负载姜黄素β-环糊精功能化纳米银诱导HepG2细胞凋亡机制

【目的】考察负载姜黄素的环糊精功能化纳米银(Ag@β-CD@Cur)诱导HepG2细胞凋亡的分子机制。【方法】采用荧光法测定Caspase 3酶活力,利用Western blotting实验测定Akt-p53-caspase3及其信号通路上的关键性的上游蛋白蛋白激酶B(Akt)蛋白、中游蛋白p53。【结果】Ag@β-CD@Cur能够诱导caspase3活性显著性的升高。Western blotting实验发现,Ag@β-CD@Cur能够通过下调Akt蛋白表达量和上调p53蛋白表达量来降低caspase 3的表达从而诱导细胞发生凋亡。【结论】Ag@β-CD@Cur通过Akt-p53-caspase3信号通路诱导Hep G2细胞凋亡。     

化痰通络汤加减治疗急性脑梗死临床疗效的Meta分析

目的：系统评价化痰通络汤加减治疗急性脑梗死（ACI）的临床疗效。方法：计算机检索The Cochrane Library、Embase、Pubmed、中国知网（CNKI）、维普中文科技期刊数据库（VIP）、万方数据和中国生物医学文献数据库（CBM）中所有化痰通络汤加减治疗急性脑梗死的随机对照试验（RCTs）,并辅以手工检索相关核心期刊,追踪纳入研究的参考文献。结果：最终纳入14项研究,共1210例患者。Meta分析结果显示：与对照组的西医常规治疗方案相比,治疗组联合化痰通络汤加减治疗能有效提高ACI患者的临床疗效,总有效率的合并效应量和95%的置信区间为［OR=3.92,95%CI（2.71,5.68）,P＜0.00001］,合并效应量检验：Z=7.23;并能显著降低神经功能缺损评分和提高中医证候疗效,合并效应量分别为［SMD=-0.67,95%CI（-0.89,-0.46）,P＜0.00001］和［OR=3.88,95%CI（2.08,7.24）,P＜0.00001］。结论：基于纳入14项研究的Meta分析结果,联合化痰通络汤加减治疗急性脑梗死临床疗效优于常规西医治疗,值得临床推广。     

强心安神方对阿霉素致大鼠心肌损伤模型心肌细胞能量代谢的影响

目的：通过研究强心安神方对阿霉素性心肌损伤大鼠模型心肌酶、心肌细胞线粒体呼吸功能和细胞色素C氧化酶活性（COX）的影响,探讨该药的作用机制。方法：将Wistar大鼠分为正常对照组、模型组、西药组及强心安神方低、高剂量组,对除正常对照组外的其余组大鼠采用腹腔注射阿霉素法制备心肌损伤模型,予药物干预4周,检测各组大鼠血清心肌酶[包括肌酸激酶同工酶（CK-MB）及乳酸脱氢酶（LDH）的表达,提取大鼠心肌细胞线粒体测定线粒体呼吸功能（呼吸控制比,RCR）及COX活性。结果：与正常对照组比较,模型组CK-MB、LDH表达均升高,COX活性增加,差异有统计学意义（P＜0.01或P<0.05）;强心安神方高、低剂量组大鼠各项指标与西药组比较,差异均有统计学意义（P＜0.05）,强心安神方高、低剂量组之间各项指标差异无统计学意义（P>0.05）。结论：强心安神方对损伤心肌细胞的保护作用可能与其降低CK-MB及LDH、改善心肌细胞线粒体呼吸功能及COX活性有关。     

不同激素配伍对雨生红球藻(Haematococcus pluvialis)细胞生长和虾青素累积的调节作用、藻株差异及应用

利用生长素(NAA、3-IBA)和细胞分裂素(6-BA、kt)两类植物激素,对比研究了其不同配伍对4株雨生红球藻H0、H2、H3和H4细胞生长以及虾青素累积的调节作用.结果表明,尽管在不同株系之间存在一定的差异,但激素明显促进该藻细胞生长和虾青素累积,而对细胞大小没有产生明显影响.激素对红球藻细胞数量的增加主要是靠加快游动细胞阶段无性繁殖过程来实现的:而激素对虾青素含量的提高却是通过增加细胞数量和细胞内虾青素积累的协同作用结果,其中以细胞数量的增加为主.在激素单因素实验中,3-IBA对H0细胞生长增加效果最好,对H2、H3细胞生长增加效果较好的是kt,对H4细胞生长增加效果最明显的是NAA.与空白对照组相比,上述激素处理后细胞生长速度分别提高了1.95、1.54、4.25和1.78倍.经过激素多因素实验,H0、H2、H3和H4藻株的细胞生长增加效果分别是空白组的1.44、2.62、2.52、1.07倍,而虾青素含量分别为空白组的1.09、1.61、1.37、2-35倍.根据4株藻株的实验结果以及综合考虑各激素的市场价格,建议在规模化生产中,采用1.75mg/NAA和4mg/L 3-IBA以提高红球藻的培养效果.     

文蛤(Meretrix meretrix)幼虫发育过程中细胞凋亡和Caspase 功能分析

以文蛤为研究对象,利用TUNEL技术(Terminal-deoxynucleotidyl transferase mediated dUTP Nick End Labeling)检测了文蛤各发育阶段的幼虫中细胞凋亡的部位,利用Caspase原位标记分析了有活性的Caspase在幼虫中的分布情况,用Caspase抑制剂Z-VAD-FMK处理受精后4天和5天的变态前幼虫,同时以抑制剂的有机溶剂DMSO(Dimethyl sulfoxide)作对照,根据其对幼虫变态的影响,分析Caspase在文蛤幼虫变态中的作用.细胞凋亡检测结果显示,幼虫发育过程中发生细胞凋亡的部位主要在壳顶幼虫的面盘,说明细胞凋亡可能是文蛤幼虫变态过程中面盘退化的主要机制;在担轮幼虫至壳顶幼虫中都检测到有活性的Caspase,说明整个幼虫形态变化过程中都有Caspase的参与;用Caspase特异性抑制剂处理变态前幼虫,发现处理组幼虫变态率均显著低于对照组(P<0.05),说明Caspase在变态早期和晚期都有重要作用,同时发现DMSO对幼虫变态有诱导作用,可以在生产中加以利用.     

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

Prorocentrum donghaiense is an important harmful algae bloom (HAB) causing creature in China’s seas, and the conventional visual detection can not cope with long-term monitoring and high-throughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay (NPA-SH) was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, ...     

Effects of tributyltin （TBT） on enzyme activity and oxidative stress in hepatopancreas and hemolymph of small abalone, Haliotis diversicolor supertexta

We investigated the effect of tributyltin (TBT) exposure on the concentration of malondialdehyde (MDA) and the activity levels of the superoxide dismutase (SOD), catalase (CAT), and acid and alkaline phosphatase (ACP and AKP) enzymes in the small abalone, Haliotis diversicolor supertexta. We collected samples of the hepatopancreas and hemolymph 2, 6, 24, 48, 96, and 192 h after exposure to 0.35 μg (Sn)/L TBT. In the hepatopancreas, ACP activity was significantly higher in animals exposed to TBT 2, 24, and 96 h post-exposure compared with the control animals. AKP activity was also higher after 2 h, but SOD and CAT activity was unchanged. The concentration of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure. In the hemolymph of animals exposed to TBT, ACP activity was significantly lower than in the control animals 192 h post-exposure, whereas AKP activity was significantly lower 2 and 192 h post-exposure. Hemolymph SOD activity and levels of MDA were significantly lower than in the control animals 24 h after exposure but significantly higher after 96 h. Our results demonstrate that exposure to TBT cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph. SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone.     

Effects of iron electrovalence and species on growth and astaxanthin production of Haematococcus pluvialis

To increase the cell concentration and the accumulation of astaxanthin in the cultivation of Haematococcus pluvialis, effects of different iron electrovalencies (Fe²⁺-EDTA and Fe³⁺-EDTA) and species (Fe-EDTA, Fe(OH)_x^32x and FeC₆H₅O₇) addition on cell growth and accumulation of astaxanthin were studied. Results show that different iron electrovalencies have various effects on cell growth and astaxanthin accumulation of H. pluvialis. Compared with Fe³⁺-EDTA, Fe²⁺-EDTA stimulate more effectively the formation of astaxanthin. The maximum astaxanthin content (30.70 mg/g biomass cell) was obtained under conditions of 18 μmol/L Fe²⁺-EDTA, despite the lower cell density (2.3×10⁵ cell/ml) in such condition. Fe³⁺-EDTA is more effective than Fe²⁺-EDTA in improving the cell growth. Especially, the maximal steady-state cell density, 2.9×10⁵ cell/ml was obtained at 18 μmol/L Fe³⁺-EDTA addition. On the other hand, all the various species of iron (EDTA-Fe, Fe(OH)_x^32x, FeC₆H₅O₇) are capable to improve the growth of the algae and astaxanthin production. Among the three iron species, FeC₆H₅O₇ performed the best. Under the condition of a higher concentration (36 μmol/L) of FeC₆H₅O₇, the cell density and astaxanthin production is 2 and 7 times higher than those of iron-limited group, respectively. The present study demonstrates that the effects of the stimulation with different iron species increased in the order of FeC₆H₅O₇, Fe(OH)_x^32x and EDTA-Fe. Supported by Xiamen Scientific and Technologic Projects (No. 3052Z20031086), Xiamen University Alumni Association Foundation in Singapore, and the First National College Students Innovative Experimental Project