的时空分布特点。     

长期非平稳荷载调谐质量减振阻尼器优化设计

研究外荷载为长期非平稳随机过程。考虑长期荷载的特性 ,采用 1个概率谱密度函数来反映长期非平稳随机荷载及其特征 ;概率谱密度函数是基于大量的一般谱密度函数的统计特性获得。以延长结构的抗疲劳使用寿命为目标函数 ,提出了调谐质量减振阻尼器的优化设计方法 ,这在实际工程中有着极为广阔的应用前景。本文旨在从理论上发展长期非平稳随机荷载作用下调谐质量减振阻尼器的优化设计方法 ;文中采用长期波浪实测数据 ,给出了 1个数值算例说明整个设计过程。     

一种腐屑食性鱼类对生态系统氮循环的影响

研究表明 :腐屑食性鱼类——梭鱼对环境中有机氮的摄入能力随体重的增加而增加 ,随温度的升高 ,体重系数显著减少。摄入氮 (CN,mg/ d)与体重 (W,g)和温度 (T,℃ )的数值关系可用如下模型表示 :CN=0 .6 370 W1 .0 642 ln(3.70 0 8T)。体重为 1 0 0 g的梭鱼每日可消耗环境中 332 mg氮有机质。梭鱼氮的吸收效率主要受温度的影响 ,而氮的转化效率与体重呈显著的正相关关系。随体重的增加 ,排泄氮所占比例降低     

Responses of CO2 in surface water in subtropical West Pacific to El Niño event

In order to investigate the relationships between the change of TCO2, △PCO2 and SST, current, upwelling and biological activities during El Nin(～)o event in the subtropical Pacific, the responses of TCO2 and △PCO2 in surface water in the subtropical Pacific during El Nin(～)o and La Nina have been simulated using a three-dimension carbon cycle model with biota pump. The results of numerical simulations show that TCO2 in sea water increases with reducing of SST during mature phase of El Nin(～)o in the subtropical West Pacific . At the same period , the Kuroshio in this region was weakened , the zonal currents were divergence , the upwelling carried the water with high concentrations of CO2 to the sea surface , so both of TCO2 and △PCO2 in surface water were increased . But TCO2 and △pCO2 were decreased during La Nina period. These simulated results confirmed the observations in 1982/1983 , 1986/1987 ,1991/1995 and 1997/1998 El Nin(～)o events .     

罗源湾水母类的生态研究

样品于1986年11月至1987年10月在福建省罗源湾10个站位采集。经鉴定有52种水母。它们可分为沿岸暖水、沿岸暖温和大洋广布三种生态类群。水母类总数量和种类数的季节分布呈单周期,两者高峰均出现在7月。低谷分别出现于11月和1月。在平面分布上,以拟细浅室水母Lensia subtiloides占优势的月份,数量从湾口向湾顶递减,而以球型侧腕水母Pleurobrachia globosa占优势的月份,数量却递增。文中还讨论了水母类的分布和环境因素的关系。     

基于MAPGIS的市政设施数据模型研究

本文主要介绍了市政基本设施的组成,提出了基于MAPGIS基本数据组织的市政设施数据模型,并讨论了市政设施数据模型的实现。从而,利用该数据组织模型可实现城市级的市政设施管理的综合集成与共享,提高市政设施管理和养护水平。     

基于DEM的SAR图像模拟

首先阐述了研究基于数字高程模型(DEM)的SAR图像模拟的重要性。然后针对SAR图像的几何特点,并顾及地面坡度、成像距离等因素对雷达波后向散射强度的影响,设计了基于强度积分的SAR图像模拟方案。根据Leberl构像模型计算SAR图像的像点坐标,采用强度积分策略对各点处的强度进行模拟,并进行归一化处理。通过仿真实验得到了满意的SAR模拟图像,验证了该模拟方案的正确性和有效性。     

GIS支持下的煤层气目标区模糊综合评价模型

地理信息系统(GIS)以空间数据库技术为核心,将空间要素与统计数据有机地结合在一起,具有直观灵活、分析功能强大、制表制图方便等优点。根据中国煤层气(CBM)目标区的特点,建立了4种原则下用于不同情形的煤层气目标区评价模型:第1种模型为全面考虑各个因素原则,第2种模型为只考虑重点因素原则,第3种模型为着重考虑重点因素原则,第4种模型为既全面考虑又兼顾重点因素原则。根据这4种模型,在常用GIS软件MapGIS基础上进行了二次开发并实现模型,以华北聚气区煤层气为例进行综合评价,目标区评价结果与实际情况基本相符。