潮汐沉积韵律计算新方法及其应用 ——以四川江油地区中三叠统雷口坡组藻白云岩为例

提出一种新的高精确度的方法来计算沉积旋回韵律,即以样品断面光学灰度参数作为韵律沉积变化的替代指标,利用数字图像分析软件,通过最小二乘法、一阶差分法等数据处理后,定量计算沉积旋回韵律。对采自四川江油西南部中三叠统雷口坡组的纹层藻白云岩样品进行研究,观察样品抛光面和薄片,确定藻白云石具有明显的潮汐沉积韵律纹层。应用此方法计算得出研究区样品共有217个潮汐层偶对,层偶对最大厚度为4.2mm,通过人工计数对比证明了该方法的可行性。这种技术方法与传统方法相比更省时、更高效,可推广性高,同时也可应用于其它类型频段韵律旋回中。     

湖相碳酸盐岩烃源岩有机显微组分分类及其岩石学特征——以酒西盆地为例

酒西盆地主力烃源岩为下白垩统下沟组和赤金堡组暗色半深湖相-深湖相的白云质泥岩和泥质白云岩,是典型的湖相碳酸盐岩烃源岩。通过对酒西盆地下白垩统二百多块湖相碳酸盐岩烃源岩详细的有机岩石学研究,解剖了湖相碳酸盐岩烃源岩中有机质的赋存形式,揭示了藻纹层为有机质纹层的主要发育形式,其与富泥晶白云石纹层和富粘土纹层构成湖相碳酸盐岩烃源岩特有的"三元式纹层"沉积结构,系统总结了各显微组分的光性、成因、生烃性及分布规律,提出了以全岩和干酪根为基础的湖相碳酸盐岩有机显微组分分类方案,首次鉴定出对酒西盆地油气有贡献的主要生烃组分为腐泥无定形体、腐殖无定形体以及浮游藻类体、孢粉体、壳屑体五种有机显微组分。     

遗迹化石Zoophycos的系统修订与分类

三维形态结构复杂、分布广泛的Zoophycos一直是众多遗迹学家研究的热点.通过对1855年至2011年发表的具有详细形态描述并配有图版的动藻迹(Zoophycos)文献进行系统整理,基于详实的Zoophycos形态结构特征,系统修订了Zoophycos 属,将Zoophycos主要划分为9个种,即Zoopycos c...     

侧孢短芽孢杆菌对铜绿微囊藻藻际微生物群落的影响

【目的】探究铜绿微囊藻(Microcystis aeruginosa)藻际微生物群落组成以及与溶藻细菌侧孢短芽孢杆菌(Brevibacillus laterosporus)Bl-zj共培养对藻际微生物群落的影响。【方法】将侧孢短芽孢杆菌Bl-zj与铜绿微囊藻共培养4 d后(BL组),收集样品,以单独培养的铜绿微囊藻(BG组)为对照,提取样品总基因组DNA后进行16S rDNA扩增、构建文库、测序及生物信息学分析,比较BL组与BG组间藻际微生物群落的差异。【结果】测序平均获得高质量序列85390条,946个OUT(Operational Taxonomic Unit),BL组特有OTU 339个。物种组成分析显示,BG组微生物群落主要以微囊藻属(Microcystis)为主,其余相对丰度由高到低分别为假单胞菌属(Pseudomonas)、中慢生根瘤菌属(Mesorhizobium)、短芽孢杆菌属(Brevibacillus)和红杆菌属(Rhodobacter)。BL组微生物群落主要以短芽孢杆菌属(Brevibacillus)为主。BL组的微生物多样性相较于BG组显著下降(P<0.05)。经预测,BG组微生物功能集中在能量代谢与外源生物降解与代谢,BL组微生物功能集中在碳水化合物代谢和氨基酸代谢功能。【结论】侧孢短芽孢杆菌Bl-zj与铜绿微囊藻共培养,打破了铜绿微囊藻藻际微生物群落的平衡,与Bl-zj的溶藻进程有关。     

Protophyllocladoxylon jingyuanense sp.nov.,a Gymnospermous Wood of the Serpukhovian (Late Mississippian) from Gansu,Northwest China

<正>A new species of gymnospermous fossil wood,Protophyllocladoxylon jingyuanense sp.nov., is described from the lower part of the Tsingyuan Formation(Serpukhovian/Late Mississippian),near the coal mine of Ciyao,Gansu,northwestern China.The specimen is preserved only in silicified secondary xylem,while growth rings are absent.Pits in radial tracheidal walls are araucaroid in type, uniseriate,occasionally biseriate.Xylem rays are homogeneous,uniseriate,occasionally biseriate. Cross-field pits are simple,large,one to two in number.Axial parenchyma is absent.The anatomical characters and geographic distribution of Protophyllocladoxylon woods through geological ages are documented.Those species with axial parenchyma and without growth rings live in warm climate, whereas the species without axial parenchyma and with growth rings are present either in warm or cool climate.The ideal living climate for Protophyllocladoxylon woods is warm and wet.Our new species,as a fossil wood of Early Carboniferous,is likely the earliest known species of Protophyllocladoxylon.     

虾池微藻定向培育及其对养殖环境因子的影响

采用微藻三级扩大培养技术，将在对虾养殖池选育的波吉卵囊藻Oocysits borgei引入凡纳滨对虾Litopenaeus vannamei高位养殖池，并检测养殖环境中微藻群落结构、水质因子、对虾抗病力相关因子、对虾生长情况，研究了以微藻生态调控为主的对虾防病技术。结果表明：养殖前、中、后期，波吉卵囊藻平均生物量占浮游植物总生物量分别为98．02％、78．89％和45．12％，成为虾池中的绝对优势种；作为优势种的持续时间长达77d。波吉卵囊藻为主的微藻群落控制的水质较稳定，实验池氨氮和亚硝酸盐氮浓度较对照池低。对虾的血细胞数、溶菌（LSZ）活力、抗菌活力、超氧化物歧化酶（SOD）和酚氧化酶（PO）均显著高于对照池（P〈0．05）；血清蛋白含量差异不显著（P〉0．05）。实验池对虾生长速度显著高于对照池（P〈0．05）。可见，通过微藻的定向培育方法来优化虾池微藻群落结构，可改善养殖环境。     

卡帕藻的营养成分分析及综合比较

于1999年元月在海南陵水黎安港采集卡帕藻．应用HPLC、原子吸收等分析手段，系统测定其营养成分(主要包括蛋白质、氨基酸、脂肪、粗纤维、粗灰分、矿物质及微量元素等)，并与其它几种大型藻类比较．综合评价卡帕藻的营养价值。结果表明：卡帕藻蛋白质含量为6．20％，而粗脂肪和粗灰分较高，分别为2．88％和39．04％；卡帕藻中富含K、Na、Ca、Mg、P、Fe、Zn、Mn、Se等矿物元素，尤其是Se的含量远高于其它几种大型藻类。     

Cylindrotheca closterium Is a Species Complex as Was Evidenced by the Variations of rbcL Gene and SSU rDNA

The genus Cylindrotheca consists of a small group of marine diatoms with a few species described. Eleven isolates of diatoms identified as Cylindrotheca closterium morphologically were obtained from Jiaozhou Bay with their nuclear-encoded small-subunit ribosomal RNA （SSU rDNA） and chloroplast-encoded rbcL gene sequences determined in this study. Interestingly, very high sequence divergences of SSU rDNA and rbcL gene were found among these isolates, and numerous nucleotide variation of rbcL gene caused relatively few variation of deduced amino acid sequence. Phylogenetic analyses based on SSU rDNA and rbcL gene, respectively, grouped the isolates into 6 clades. Phylogenetic tree of SSU rDNA placed all the Cylindrotheca isolates together, separating them into two lineages clearly. LineageⅠ was composed of the eleven C. closterium isolates obtained in this study together with another C. closterium isolate, but some clades were not well supported. LineageⅠwas contained two C. closterium isolates and one C. fusiformis isolate. Phylogenetic analysis of rbcL gene also separated the Cylindrotheca isolates into two well-defined lineages. The eleven C. closterium isolates formed a lineage and all clades were supported strongly. Statistical comparisons of SSU rDNA indicated that the average distance within lineageⅠwas significantly higher than that of other microalgae species （P 〈0.01）. These results suggested the existence of cryptic species within C. closterium.