Effects of tributyltin at environmental levels on monooxygenase system of digestive gland in hard clam Meretrix meretrix

The effects on ethoxyresorufin O-deethylase （EROD）, NADPH-cytochrome c reductase and NADH cytochrorne b5 reductase activities of digestive gland in Meretrix meretrix exposed to tributyltin （TBT） at environmental levels （0.1,1.0,10.0 ng/dm as stannum concentration）,in experimental condition, were evaluated. The EROD, NADH cytochrorne b5 reductase activities were significantly inhibited after exposure to 10.0 ng/dm^3 TBT for 8 and 20 d, the NADPH cytochrorne c reductase activities were significantly inhibited after exposure to 0.1,1.0 and 10.0 ng/dm TBT for 8 d and to 1.0 and 10.0 ng/dm for 20 d, as compared with the matched control, while NADH cytochrorne b5 reductases and NADPH cytochrome c reductase activities were induced after exposure to 10.0 ng/dm^3 TBT for 2 d. The EROD activity in the 10 ng/dm^3 group,and the NADH cytochrome b5 reductases activities in 1.0 and 10.0 ng/dm groups, were significantly induced when transferred to clean recovery tanks for 7 d. The three enzymic activities in the clams exposed to TBT were recovered to the level corresponding to that of the control group after transfer to clean recovery tanks for 20 d. NADPH cytochrome c reductase activity in Meretrix meretrix seems to be more sensitive to exposure of TBT than that of the EROD and NADH cytochrome b5 reductases. The results suggest that induction and inhibition by TBT to the monooxygenase system enzymic activity in Meretrix meretrix would simultaneously exist. The enzymic activities were inhibited by exposure for a long time. The results suggest that inhibition of the monooxygenase system should be an indication of the exposure to environmentally relevant concentrations of TBT for a long time.     

动力定位系统与单波束测深仪在科考船定点作业中的应用

科考船定点作业时会受到海洋风、涌、浪、流等外界环境因素影响，导致工作效率降低，原位测量精度下降，甚至影响作业安全。动力定位系统 （DP） 具有自动定位功能，能够抵抗外界环境因素的影响，可实现科考船高精度定点控位。 单波束测深仪不仅可以测量水深，也可反映水下设备深度信息，可以起到辅助监控水下设备功能。本文在介绍定点作业施工现状与局限性的基础上，分析 DP 系统与单波束测深仪工作原理，以“向阳红 01”船为载体，在定点作业时开启 DP 系统与单波束测深仪，发现该方法可以提高科考船定点作业工作效率、原位测量精度并保障作业安全，可为其他科考船定点作业提供参考。     

THE INTERACTION OF COPPER WITH GOETHITE, HEMATITE AND AMORPHOROUS IRON OXIDE IN SEAWATER

1.This paper deals with a discovery of ours-a new type of isotherm in seawater systems, which cannot be represented by the adsorption isotherm equation available at present in marine chemistry. It has two characteristics as follows: (I) the non-linear relationship between the ion exchanged amount of particulate matter and the equilibrium concentration in the initial low concentration portion of the curve, showing instead an exponental function with slight concave upward. (2) the isotherm is made up of two “S shape” curves, which intersect each othernear the middle, with one “Knee” and two“Plateaus” at the intersection.     

东海海域海水中颗粒氨基酸(PAA)的组成与含量的分布调查研究

海水中的有机物的含量微少，约为0.5~2.0 C mg/L，但其种类复杂。其中以溶解有机物(DOM)为主，而颗粒有机物(POM)所占比例则更少。大洋海水中的POM主要来源于浮游生物等的死骸及其分解碎屑；另外还有海水中受风浪影响所进行的DOM-POM的动力平衡产物。近岸海水还受河流和大气带入的陆源尘影响。POM主要由颗粒氨基酸(PAA)组成。即由Asp、Glu、Thr、Ser、Ala、Gly、Leu、Ile、Val、Met、Cys、Tyr、Lys、His、Arg、Phe、Pro等17种氨基酸组成。它们是食碎动物的主要食物，构成海洋食物链的一环，与该水域生产力有直接联系。纪明侯等(1992)于1983年和1984年首次对青岛胶州湾海水中的颗粒氨基酸的组成和含量的变化及其季节变化进行了调查研究。并且在1982年和1984年前后对渤海湾、黄河口和长江口附近水域同样调查研究了表层海水中PAA的组成与含量分布。他们还于1981年7、8月和1982年7、8月乘“科学一号”考察船进一步研究了长江口外东中国海，包括福建省外海、济州岛附近海域和黑潮流域26°00′-33°00′N、123°00′-129°00′E范围内海域的20余个站位的表层海水和部分站位上不同水深中的PAA的组成和含量。兹将调查研究结果报道如下。     

吡喹酮和克螨特复配驱虫制剂对一些水生饵料生物的毒性

由吡喹酮和克螨特组成的复配制剂对小球藻、轮虫、蚤状溞、水蚯蚓和卤虫的毒性实验结果显示：复配制剂对轮虫的半致死浓度(LC50)为13．9mg／dm^3(24h)和3．03mg／dm^3(48h)．安全浓度(SC)为0．0432mg／dm^3；对蚤状溞的LC50为345．14mg／dm^3(24h)和17．5mg／dm^3(48h)，SC为0．0135mg／dm^3；对水蚯蚓的LC50为636．80mg／dm^3(24h)和79．07mg／dm^3(48h)，SC为0．366mg／dm^3；对卤虫无节幼虫的LC50为201．84mg／dm^3(24h)和8．91mg／dm^3(48h)．SC为0．00519mg／dm^3,复配制剂对水环境生物的破坏作用小．     

单体褶牡蛎(Crassostrea sp.)的研究

采用肾上腺素(EPI)和去甲肾上腺素(NE)诱导、颗粒固着基和先固着后脱基三种方法,对褶牡蛎的眼点幼虫进行处理,产生单体蛎苗。EPI和NE诱导的最适浓度为10~(-4)M,最适处理时间为3h,幼虫的不固着变态率分别达47.7％和46.6％。处理时间延长,变态率增加不明显。EPI和NE的诱导效果差别不明显,其诱导作用对稚贝的生长无明显副作用。颗粒固着基以贝壳粉为佳,最适颗粒的规格为幼虫的壳长。幼虫先固着后脱基而形成单体蛎苗,以可弯曲的灰色塑料板效果最好,固着的小贝可通过来回弯曲塑料板而从上面脱落下来,小贝长至1～2cm大小时,脱基最容易。     

胶州湾海水中颗粒有机碳含量的分布与变化

本文报道了胶州湾表层海水及C_3和D_3站中层和底层水中的颗粒有机碳(POC)的分布与变化规律。结果表明,胶州湾POC年平均浓度为155～410μg/L;平面分布有明显的规律性,近岸和河口一带的含量较高,湾中部较低,湾口诸站为最低;垂直分布有个别数值偏高或偏低现象,但没有明显的规律;季节变化较明显,初春较高,秋季较低。     

Determination of the Critical Value of Deflection for Embedded Cylindrical Structures

An embedded cylinder is a large-diameter cylinder embedded in a soil foundation. The state of failure of such an embedded cylindrical structure shows large deflection instead of slide and overturn of the traditional gravity type of structure placed on a rubble base or foundation base. The critical value of deflection of the embedded cylindrical structure, which is the maximum allowable deflection for stability calculation of the cylinder, is a vital control value. Through investigation on deflection and soil pressures on an embedded cylinder by model experiments, the variations of the angle of rotation θ of a cylinder with effective anti-overturning ratio η and moment MH of thrust are discussed. On the basis of experimental study, the critical value of deflection of the cylindrical structure is proposed in the paper. Meanwhile, the formulas for calculating deflection of cylinders are derived.     

渤海湾、黄河口和长江口海域海水中颗粒氨基酸含量的分布

海水中除含有大量无机物质外，还含有种类繁多，但含量很低的有机物质，其含量约为C 0.5-2.0mg/L。海水有机物质可分为溶解有机物质(DOM)和颗粒有机物质(POM)。前者为主要组分，后者所含比例很小。在大洋中POM的主要来源有:①浮游生物，尤其是浮游植物活体、死骸及其分解碎屑;②海水中进行的DOM-POM的动力平衡产物。近岸海水还受河流流入的陆地POM和大气带入的陆源尘埃的影响。POM主要由蛋白质、碳水化合物、脂类等组成。POM是食碎屑动物的良好食物，它构成海洋食物链的一环，与水域生物生产力有直接的联系。POM中蛋白质含量以颗粒氨基酸(PAA)表示。Daumas(1976)曾报道过沿岸海水中PAA含量的季节变异，纪明侯等(1992年)于1980-1981年首次对青岛胶州湾内PAA含量在不同站位和不同季节中的变化特征进行调査研究。作者等继而于1982年7月、1983年10月和1984年5、7、8月乘“金星二号”考察船前后首次研究了渤海湾、黄河口和长江口附近水域中颗粒氨基酸的组成与含量在不同站位中的分布。所得结果如下。