2004年夏季台湾浅滩及其邻近海域悬浮体成分与分布特征

本文根据2004年7月25日至8月10日在台湾浅滩及其邻近海域获取的悬浮体样品,采用了"微孔滤膜法"取得数据,利用偏光显微镜、体视显微镜和扫描电镜及能谱分析,结果表明:悬浮体含量在水平分布上为由近岸向外逐渐降低,总体上表层悬浮体含量高于底层;悬浮体的成分包括粗颗粒组份(它生矿物碎屑、自生矿物和生源碎屑)和细颗粒组份(无机矿物颗粒和超微生源颗粒),其中表层矿物碎屑分布特征为由岸向外递减,底层矿物碎屑则为近岸低、浅滩区高和浅滩外侧低;自生矿物和表层生源碎屑都具有自岸向外增加的特点;细颗粒组份中无机矿物颗粒为主,超微生源颗粒含量较少.研究区悬浮体分布受水文因素、物质来源及海底地形的影响,中国大陆陆源输入影响研究区悬浮体成分及其分布特征.     

水产饲料中镉的存在形态及其在养殖动物体内累积状况的研究

镉是对动物和人类健康有害的重金属元素,其毒性与存在形态密切相关.本文对水产饲料和原料中的含镉量、及其在不同提取剂的提取量进行测定,探讨其主要存在形态;测定结果表明水产配合饲料及其原料中的镉主要以蛋白结合态存在.以乌贼内脏粉为镉源调制的不同含镉量饲料对鱼、虾、蟹的饲喂对比试验,结果进一步证实,以Cd-MT 为主体的有机结合态镉对水产动物的毒理效应和代谢作用与离子态镉(Cd2 )存在着明显的差异,其在水产动物肌肉的再累积不明显,未发现对养殖动物的毒害作用和Cd的累积放大现象.     

微藻育种的研究现状及前景

概述了微藻育种的研究现状及前景,并比较了几种主要育种方法(选择育种、诱变育种、细胞融合、基因工程)的优缺点:1)选择育种是一切育种实践的基础,虽然简单易行、安全性好,但藻株发生变异的机率较小,育种周期长,选育的微藻普遍存在遗传性状不稳定的缺点;2)诱变育种使用最广泛,此法具有所需仪器设备简单、操作方便、成本低、效率高等优点,但该技术对遗传物质DNA的操作不具有基因工程那么强的针对性,而且在操作时对人体及环境有一定的危害性;3)细胞融合既可以提高物种变异频率,也可以缩短育种周期,拓宽育种领域,从而提高育种水平,应用前景广阔.但是该技术在微藻育种中的应用还较少;4)基因工程进行新藻种的选育具有目标明确、针对性强等特点,但其安全性还有待进一步验证.另外,本文还对叶绿素荧光技术在微藻育种中的应用进行了初步探讨.     

夏季台湾浅滩周边海域叶绿素a荧光垂直分布对温盐的响应分析

根据2004年7-8月台湾海峡南部台湾浅滩周边海域的温度、盐度和叶绿素a荧光的观测资料,分析了调查期间温度、盐度、叶绿素a荧光垂直分布特征及其相互关系.结果表明,温盐环境因子的变化对叶绿素a荧光垂直分布有重要影响.夏季浅滩西侧近岸区出现的温盐跃层控制着叶绿素a荧光的垂直分层特征;浅滩南部陆架斜坡区中下层涌升水较强,于温盐跃层之中出现叶绿素a荧光的单峰;浅滩区水浅,基本上无(或弱)温盐跃层,叶绿素a荧光的垂向分布也较均匀;在浅滩区边缘,受上升流与浅滩强潮混合作用影响,叶绿素a对温盐的响应呈线性关系.     

Denitrification in Qi'ao Island coastal zone, the Zhujiang Estuary in China

Samples of sediments and the overlying water were collected in the Qi'ao Island coastal zone, the Zhujiang (Pearl River) Estuary (ZE). Denitrification rates, sediment oxygen demand (SOD) , and fluxes of inorganic nitrogen compounds were investigated with N2 flux method, using a self-designed continuous flow through and auto-sampling system. The results indicate that the denitrification rates varied between 222 and 908 μmol/(m2·h) with an average of 499 μmol/(m2·h). During incubation, the sediments absorbed dissolved oxygen in the overlying water with SOD ranging from 300 to 2 363 μmol/(m2·h). The denitrification rates were highly correlated with the SOD (r2 =0.77) regardless of the NO3- + NO2- concentrations in the overlying water, organ- ic carbon contents in sediments and water temperature, suggesting that the SOD was probably the main environ-mental factor controlling the denitrification in the Qi'ao Island coastal zone. There was a net flux of NO3- + NO2-into the sediments from the overlying water. The NH4+ flux from sediments into water as the result of mineraliza-tion was between 12. 3 and 210. 3 μmol/(m2·h) ,which seems limited by both organic carbon content in sedi-ment and dissolved oxygen concentration in the overlying water.     

东北季风期海南岛三亚湾初级生产力分布特征及其影响因素

根据2006年10月和2007年1月的监测资料,对三亚湾在东北季风初始期和盛行期初级生产力的分布特征及其受水体环境的影响因素进行了分析。三亚湾秋、冬季表层潜在初级生产力平均值分别为6.81mgC·m-3·h-1和7.47mgC·m-3·h-1;水柱初级生产力平均值分别为504.96mgC·m-2·d-1和495.33mgC·m-2·d-1。东北季风期间,三亚湾水体垂直混合作用加强,层化现象减弱,水动力过程和营养盐等环境因子是该海湾初级生产力分布特征和季节变化的重要调控因素;其中冬季较高的DIN分布对表层初级生产力有潜在促进作用,而水体扰动增强、真光层深度下降则导致水柱初级生产力低于秋季。三亚河口近岸水域秋、冬季均为营养盐、叶绿素a及初级生产力的高值区,其中表层潜在初级生产力在秋季和冬季分别达到了45.80mgC·m-3·h-1和39.27mgC·m-3·h-1,表明调查期间三亚河营养盐输入对近岸水体浮游植物初级生产过程的显著影响。     

谷氨酰胺二肽对日本对虾血清生化指标、肝胰腺细胞凋亡及肠粘膜形态的影响

采用饲养对比试验方法,研究了谷氨酰胺二肽（Gin二肽）对日本对虾血清生化指标、肝胰腺细胞凋亡和肠绒毛的影响及对虾体健康的作用。结果表明,饲喂Gin二肽各组的PO、LSZ、ACP含量均显著（P〈0．05）高于对照组,添加0.5％和1．0％Gin二肽组的SOD和ALP含量也显著（P〈0．05）高于对照组：添加1．0％Gin二肽的TP含量显著（P〈0．05）高于其它各组。血清TP、PA、UN、CHO、PO、LSZ、SOD、ALP、ACP和LDH等各项指标均随Gin二肽添加量的提高而呈现增加趋势。试验建立了流式细胞术检测对虾肝胰腺细胞凋亡率的方法;肝胰腺细胞凋亡率随着日粮Gin二肽添加量的增加而显著降低（P〈0．05）。肠道切片显微观测结果表明,添加1．0％的Gin二肽能显著增加日本对虾肠绒毛高度（P〈0．05）。饲料中添加Gin二肽可显著提高日本对虾血清中的溶菌酶、抗氧化酶及磷酸酶活性,降低肝胰腺细胞凋亡率,增加肠绒毛高度,改善虾体健康状况。     

台风对海洋叶绿素a浓度影响的定量遥感初探

研究台风对海洋水色环境的影响,是目前海洋遥感技术又一应用领域。由于台风期间天气恶劣,遥感资料较少,国际上主要针对个别典型台风,研究其对海表温度、海洋叶绿素a浓度及初级生产力的影响,很少通过遥感资料系统地对这一影响进行定量分析和建模。自2000到2007年,过境中国近海以及西北太平洋海域台风近百次,作者通过系统地分析这期间MODIS,SeaWiFS的3A级叶绿素a浓度数据,结果发现:(1)台风促进了相应海域叶绿素a浓度的大幅增长,总体上平均增长约1.426倍,个别区域在5倍以上,同时,该增长一般延后3~6d,在7~10d后恢复到原来的水平;(2)进一步对这些数据进行一元统计线性回归,发现叶绿素a浓度增长比(R_chl-a)与台风影响因子(Tsub>w)满足如下关系:R_chl-a=0.0012Tsub>w+1.017,其相关系数达0.8;(3)台风期间叶绿素a浓度与无台风时叶绿素a浓度之间有很强的线性关系,其关系满足:C_chl-a=1.2367C_0chl-a+0.0636,且相关系数高达0.98。这一初步研究结果对进一步通过遥感手段深入研究台风对海洋水色环境的影响有借鉴意义。     

Distribution patterns of chlorophyll <Emphasis Type="Italic">a</Emphasis> in spring and autumn in association with hydrological features in the southern Yellow Sea and northern East China Sea

Two field studies were conducted to measure pigments in the Southern Yellow Sea (SYS) and the northern East China Sea (NECS) in April (spring) and September (autumn) to evaluate the distribution pattern of phytoplankton stock (Chl a concentration) and the impact of hydrological features such as water mass, mixing and tidal front on these patterns. The results indicated that the Chl a concentration was 2.43±2.64 (Mean ± SD) mg m^?3 in April (range, 0.35 to 17.02 mg m^?3) and 1.75±3.10 mg m^?3 in September (from 0.07 to 36.54 mg m^?3) in 2003. Additionally, four areas with higher Chl a concentrations were observed in the surface water in April, while two were observed in September, and these areas were located within or near the point at which different water masses converged (temperature front area). The distribution pattern of Chl a was generally consistent between onshore and offshore stations at different depths in April and September. Specifically, higher Chl a concentrations were observed along the coastal line in September, which consisted of a mixing area and a tidal front area, although the distributional pattern of Chl a concentrations varied along transects in April. The maximum Chl a concentration at each station was observed in the surface and subsurface layer (0–10 m) for onshore stations and the thermocline layer (10–30 m) for offshore stations in September, while the greatest concentrations were generally observed in surface and subsurface water (0–10 m) in April. The formation of the Chl a distributional pattern in the SYS and NECS and its relationship with possible influencing factors is also discussed. Although physical forces had a close relationship with Chl a distribution, more data are required to clearly and comprehensively elucidate the spatial pattern dynamics of Chl a in the SYS and NECS.     

小球藻和铜绿微囊藻的高浓度Chl-a高光谱定量模型

本研究将地物高光谱遥感技术应用于室内实验,从而得到小球藻和铜绿微囊藻的高光谱特征.通过多种半经验方法,如单波段、波段比值和微分法,建立了两藻种最优的Chl-a高光谱定量模型,并与室外情况进行了对比.结果表明:小球藻的最优定量模型为Chl-a=174.6 1138292(R703) 2.3(109[(R703)]2(p＜0.01),相应的方法适宜性为:一阶微分法＞单波段法＞波段比值法;铜绿微囊藻的最优定量模型为Chl-a=5299164(R757)1.9773(p＜0.01),相应的方法适宜性为:单波段法＞波段比值法＞一阶微分法;从高光谱特征来看,小球藻在540 nm和700 nm附近存在明显的特征波峰,其位置随Chl-a浓度增大而向长波方向偏离,铜绿微囊藻在530 nm、660 nm和700 nm附近存在3个较强的特征波峰,在610 nm和680 nm附近存在明显的波谷;与以往室外研究不同的是铜绿微囊藻的反射率在400-500 nm之间的R值并不低,是因为没有非藻类颗粒物的影响,总吸收明显降低.