单环刺螠纤溶酶的分离纯化及其性质的初步研究

从单环刺中制得单一组分的纤溶活性纤溶酶,并对其性质进行初步研究.单环刺体组织液经离心,超滤,离子交换层析,凝胶过滤等方法进行分离纯化,制得单一洗脱峰酶组分,经Native-PAGE电泳分析,SDS-PAGE电泳分析和飞行质谱分析,鉴定了酶制品纯度和相对分子量,进行了体外溶血栓试验.所得纯品UFEIa水解酪蛋白的比活力为442.0 U/mg,纯化倍数为12.1倍,回收率为27.0%.UFEIa为单一组分,相对分子量约23 800 Da.体外溶血栓实验表明此纤溶活性蛋白酶与蚓激酶相比具有很好的溶血栓活性.     

一种水不溶灰树花菌丝体多糖(GF4A)硫酸酯化研究

以灰树花发酵菌丝体水不溶性多糖GF4A为原料,以三氧化硫三甲胺盐(SO3·Me3N)为酯化试剂,探讨了GF4A的硫酸酯化工艺.实验结果表明,当三氧化硫与GF4A中单糖摩尔比为5.8∶1、反应温度为80 ℃、反应时间6 h时,所得酯化产物(SGF4A)水溶性好、产率高.该产物硫酸酯取代度为1.6, 相对分子质量为88 kD.通过红外光谱及核磁共振碳谱分析表明,糖残基中所有C-6羟基以及部分C-4和C-2位羟基被酯化.体外实验表明,SGF4A具有较好的抗凝活性.     

Preliminary comparison of trace metals in coastal aerosols between Qingdao and Liverpool

Aerosol samples were collected at two coastal suburban stations, Qingdao （China） in 1995-1996 and Liverpool （U.K.） in 1995, respectively. The samples were analyzed to determine the concentrations of trace metals （Cr, Zn, Cu, Co, Ni, Pb, V, and Cd） as well as A1, Fe and Mn. Data were examined to understand the difference of trace metals in aerosols between coastal zones downwind the developing area （near the Yellow Sea） and developed region （near the Irish Sea）. The results show that most elements at Qingdao have levels 4-5 times higher than those at Liverpool, particularly for the crust-dominated elements （e.g. Al, Fe and Mn）. Moreover, the aerosol composition at Qingdao is higher in spring than in summer, underlying the influence of westerlies and local emissions in combination, whereas seasonal change of aerosol composition is not significant at Liverpool. The enrichment factors for the crustal source elements （EFcrust） at Liverpool are much higher than those at Qingdao. The contributions from the pollutant source （Rp） for some trace metals like Cu, Pb, Zn and Cd are 〉90% at Qingdao and Liverpool, suggesting overwhelming anthropogenic contributions to these metals. The contributions from crustal source （Re） for trace metals tend to increase with higher aerosol levels and Al concentration at Qingdao, indicating a good correlation between the crust-dominated component and the air mass. At Liverpool, the Rc values for trace metals are positively correlated with Al concentrations instead of with aerosol mass, suggesting that Al in aerosols represents the crustal component even though the aerosols come from different sources.     

Isotopic tracing of ore-forming source materials for Dexing porphyry copper deposit of Jiangxi, China

Dexing copper deposit is the biggest porphyry copper deposit in China. By researching isotopes of C, Si and Cu from the samples of Tongchang and Fujiawu ore-field, the authors found that δ^13 CpDB values of siderite were close to the δ^13 CpDB value of original magma; δ^30Si values of the samples at the ore-forming stage were close to the δ^30 Si value range of magma, δ^30Si values of partial samples were far away from it; Cu isotopic compositions of massive chalcopyrite formed at the early ore-forming stage are higher than that of veinal chalcopyrite formed at the later ore-forming stage. The results show that ore-forming materials were mainly derived from the porphyry body, and part of them were from wall rock materials.     

小秦岭金矿田金矿成矿物质来源及聚集规律探讨

通过金矿成矿元素的克拉克浓度、同位素等特征,运用统计、数学地质差别分析等方法,探讨小秦岭金矿田金矿成矿物质来源及聚积规律.     

基于Gabor变换的嵌入式气表自动识别系统

介绍一种基于嵌入式操作系统WinCE开发的天然气表字符自动识别系统。系统运用区域增长法和最大类间方差法对图像进行字符区域定位及字符分割,提出了将Gabor特征用于气表字符识别并讨论了Gabor滤波器参数优化设置方法。实验表明系统具有良好的识别效果及实时性。     

沙虫干呈味及功能性成分研究

对沙虫干抽提物成分的组成进行的研究结果表明：游离氨基酸含量ｗ为１０．３％,占抽提物含氮物质的７７．９％。抽提物组成中呈甘味的Ｇｌｙ（３．２％）、Ａｌａ（２．５％）和呈鲜味的ＩＭＰ（０．２７％）、Ｇｌｕ（０．２５％）、琥珀酸（０．３５％）等成分同沙虫干独特的风味有关,Ｎａ＾＋、Ｃｌ＾＋等无机离子同其呈味相关,此外,牛磺酸含量ｗ高达３．２％,是富含牛磺酸的一种海洋生物;精氨酸的含量ｗ亦较高,为１．２％     

沙虫干呈味及功能性成分研究

对沙虫干抽提物成分的组成进行的研究结果表明 :游离氨基酸含量 w为 10 .3% ,占抽提物含氮物质的 77.9%。抽提物组成中 ,呈甘味的 Gly(3.2 % )、Ala(2 .5% )和呈鲜味的 IMP(0 .2 7% )、Glu(0 .2 5% )、琥珀酸 (0 .35% )等成分同沙虫干独特的风味有关 ,Na 、Cl-等无机离子也同其呈味相关。此外 ,牛磺酸含量 w高达 3.2 % ,是富含牛磺酸的一种海洋生物 ;精氨酸的含量 w亦较高 ,为 1.2 %。     

【“漫话土地”之三十七】 解纷止争息田讼

“人生不可无田,有则仕宦出处自如,可以行志;不仕则仰事俯育,不致丧失气节。”在中国人的心目中,土地早已超越生产生活的物质层面,而上升为立身处世的精神依托。正是缘于土地之重要,因占有土地所引发的纠纷、诉讼乃至争斗,从古至今,从未歇止。