合浦珠母贝丝氨酸蛋白酶抑制因子基因pfser1克隆与表达

【目的】克隆合浦珠母贝(Pinctada fucata)丝氨酸蛋白酶抑制因子pfser1基因,探讨该基因的组织表达及其在天然免疫过程中的作用,以及与生物矿化过程的关系。【方法】通过RACE技术获得pfser1基因的全长,通过生物信息学分析其序列结构特征,利用实时荧光定量PCR方法检测pfser1基因在不同组织中的表达,检测健康合浦珠母贝在被大肠杆菌(Escherichia coli)MG1655刺激后和在贝壳损伤修复实验中pfser1基因表达量的变化。【结果】合浦珠母贝pfser1基因cDNA全长为1240 bp,包含1035 bp的开放阅读框(ORF),编码344个氨基酸,氨基酸序列的功能结构域含有丝氨酸蛋白酶抑制因子Serpin家族保守结构域。pfser1基因在合浦珠母贝各个组织中均有表达,在外套膜边缘膜中表达量最高;大肠杆菌MG1655刺激后,该基因表达量显著升高;在贝壳损伤修复过程中,pfser1基因表达先升高后受到抑制。【结论】pfser1基因所表达的蛋白参与了合浦珠母贝的天然免疫应答过程,并与生物矿化过程有一定关系。     

响应面法优化南极磷虾酶法制备DPP-IV抑制肽工艺条件的研究

采用动物蛋白水解酶酶解制备南极磷虾二肽基肽酶-IV(DPP-IV)抑制肽,以DPP-IV抑制率和可溶性蛋白含量为主要指标,考察温度、反应时间、pH、酶添加量对南极磷虾的酶解效果。在单因素的基础上,依据中心组合实验设计原理,运用4因素3水平的响应面分析法,建立动物蛋白水解酶酶解南极磷虾制备DPP-IV抑制肽的二次多项式数学模型,并以DPP-IV抑制率为响应值作响应面。结果表明:南极磷虾酶法制备DPP-IV抑制肽的最佳工艺参数为酶解时间3.85 h、温度45.02℃、pH值7.58、酶添加量237.55 U/g原料;在此条件下,酶解液DPP-IV抑制率的预测值为64.82%,验证值为64.78%,优化方法可行。     

引潮位展开的不同规格化形式及其转换

在引潮位展开过程中,为使大地系数的数值在不同阶次中保持相对稳定,对其进行规格化处理。从引潮位的基本理论公式出发,在分析缔合勒让德函数及其完全规格化的基础上,给出了引潮位展开中3类不同规格化(Doodson规格化、Cartwright & Tayler规格化、Hartmann & Wenzel规格化)公式的具体形式,得到3者之间的转换关系与转换系数。同时给出Doodson规格化中2~6阶规格化因子的具体数值,指出并改正Doodson、Roosbeek文献和IERS 2003、2010规范中的3处错误。     

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.     

土壤侵蚀危险度的计算与影响因子分析——以福建安溪红壤水土流失区为例

随着国家对生态环境的重视和水土保持科学的发展,有关土壤侵蚀危险性评价的研究越来越重要。本文对土壤侵蚀危险度评价的一般方法进行了改进。首先,分析了安溪县各强度等级的土壤侵蚀在高程、坡度、土地利用类型和土壤类型的面积分布情况,计算了不同高程带、不同坡度、不同土地利用类型和土壤类型的土壤侵蚀综合指数然后,以各影响因子的土壤侵蚀综合指数为权重,计算了不同侵蚀影响因子在安溪县各子流域的土壤侵蚀危险度,既避免了权重确定的主观性,又保证了土壤侵蚀危险度的计算适合当地的实际情况,从而明确了各子流域土壤侵蚀发生的可能程度最后,依据土壤侵蚀危险度和土壤侵蚀强度的分布规律,对整个研究区进行了水土保持分区,提出了治理方向。     

The selection of alkaline protease-producing yeasts fi＇om marine environments and evaluation of their bioactive peptide production

A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C, and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.     

抽水和建筑荷载双重作用下的地面沉降模型

依据含水层质量守恒原理,推导了非固结地层在建筑荷载作用下的地面沉降机理模型,并将其与Jacob假定下抽水引起的地面沉降弹性模型相比较,结果发现两种模型的机理表达式完全一致,仅区别于水动力原因或实质的不同.在同一坐标系中,根据线性叠加原理,抽水沉降漏斗和荷载沉降漏斗可能都落在地下水位降落漏斗内.假定两者为相互独立事件,给出了抽水和建筑荷载双重作用下的地面沉降模型,表明其如果以水位降深为制约因子,则可以对水动力实质完全不同的两种沉降过程进行线性叠加计算.     

有益微生物对水质因子的调控效应

随着我国水产养殖业的发展 ,集约化、半集约化的养虾模式随之迅猛发展 ,养殖规模不断扩大。在养殖过程中 ,因残存饵料腐烂 ,生物代谢 ,以及生物残体沉积 ,致使养殖水体理化环境和生态环境恶化 ,从而影响生物的生长、发育和行为活动。为改善养殖环境 ,促进水产养殖业健康可持续性地发展 ,近年来微生物技术在水产养殖业中得到了广泛应用[1,2 ] ,1) 。利用芽孢杆菌和光合细菌等有益微生物能调节和稳定养殖水体中的水质因子 ,分解、转化养殖水体的有机物的特性 ,达到改良、净化和营造养殖生态环境的效果 ,在生产上取得了明显的成效。为此 ,从 1 …     

北部湾墨西哥湾扇贝形态性状与闭壳肌质量的关系

为寻找墨西哥湾扇贝中对其闭壳肌重量影响最显著的形态学特征,在整个扇贝的生长过程中,每一个月测一次壳高(mm)、壳宽(mm)、铰合线长(mm)、体质量(g)和闭壳肌质量(g),每一轮的样本采集量为60,通过通径分析研究这些形态学特征对闭壳肌质量的影响。结果显示,根据各形态学特征对闭壳肌质量的直接的、间接的、总路径的贡献,发现体质量的影响是最显著的(p<0.01),其他的形态学特征如壳高、壳宽和铰合线长对于闭壳肌生长既不限制也不促进。