Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

Preliminary seismic anisotropy in the upper crust of the south segment of Xiaojiang faults and its tectonic implications

The Xiaojiang faults,striking north-to-south(NS),and the Honghe faults,striking north-to-west(NW),are first-order block boundaries that intersect to form a concentrated stress zone at an acute angle in the southern part of the Sichuan-Yunnan rhombic block(SYB).It is also a crucial zone for material escaping from the Tibetan Plateau(TP)due to the collision between the Indian Plate and the Eurasian Plate.In December 2017,the Institute of Earthquake Forecasting of the China Earthquake Administration(CEA)deployed a linear temporary seismic broadband array,the Honghe-Xiaojiang temporary Seismic Array(HX Array),across first-order block boundaries in the southern SYB.By using the waveform data of small earthquakes recorded by stations in the HX Array across Xiaojiang faults from 2017 to 2019,and by permanent seismic stations of the China National Earthquake Networks from 2012 to 2019,this paper adopts the systematic analysis method of shear-wave splitting(SWS),SAM method,to obtain preliminary results for seismic anisotropy in the upper crust.The study area can be divided into two subzones according to the spatial distribution of the directions of polarization of the fast shear-wave(PFS)at the stations:the northern zone(zone A,where the HX Array is located)and the southern zone(zone B,to the south of the HX Array).The results show that the directions of the PFS at stations in zone A were highly consistent,dominant in the NE direction,correlated with the in-situ principal compressive stress,and were seemingly unaffected by the Xiaojiang faults.The directions of the PFS as recorded at stations in zone B were more complicated,and were dominant in the NS direction parallel to that of the regional principal compressive stress.This suggests the joint influence of complex tectonics and regional stress in this narrow wedge area.By referring to the azimuthal anisotropy derived from seismic ambient noise in the southeast margin of the TP,the NS direction of the PFS in the middle and lower crust,and its EW direction in the upper mantle,this paper concludes that azimuthal anisotropy in the upper crust differed from that in the lower crust in the south segment of Xiaojiang faults,at least beneath the observation area,and azimuthal anisotropy in the crust was different from that in the upper mantle.The results support the pattern of deformation of ductile flow in the lower crust,and the decoupling between the upper and lower crusts as well as that between the crust and the mantle in the study area.The crustal directions of the PFS appeared to be independent of the Xiaojiang faults,suggesting that the influence of the South China block on the SYB passed through the Xiaojiang faults to the Yimen region.The results of this study indicate that anisotropic studies based on data on the dense temporary seismic array can yield clearer tectonic information,and reveal the complex spatial distribution of stress and deformation in the upper crust of the south segment of Xiaojiang faults.     

大同地区3次地震序列构造应力张量变化特征的研究

利用1989~2002年间3次大同地震序列中共计700多次中小地震的震源机制解资料,应用Gephart(1990)的应力张量反演方法研究了这3次地震序列的构造应力张量的总体变化特征和时序变化特征,研究发现3次主震发生前震源及附近地区的构造应力作用较强,主震发生前后,应力方向存在细节差异,但是最大主压应力方向与华北地区的构造应力场方向基本一致,只有1999年震前阶段的最大主压应力方向为226°(SW向),分析认为这可能是华北地区构造应力场与大同地区局部构造应力场相互作用的结果。     

围压-孔压改变条件下致密砂岩及泥页岩泊松比变化特征及机制

在围压(外压)或孔压(内压)发生变化的条件下,致密砂岩及泥页岩泊松比的变化特征及机制仍有待厘清.本研究从Terzaghi有效应力理论和国内学者提出的新有效应力概念出发,基于松辽盆地高台子组致密砂岩、青山口组泥页岩三向动、静态泊松比测定结果,剖析了两类岩石泊松比的变化特征及机制.岩石样品三向泊松比变化曲线的分布呈现显著的...     

氨氮胁迫对中华绒螯蟹(Eriocheir sinensis) 免疫功能的影响

在实验室条件下构建不同氨氮浓度环境和养殖时间的组间差异,研究氨氮胁迫对中华绒螯蟹免疫功能的影响。结果表明,中华绒螯蟹在NH 4-N3.0mg/L10天或1.0mg/L20天时,其血淋巴血细胞密度极显著地低于对照组(P<0.01)。NH4 -N2.0mg/L和3.0mg/L无论10天或20天,血细胞吞噬百分率均分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N3.0mg/L10天或2.0mg/L20天,血细胞吞噬指数显著低于对照组(P<0.05)。NH4 -N1.0mg/L和2.0mg/L分别20天时,血清溶菌酶(LSZ)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N1.0—5.0mg/L10天时,血清酚氧化酶(PO)活力均极显著(P<0.01)下降;但NH4 -N1.0—4.0mg/L20天时,血清PO活力水平均比10天时有所上升。NH4 -N3.0—4.0mg/L10天或1.0—2.0mg/L20天,以及5.0mg/L10天或3.0—5.0mg/L20天时,超氧化物歧化酶(SOD)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。上述结果表明,随着氨氮胁迫浓度的增加和胁迫时间的延长,会引起中华绒螯蟹血细胞数量、血细胞吞噬能力、溶菌酶活力、酚氧化酶活力和超氧化物歧化酶活力等的逐渐下降,使机体非特异性免疫防御系统遭到损伤,同时机体清除自由基的能力下降,机体细胞和组织受到伤害甚至出现死亡。     

黄河三角洲潮滩剖面特征

利用验潮、潮滩水准测量、沉积物分析和遥感资料,分析了黄河三角洲不同岸段潮滩的特征。水准测量表明,侵蚀潮滩剖面形态为下凹,而淤积潮滩则上凸,由高潮线至低潮线,潮滩沉积物均变粗,这种变粗趋势在北部侵蚀岸段和河口侧缘明显,在河口南侧稳定海岸则不明显。潮滩沉积物含水量为12.2%~32.2%,平均为21%,海滩表层沉积物不排水剪切强度在0.1~0.35 kg/cm2之间。受局限岸滩的平面形态和剖面形态受到周围大坝地形影响,具有和砂质海滩类似的形态,显示波浪对该潮滩的作用强烈,根据剖面测量结果与遥感图像,发现河口北侧有一个小的冲积扇。     

云南地区地壳介质各向异性——快剪切波偏振特性

通过对云南遥测地震台网2000年1月1日——2003年12月31日4年资料的分析, 使用剪切波分裂SAM综合分析方法,获得了云南地区10个数字地震台站的快剪切波偏振结果. 结果表明, 云南地区大部分台站的快剪切波偏振优势方向主要为近N——S或NNW方向; 位于活动断裂上的台站的快剪切波偏振优势方向与活动断裂的走向一致;与GPS主压应变方向一致,与区域主压应力方向基本一致;少数台站的快剪切波偏振较为复杂,或与活动断裂的走向及GPS主压应变方向不一致. 这样的台站总是位于几个断裂的交会处,反映了复杂的断裂背景和复杂的应力分布特征. 快剪切波偏振优势方向代表了原地最大主压应力方向,受到区域应力场和断裂分布等多种因素的控制.      

位移测量反演分析的解算模型与应用

利用现场位移测量资料反演分析是进行洞室围岩稳定分析和了解岩体介质中洞室的力学性状与设计参数的一种新手段。阐述了位移测量反演分析的基本思路、解算模型、成果检验等,并用计算实例说明了其有效性。     

有机质热演化过程中地层压力的作用与影响

压力对有机质演化的影响长期以来争论不断,共有3种不同意见：压力对有机质演化没有影响,压力促进有机质的演化,压力抑制有机质的演化。通过对前人所做实验的实验设备、实验样品及实验结果的分析对比发现：不同实验结果主要是由于不同实验条件引起的,特别是实验系统和加压方式的不同,对实验结果影响最大,所代表的地质意义也不同。综合分析结果表明,当压力的增加表现为沉积有机质受到的有效应力增加时可以促进有机质生烃;当压力的增加表现为孔隙流体压力增加时,有机质生烃过程将受到抑制;另外,这两种情况都可能使干酪根的镜质组反射率降低,易使人得出压力增加抑制有机质演化的结论。进一步对压力影响有机质演化的成因机制进行了分析,发现在有机质演化的不同阶段因异常压力的增压机制不同,压力在有机质演化过程中所表现出来的作用也有差异。