蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

某滑坡稳定分析及处理方案探讨

滑坡属于不良地质现象，是斜坡上的部分岩体和土体在自然或人为因素的影响下沿某一明显的界面发生剪切破坏向坡下运动的现象。当高速公路不得不通过某一滑坡时，对其稳定性进行分析和整治设计就显得尤为重要。以祁临高速公路灵石至霍州段窑深沟大桥东南窑深沟滑坡为实例，根据勘察资料，利用岩土力学知识，在公路修好后并运行的情况下，计算出了其稳定系数和推滑力，又以材料力学、结构力学等力学理论为基础，设计出抗滑桩和锚索抗滑桩等两种整治方案，经验证，效果良好。     

CARBON DYNAMICS OF WETLAND IN THE SANJIANG PLAIN

1INTRODUCTIONWetlandsplayanimportant roleintheprocessofcar-bonstorage.Thetotalcarbonstoredindifferentkindsofwetlandsisabout15%-35%ofthetotalcarboninthegloballandsoils(POSTetal.,1982;GORHAM,1991).Inaddition,wetlandsaresignificantnaturalsources fortheatmospheric CH4 (MOORE,1994).It isestimatedthatabout110×1012gCH4 originates fromanaerobicdecompositioninthenaturalwetlands,CH4 emission fromthenaturalwetlandsis15%-30%oftheglobalCH4 emission andtheCH4 emission from thepeat land at hi…     

一定要把人类的牧地变成鲸类的墓地？

谁操纵了鲸类的命运 鲸（Whale），地球上现存最大型的动物，也是最大型的哺乳动物，曾经广泛分布在世界各大洋的海洋霸主，但它们在用现代科技武装起来的人类面前，再也强大不起来了。如今，徘徊在灭绝边缘的鲸类除了面临海洋污染、船舶撞击、噪声污染、渔业误捕和渔网缠绕等因素造成的危险外，更主要的威胁来自于捕鲸船的围猎。     

实行生态移民改善生态环境--关于整合扶贫与生态整治的战略思考

经过几十年，特别是20世纪90年代扶贫攻坚计划实施，我国扶贫工作取得了巨大成就，但目前我国还有约3000万贫困人口，这些人口主要聚集在生态环境恶劣地区。事实上，以往正是由于这些地区人口活动造成毁林开荒，过度垦牧，严重恶化了生态环境。目前，我国已成为世界上水土流失最严重的国家之一，水土流失面积已达367万平方公里，约占国土面积的38％，并以每年1万平方公里的速度在增加。西北黄土高原是我国水土流失最严重的地区，长江流域也面对巨大的生态隐患。我国还是世界上沙漠化危害严重的国家之一。     

SIMULATION AND PREDICTION OF DEBRIS FLOW USING ARTIFICIAL NEURAL NETWORK

Debris flow is one of the most destructive phenomena of natural hazards. Recently, major natural haz-ard, claiming human lives and assets, is due to debris flow in the world. Several practical methods for forecasting de-bris flow have been proposed, however, the accuracy of these methods is not high enough for practical use because of the stochastic and non-linear characteristics of debris flow. Artificial neural network has proven to be feasible and use-fill in developing models for nonlinear systems. On the other hand, predicting the future behavior based on a time se-ries of collected historical data is also an important tool in many scientific applications. In this study we present a three-layer feed-forward neural network model to forecast surge of debris flow according to the time series data collect-ed in the Jiangjia Ravine, situated in north part of Yunnan Province of China. The simulation and prediction of debris flow using the proposed approach shows this model is feasible, however, further studies are needed.     

小波分析理论在GPS技术中的应用

在GPS数据处理中，存在着误差影响、影响波的干扰、周跳和数据量大等问题。误差影响和影响波的干扰实质是在接收卫星信号时受到其它因素的影响；周跳是由于卫星信号的失锁而造成信号的不连续；数据量大是因为GPS观测需要采样间隔小又连续观测所致。由于小波理论具有时频分析、波形分解、特征提取和快速小波变换等特性，应用小波变换和波形分解可以解决误差影响和影响波的干扰的问题；应用特征提取可以解决周跳检测问题；应用快速小波变换可进行数据压缩。     

马卡良335的微光变观测

介绍了马卡良 335分别发生于 1998年和 1999年的两次短时标光变。其中的一次是微光变 ,发生于 1999年 11月 2 0日 ,微光变区域的大小约 0 .80 7光时。还对微光变的可能机制进行了讨论     

浅论土地信息资源的合理利用

土地信息资源是与特定地块相关的各种信息，是土地管理工作处理的对象和结果。我国目前对土地信息资源的重要性认识还不够，缺乏技术人才，对土地信息资源的收集和利用存在缺乏市场机制和效率不高等问题。在土地管理过程中，可采取一定措施来合理利用土地信息资源。     

DISASTER ANALYSIS AND COUNTERMEASURES OF LAND SUBSIDENCE CAUSED BY COAL CUTTING IN CHINA

In recent years, many coal-producing countries have paid great attention to the land subsidence causedby coal cutting. In China, because of the dense population in coalfield areas, the land subsidence hazard is more seri-ous. After a brief analysis on the mechanism of land subsidence, this paper gives a comprehensive and systematical ac-count on all kinds of hazards caused by the land subsidence in China. The study shows that land subsidence has endan-gered land, buildings, traffic and communication lines, dykes and dams. It also causes damage to ecological and socialenvironment. In order to lessen the hazard of land subsidence, preventive measures should be taken to reduce the col-lapse amount, such as extraction with stowing, banded mining system, succession and coordination mining system, orhigh-pressure mudflow between rock strata. Measures of reinforcing or moving certain buildings should also be taken toreduce the destructive degree. In order to harness the subsidence land and bring them under control for fanning, mea-sures should be taken such as filling with spoil or fine breeze, excavating the deeper and covering the shallower land.