白东构造带成因分析

位于渤海湾盆地黄骅坳陷北部的白东构造带,受新构造运动影响形成了整体东西向"S"型展布,堑心组合内断层成弧形交错、南北对掉的"包心菜"状构造。笔者以局部构造变形特征分析为手段,从各层位上堑心构造组合的特殊现象分析出发,认为本区自第三纪以来是在右旋走滑拉分背景下伸展走滑作用的结果。     

枣庄市台儿庄区小龚庄岩溶地下水水源地允许开采量计算评价

科学有效的地下水资源评价是可持续开发地下水资源的关键,其中地下水资源可开采量的评价是地下水资源评价的核心内容。该文在对枣庄市台儿庄区小龚庄水源地水文地质条件、岩溶塌陷发育情况等进行详细分析的基础上,采用开采试验法对小龚庄水源地岩溶地下水允许开采量进行了计算,计算结果为11354m~3/d。根据已发生岩溶塌陷情况,设定岩溶塌陷预警水位,利用非稳定流Theis公式计算了安全开采量范围在11187～13150m~3/d之间,对开采试验法计算结果进一步进行评价。根据评价结果最终确定以11354m~3/d作为小龚庄水源地的允许开采量是可靠的,可以保证水源地最大限度的开采地下水资源,同时又保证水源地运行时不易引发岩溶塌陷的环境地质问题。该方法为同类型水源地地下水允许开采量的计算评价提供了一定的借鉴。     

Spatial Distribution Assessment and Source Apportionment of Land-Based Pollutant Yield from Qingdao,China

The spatial distribution of chemical oxygen demand(COD) and total nitrogen(TN) yield from Qingdao are studied by comparing pollutant yield amount, densities and spatial aggregation(Getis-Ord indexes) among the land-based pollutant source regions(PSRs) entering the three sub-seas(i.e. the Jiaozhou Bay(JZB), other coastal area in the Yellow Sea(OCAYS) and Laizhou Bay(LZB), respectively). Industrial composition of the loads are also studied by comparing pollutant yield among the sources of agriculture, rural domesticity, industry, urban domesticity and service, and calculation of Gini coefficient. Results show that spatial distribution of COD and TN yield from Qingdao are extremely unbalanced. The JZB, with less than 3% of the total coastal sea area of Qingdao, received 62% COD load and 65% TN yield from Qingdao, while the OCAYS, with more than 97% area, only received 23% COD and 20% TN, which consist with the much worsen water quality of JZB than that of OCAYS. On the other hand, the source apportionment of COD and TN loads in the PSRs entering JZB and the OCAYS was similar. The agricultural and domestic sources with high pollution intensity account for more than 80%, while the industrial and service sources with low pollution intensity account for less than 20%. While Gini coefficients, COD 0.81 and TN 0.84 which are much higher than the ‘imbalance' threshold of 0.4, show the uneven industrial structure of Qingdao. These results may be useful in the determination of land-based pollution total amount control at the PSR level.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

Temporal and Spatial Dynamics Analysis of Grassland Ecosystem Pressure in Kazakhstan

Affected by climate change and policy factors, Kazakhstan is the country with the most severe ecological degradation and grassland conflicts in Central Asia. Therefore, studying the state of grassland carrying resources in Kazakhstan is particularly important for understanding the responses of grassland ecosystems to climate change and human activities. Based on Kazakhstan's remote sensing data and animal husbandry statistics, this study analyzes the patterns of changes in grassland ecosystems in Kazakhstan based on the supply and consumption of these ecosystems. The results show that: 1) From 2003 to 2017, the number of livestock raised in Kazakhstan showed a trend of sustained and steady growth. Due to freezing damage, the scale of livestock farming decreased in 2011, but a spatial difference in the livestock farming structure was not obvious. 2) The fluctuation of grassland supply in Kazakhstan has increased, while the consumption due to animal husbandry has also continued to increase, resulting in an increasing pressure on the grassland carrying capacity. 3) Between 2003 and 2017, the overall grassland carrying status of Kazakhstan have been abundant, but the grassland carrying pressure index has shown a steadily increasing trend, the grassland carrying pressure is growing, and it is mainly determined by grassland productivity. The greater pressure in lower Kyzylorda state, the southern Kazakhstan state of the cultivated land and the northern Kazakhstan state has gradually expanded to include the agro-pastoral zone and the semi-desert zone.     

三疣梭子蟹几丁质酶基因(PtCht6)的克隆及其在免疫中的功能分析

几丁质酶(chitinase)在甲壳动物的蜕皮、消化和免疫等很多生物学过程中发挥重要功能,为深入探讨几丁质酶的免疫防御机制,本实验利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)PtCht6基因。该基因cDNA全长2736bp,开放阅读框(ORF)2103bp,编码700个氨基酸,具有几丁质酶GH18家族典型特征。利用实时荧光定量技术分析PtCht6在三疣梭子蟹不同组织、不同蜕皮阶段、不同病原感染以及低盐胁迫(11)下的表达特征,结果显示:PtCht6在各组织中均有表达且在肝胰腺中的表达量最高;在不同蜕皮时期的肝胰腺中,其表达量由蜕皮后期(A/B)、蜕皮间期(C)、蜕皮前期(D)依次递减(P0.05);人工感染WSSV和副溶血弧菌后.PtCht6的表达量在肝胰腺中分别于12h、72h达到最大值,在血细胞中均于12h达到最大值,且相较于对照组,整体显著上调表达(P0.05);低盐胁迫能够显著抑制该基因的表达,最高抑制73倍(P0.05)。同时发现,低盐环境下感染WSSV后,该基因达到峰值的时间明显延迟(至少延迟12h.P0.05)。该研究结果预示PtCht6作为免疫因子参与三疣梭子蟹的病原防御,且低盐胁迫在一定程度上抑制了该基因的免疫功能。     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

Current-induced scour beneath initially elevated subsea pipelines

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.     

Effects of wave directionality on extreme response for a long end-anchored floating bridge

Reliable design codes are of great importance when constructing new civil engineering concepts such as floating bridges. Previously only a scarce number of floating bridges have been built in rough wave conditions and only limited knowledge of the extreme environmental conditions and the associated extreme response exists. To form a better design basis an increased understanding of the sensitivity in the structural response towards changes in short-crested sea parameters is needed. Furthermore, acquiring the necessary accuracy in simulated extreme response is often a computationally expensive endeavour and the number of simulations needed is often based on experience. The present study investigates the wave-induced short-term extreme response of a simplified end-anchored floating bridge concept for several wave environments with a return period of 100 years. The study includes convergence of the coefficient of variation for the extreme response for different realization lengths as well as number of realizations. The sensitivity in the structural response towards different main wave directions and spreading exponents is investigated and includes both transverse and vertical displacement response spectra and extreme Von Mises stress in the bridge girder cross-section. The extreme response is based on an accuracy of 2% in the coefficient of variation equivalent to 40 3-h realizations and a low sensitivity in the response is found for natural occurring spreading exponents and for main wave directions within 15° from beam sea.