Identification of dissolved and particulate carbonyl compounds produced by marine harmful algal bloom species

Carbonyl compounds, especially polyunsaturated aldehydes (PUAs), are increasingly reported as teratogenic to grazers and deleterious to phytoplankton. While PUAs have been considered to be mainly produced by diatoms after cell wounding, little is known about whether microalgae other than diatoms, particularly harmful algal bloom (HAB)-forming species, produce carbonyl compounds. In this study, we analyzed the carbonyl compounds from eight common HAB-forming species ( Akashiwo sanguinea , Karenia mikimotoi , Karlodinium venefi cum , Margalefi dinium polykrikoides , Prorocentrum donghaiense , P . minimum , Scrippsiella trochoidea , and Heterosigma akashiwo ) using gas chromatography-mass spectrometry in full scan and selected ion monitoring (SIM) modes. Our results show a ubiquitous presence of carbonyl compounds in both dissolved and particulate forms in all species we examined. In the full scan mode, 133 chromatographic peaks were detected from 51 samples altogether. Both the varieties of carbonyl compounds and their quantities were algal species dependent, although most of the carbonyl compounds could not be fully identifi ed according to the mass spectral database only due to the unavailability of enough standards for all analytes. Aided with nine standards in SIM mode, we further identifi ed and quantifi ed all nine aldehydes (2-methyl-2-pentenal, trans-2-nonenal, cis-6-nonenal, 2,6-dimethyl-5-heptenal, trans-2-hexenal, trans-2- decenal, 2,4-heptadienal, trans-trans-2,4-octadienal, and trans-trans-2,4-decadienal). Four of these nine aldehydes were detected in particulate form, which confi rmed that trans-2-nonenal could be produced by K . mikimotoi , Karl . venefi cum , P . donghaiense , P . minimum , S . trochoidea , and H . akashiwo , 2,4-heptadienal and trans-trans-2,4-decadienal by A . sanguinea , M . polykrikoides , and S . trochoidea , and trans, trans-2,4- octadienal by S . trochoidea , respectively. We proved that some dinofl agellate and raphidophyte species could contribute to the pool of carbonyl compounds including PUAs in a marine ecosystem. Some carbonyl compounds, particularly those in high cell quota and/or dissolved concentration, may be related to fi shkilling or allelopathy which needs further identifi cation and quantifi cation.     

Application and comparison of two symptom-based eutrophication-assessment methods in Jiaozhou Bay,China

Increasing numbers of approaches to assess eutrophication, such as estuarine trophic status, the Oslo-Paris Commission Common Procedure, and the Water Framework Directive, focus on the symptoms of eutrophication. In China, however, nutrient index methods dominate the assessment of coastal waters. In this study, an integrated method that includes both water quality and ecological response was compared with the Northwest Pacifi c Action Plan (NOWPAP) Common Procedure. Observation data from Jiaozhou Bay, Shandong, China, were used in a comparison of the two methods in a trophic status study. Overall, both clearly revealed a high level of nutrient enrichment in the bay, indicated by high nutrient concentrations. Though the two methods diff ered in their methodological design in the assessment of the ecological eff ects of nutrient enrichment, they have acquired similar results: the integrated method suggested that the status was good, and the NOWPAP Common Procedure suggested that the status was low (indicating that the bay had no serious eutrophication problem). The introduction of fi lter feeders (shellfi sh aquaculture) into the bay on a reasonable scale may have been eff ective in mitigating eutrophic conditions, and perhaps explains the low ecological impacts there. Our results will be useful to ecosystem-based eutrophication management in the bay and in similar areas.     

Numerical simulation and preliminary analysis of typhoon waves during three typhoons in the Yellow Sea and East China Sea

In this study,typhoon waves generated during three typhoons(Damrey(1210),Fung-wong(1416),and Chan-hom(1509))in the Yellow Sea and East China Sea were simulated in a simulating waves nearshore(SWAN)model,and the wind forcing was constructed by combining reanalyzed wind data with a Holland typhoon wind model.Various parameters,such as the Holland fitting parameter(B)and the maximum wind radius?,were investigated in sensitivity experiments in the Holland model that affect the wind field construction.Six different formulations were considered and the parameters determined by comparing the simulated wind results with in-situ wind measurements.The key factors affecting wave growth and dissipation processes from deep to shallow waters were studied,including wind input,whitecapping,and bottom friction.Comparison with in-situ wave measurements suggested that the KOMEN scheme(wind input exponential growth and whitecapping energy dissipation)and the JONSWAP scheme(dissipation of bottom friction)resulted in good reproduction of the significant wave height of typhoon waves.A preliminary analysis of the wave characteristics in terms of wind-sea and swell wave revealed that swell waves dominated with the distance of R to the eye of the typhoon,while wind-sea prevailed in the outer region up to six to eight times the R values despite a clear misalignment between wind and waves.The results support the hypothesis that nonlinear wave-wave interactions may play a key role in the formation of wave characteristics.     

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.     

Verification and recalibration of HY-2A microwave radiometer brightness temperature

HY-2A is the first one of the Chinese HY-2 ocean satellite series carrying a microwave radiometer(RM)to measure sea surface temperature,sea surface wind speed,atmospheric water vapor,cloud liquid water content,and rain rate.We verified the RM level 1B brightness temperature(T B)to retrieve environmental parameters.In the verification,TB that simulated using the ocean-atmosphere radiative transfer model(RTM)was used as a reference.The total bias and total standard deviation(SD)of the RM level 1B TB,with reference to the RTM simulation,ranged-20.6-4.38 K and 0.7-2.93 K,respectively.We found that both the total bias and the total SD depend on the frequency and polarization,although the values for ascending and descending passes are different.In addition,substantial seasonal variation of the bias was found at all channels.The verification results indicate the RM has some problems regarding calibration,e.g.,correction of antenna spillover and antenna physical emission,especially for the 18.7-GHz channel.Based on error analyses,a statistical recalibration algorithm was designed and recalibration was performed for the RM level 1B TB.Validation of the recalibrated TB indicated that the quality of the recalibrated RM level 1B TB was improved significantly.The bias of the recalibrated T B at all channels was reduced to<0.4 K,seasonal variation was almost eradicated,and SD was diminished(i.e.,the SD of the 18.7-GHz channel was reduced by more than 0.5K).     

New photometric investigation of the low-mass-ratio contact binary star V1853 Orionis

Four-color charge-coupled device(CCD) light curves in the B, V, Rc and I c bands of the totaleclipsing binary system V1853 Orionis(V1853 Ori) are presented. By comparing our light curves with those published by previous investigators, it is determined that the O'Connell effect on the light curves has disappeared. By analyzing those multi-color light curves with the Wilson-Devinney code(W-D code),it is discovered that V1853 Ori is an A-type intermediate-contact binary with a degree of contact factor of f = 33.3%(3.7%) and a mass ratio of q = 0.1896(0.0013). Combining our 10 newly determined times of light minima together with others published in the literature, the period changes of the system are investigated. We found that the general trend of the observed minus calculated(O-C) curve shows a downward parabolic variation that corresponds to a long-term decrease in the orbital period with a rate of d P/dt =-1.96(0.46)×10^-7 d yr^-1. The long-term period decrease could be explained by mass transfer from the more-massive component to the less-massive one. By combining our photometric solutions with data from Gaia DR_2, absolute parameters were derived as M_1 = 1.20 M⊙, M_2 = 0.23 M⊙, R_1 = 1.36 R⊙and R_2 = 0.66 R⊙. The long-term period decrease and intermediate-contact configuration suggest that V1853 Ori will evolve into a high fill-out overcontact binary.     

Analysis of Spatio-temporal Land Surface Temperature and Normalized Difference Vegetation Index Changes in the Andassa Watershed,Blue Nile Basin,Ethiopia

Analysis of the nexus between vegetation dynamics and climatic parameters like surface temperature is essential in environmental and ecological studies and for monitoring of the natural resources. This study explored the spatio-temporal distribution of land surface temperature (LST) and Normalized Difference Vegetation Index (NDVI) and the relationship between them in the Andassa watershed from 1986 to 2016 periods using Landsat data. Monthly average air temperature data of three meteorological sites were used for validating the results. The findings of the study showed that the LST of the Andassa watershed has increased during the study periods. Overall, average LST has been rising with an increasing rate of 0.081°C per year. Other results of this study also showed that there has been a dynamic change in vegetation cover of the watershed in all seasons. There was also a negative correlation between LST and NDVI in all the studied years. From this study we can understand that there has been degradation of vegetation and intensification of LST from 1986 to 2016.     

Wave tank and bench-top control testing of a wave energy converter

An increasing number of experiments are being conducted to study the design and performance of wave energy converters. Often in these tests, a real-time realization of prospective control algorithms is applied in order to assess and optimize energy absorption as well as other factors. This paper details the design and execution of an experiment for evaluating the capability of a model-scale WEC to execute basic control algorithms. Model-scale hardware, system, and experimental design are considered, with a focus on providing an experimental setup capable of meeting the dynamic requirements of a control system. To more efficiently execute such tests, a dry bench testing method is proposed and utilized to allow for controller tuning and to give an initial assessment of controller performance; this is followed by wave tank testing. The trends from the dry bench test and wave tank test results show good agreement with theory and confirm the ability of a relatively simple feedback controller to substantially improve energy absorption. Additionally, the dry bench testing approach is shown to be an effective and efficient means of designing and testing both controllers and actuator systems for wave energy converters.     

Obtaining the distribution of quiescent periods directly from the power spectral densities of Sea waves

There is a growing practical interest in the ability to increase the sea states at which marine operations can be safely undertaken by exploiting the quiescent periods that are well known to exist under a wide range of sea conditions. While the actual prediction of quiescent periods at sea for the control of operations is a deterministic process, the long term planning of future maritime tasks that rely on these quiescent periods is a statistical process involving the anticipated quiescence properties of the forecasted sea conditions in the geographical region of interest. It is in principle possible to obtain such data in tabular form either large scale simulation or from field data. However, such simulations are computationally intensive and libraries of appropriate field data are not common. Thus, it is clearly attractive to develop techniques that exploit standard wave spectral models for describing the quiescence statistics directly from such spectra. The present study focuses upon such techniques and is a first step towards the production of a computationally low-cost quiescence prediction tool and compares its efficacy against simulations. Two significant properties emerge for a large class of wave spectral models that encompasses the ubiquitous Neumann and Pierson Moskowitz or Bretschneider forms. Firstly, the auto-correlation function of the wave profile that are required to produce the quiescence property can be obtained analytically in terms of standard special functions. This considerably reduces the computational cost making desktop computer-based planning tools a reality. Secondly, for each class of these parametric spectra, the probability of a given number of consecutive wave heights (normalised to the significant wave heights) less than some critical value is in fact independent of absolute wave height. Thus, for a broad class of practically interesting wave spectra all that is required to obtain the statistical distribution of the quiescent periods is simple rescaling.     

基于支持向量机的京津冀城市群热环境时空形态模拟

城市群热环境作为区域生态重要组成部分,已成为近年来的研究热点。而如何选择针对城市群这种复杂地地貌特征的热环境量化工具一直是亟待解决的技术难点,基于此本研究提出了一种解决多样本、非线性、非平稳及高维函数拟合的计算方法,并建立了基于支持向量机(SVM)的京津冀城市群热环境曲面模型来揭示城市群热环境的时空形态变化。研究结果表明：① SVM模型在刻画多核心、多种土地利用类型城市群热环境的空间分布方面具有理论与实践可行性,能够根据热环境的整体空间布局通过高斯核函数进行局部优化差值,最大限度减少缺省值对模型拟合结果的影响。相比于对照方法可以模拟出更高精度的复杂地貌特征城市群热岛空间分布格局;② 在SVM模型曲面拟合的过程中,拟合精度和拟合时间是衡量拟合结果的重要指标,而原始影像的分辨率则是影响该指标的决定性因素;③ 2003-2013年区域内北京市与天津市的城市热岛效应变化最为明显,热岛面积分别增加7091 km²与4196 km²,空间上呈现出逐年接近连片发展趋势,热岛重心移动轨迹具有明显的时空分异性。北京城市热岛特征为东南部地区异速增长,西部地区缓慢增长;天津城市热岛特征为以城市中心为圆心向周围扩展。本研究进一步丰富了城市群热环境评测的定量方法,可以在实践上对城市群的城市规划、城市建设、环境保护和区域可持续发展等提供定量化、可视化的决策支持。