In Situ Measurement of Pinna nobilis Shells for Age and Growth Studies: A New Device

Abstract. Pinna nobilis Linnaeus 1758 is an endemic bivalve mollusc in the Mediterranean Sea, where it inhabits seagrass meadows, especially Posidonia oceanica (L.) Delile. It is the largest bivalve in the Mediterranean, reaching lengths up to 120 cm. In its natural habitat, P. nobilis lives with the anterior part of the valve buried in the seabed, attached to Posidonia rhizomes by byssus threads.
This habit makes it impossible to measure its total length directly in situ. As the only way to determine the individual age is the relationship between age and total length, several equations have been proposed to estimate total length by relating it to the unburied parts of the shell. Such measurements are essential to ecological studies that consider age, growth, and population dynamics, and that evaluate the environmental factors that affect this species.
Accurately estimating total length depends on the accuracy and precision of the method employed to measure the unburied shell parts. In this paper, we point out the lack of precision of the instruments and methods used until now; we also demonstrate the reason for this imprecision. A new device to measure unburied parts of Pinna nobilis with a precision comparable to that obtained when measuring extracted valves is described. This device is unaffected by substratum type and reduces measurement time. The latter is a very important feature, because these procedures are usually performed whilst SCUBA diving. Finally, a growth equation has been fitted to the measurements obtained with the new device from a population located in Moraira (Alicante, western Mediterranean).     

A Comparison of Simulated Particle Fluxes Using NEMURO and Other Ecosystem Models in the Western North Pacific

JGOFS has revealed the importance of marine biological activity to the global carbon cycle. Ecological models are valuable tools for improving our understanding of biogeochemical cycles. Through a series of workshops, the North Pacific Marine Science Organization (PICES) developed NEMURO (North Pacific Ecosystem Model Understanding Regional Oceanography) a model, specifically designed to simulate the lower trophic ecosystem in the North Pacific Ocean. Its ability to simulate vertical fluxes generated by biological activities has not yet been validated. Here compare NEMURO with several other lower trophic level models of the northern North Pacific. The different ecosystem models are each embedded in a common three-dimensional physical model, and the simulated vertical flux of POM and the biomass of phytoplankton are compared. The models compared are: (1) NEMURO, (2) the Kishi and Nakata Model (Kishi et al., 1981), (3) KKYS (Kawamiya et al., 1995, 2000a, 2000b), and (4) the Denman model (Denman and Peña, 2002). With simple NPZD models, it is difficult to describe the production of POM (Particulate Organic Matter) and hence the simulations of vertical flux are poor. However, if the parameters are properly defined, the primary production can be well reproduced, even though none of models we used here includes iron limitation effects. On the whole, NEMURO gave a satisfactory simulation of the vertical flux of POM in the northern North Pacific.     

Air-Sea Gas Transfer Velocity in Stormy Winter Estimated from Radon Deficiency

Thirteen vertical profiles of ²²⁶Ra and ²²²Rn in the near-surface water were obtained in the western North Pacific in winter, and the gas transfer velocities across the air-sea interface were estimated. The transfer velocities found by applying a steady state model varied widely from 2.1 to 30.2 m day⁻¹ with a mean of 9.4 m day⁻¹. The mean value is almost 5 times higher than that in summer in other oceans, and the maximum value is a record high for world oceans. This is partly due to the inadequacy of the steady state model, which overestimates when stronger winds blow in more recent days than the ²²²Rn half-life of about 4 days. In fact, a strong low pressure zone passed through the station about 2 days earlier, which was one of the low pressure zones that with a period of develop once a week or so in the northwestern North Pacific in winter. Instead of steady-state removal, if half of the radon removal occurred sporadically every 7 days, and the last removal took place two days before the observation, the transfer velocity would be 26 m day⁻¹. Our mean transfer velocity, which is less than 20% different from the steady state value including both overestimated and underestimated values, 9.4 ± 4.8 m day⁻¹, seems to represent the mean state of this region in winter. This suggests that the gas exchange fluxes under extremely rough conditions in the open ocean are larger than those estimated by using a transfer velocity equation with a linear or quadratic relationship with wind speed. This revised version was published online in July 2006 with corrections to the Cover Date.     

Outflow of the Intermediate Oyashio Water from Sagami Bay to the Shikoku Basin

Since the Intermediate Oyashio Water (IOW) gradually accumulates in Sagami Bay, it can reasonably be supposed that the IOW also flows out from Sagami Bay, even though it may be altered by mixing with other waters. We have occasionally observed a water less than 34.2 psu with a potential density of 26.8 at the southeastern area off Izu Peninsula in July 1993 by the training vessel Seisui-maru of Mie University. Observational data supplied by the Japan Meteorological Agency and the Kanagawa Prefectural Fisheries Experimental Station show that the IOW of less than 34.1 psu was observed at northern stations of the line PT (KJ) off the Boso Peninsula and to the east of Oshima in the late spring 1993. Based upon these observations, it is concluded that the IOW flows out from Sagami Bay into the Shikoku Basin along southeastern area off the Izu Peninsula. The less saline water (<34.2 psu) was also observed to the west of Miyake-jima during the same cruise, and the westward intrusion of IOW from south of the Boso Peninsula to the Shikoku Basin through the gate area of the Kuroshio path over the Izu Ridge was detected. This event indicated that the IOW branched south of the Boso Peninsula and flowed into Sagami Bay and/or into the gate area over the Izu Ridge. The southward intrusion of IOW into the south of the Boso Peninsula is discussed in relation to the latitudinal location of the main axes of the Kuroshio and the Oyashio. This revised version was published online in July 2006 with corrections to the Cover Date.     

North Pacific Subtropical Mode Waters Observed in Long XBT Cross Sections along 32.5°N Line

The S/V Shoyo, of the Hydrographic Department, Japan Coast Guard, has conducted high-density expendable bathythermograph (XBT) measurements along the 32.5°N line in the North Pacific every year from 1990 to 1993 as a part of the Japanese-World Ocean Circulation Experiment (WOCE). These XBT data are analyzed here, focusing on year-to-year variations of the inventory and core layer temperature (CLT) of the North Pacific subtropical mode water (NPSTMW). Large year-to-year changes are found in the NPSTMW CLTs estimated in longitudes between 140°E and 160°E. CLT values were found of 17.4°C in 1990, 17.1°C in 1991, 17.3°C in 1992 and 17.6°C in 1993. Inspection of the wintertime westerlies over the formation area and sea surface temperature distribution revealed that this change in CLT can be qualitatively attributed to the strength of atmospheric cooling in the formation area in the previous winter. Although a large year-to-year variation of NPSTMW inventory was also found, it is hard to state any relationship between CLT and atmospheric forcing. There is a possibility that different observational seasons may affect the inventory. It has also been found that the thermocline depth in 1991 was shallower in the sea area east of 180° than in 1992 and 1993. Associated with this change, the North Pacific central mode water (NPCMW), characterized by thermostad with temperatures ranging from 14°C to 11°C, appears in the sea area east of 180° in the 1992 and 1993 cross sections. The 1993 cross section, which ranged from the Japanese coast to the west coast of North America, possessed another thermostad in the surface layer, with a temperature of about 17°C in the eastern part of the cross section, off California. This revised version was published online in August 2006 with corrections to the Cover Date.     

Physiological and behavioral responses of Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) to hypersaline conditions at a brine pool cold seep

Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) are two of the most abundant invertebrates associated with cold‐seep mussel beds in the Gulf of Mexico. At the methane seep known as Brine Pool NR‐1 (27 °43.415 N, 91 °16.756 W; 650 m depth), which is surrounded by a broad band of mussels (Bathymodiolus childressi), these species have distinctly different patterns of abundance, with the gastropod being found mostly at the outer edge of the mussel bed (average density in November 2003: 817 individuals·m⁻² in outer zone, 20·m⁻² in inner zone) and the polychaete being found almost exclusively near the inner edge (average density in November 2003: 3155 individuals·m⁻² in inner zone, 0·m⁻² in outer zone), adjacent to the brine pool itself. The salinity of the brine pool exceeds 120, so we hypothesized that M. dendrobranchiata should be more tolerant of high salinities than B. naticoidea. The opposite proved to be true. The gastropods were capable of withstanding salinities at least as high as 85, whereas the polychaetes died at salinities higher than 75. Both species were osmoconformers over the range of salinities (35–75) tested. Behavioral responses of B. naticoidea to salinities of 50, 60, and 70 were investigated in inverted vertical haloclines. Gastropods generally did not enter water of salinity greater than 60, but tolerated short periods at 60. Behavioral avoidance of brine should limit the vertical distribution of B. naticoidea in the inner zone to the top 2.5–5 cm of the mussel bed. Behavior is also a likely (though unproven) mechanism for controlling horizontal distribution of this species across the mussel bed. Methanoaricia dendrobranchiata can tolerate short excursions into the brine, but probably avoids hypersaline conditions by aggregating on the tops of the mussels.     

Further evidence for non-reemergence of winter SST anomalies in the North Pacific eastern subtropical mode water area

Following our previous study (Sugimoto and Hanawa, 2005b), we further investigate the reason why reemergence of winter sea surface temperature anomalies does not occur in the North Pacific eastern subtropical mode water (NPESTMW) area, despite its occurrence in the North Pacific subtropical mode water and North Pacific central mode water areas. We use vertical temperature and salinity profiles of the World Ocean Circulation Experiment Hydrographic Program and Argo floats with high vertical and temporal resolution, together with heat flux data through the sea surface. We point out first that one of the causes for non-occurrence of reemergence is that the thickness of NPESTMW is very thin. In addition to this basic cause, two major reasons are found: a vigorous mixing in the lower portion of NPESTMW and less heat input from the atmosphere in the warming season. Since, in the lower portion of NPESTMW and deeper, the stratification is favorable for salt-finger type convection to occur compared with the other mode water areas, vigorous mixing takes place. This is confirmed by both a large Turner Angle there and the existence of staircase structures in vertical temperature and salinity profiles. From the viewpoint of heat input, the NPESTMW area gradually gains heat in the warming season compared with other mode water areas. As a result, NPESTMW cannot be capped so quickly by the shallow summer mixed layer, and water properties of NPESTMW are to be gradually modified, even in the upper portion.     

Active spreading and hydrothermalism in North Fiji Basin (SW Pacific). Results of Japanese French Cruise Kaiyo 87

The aim of the Japanese-French Kaiyo 87 cruise was the study of the spreading axis in the North Fiji Basin (SW Pacific). A Seabeam and geophysical survey allowed us to define the detailed structure of the active NS spreading axis between 16° and 22° S and its relationships with the left lateral motion of the North Fiji Fracture Zone. Between 21° S and 18°10′ S, the spreading axis trends NS. From 18°10 S to 16°40 S the orientation of the spreading axis changes from NS to 015°. North of 16°40′ S the spreading axis trends 160°. These two 015° and 160° branches converge with the left lateral North Fiji fracture zone around 16°40′ S to define an RRFZ triple junction. Water sampling, dredging and photo TV deep towing give new information concerning the hydrothermal activity along the spreading axis. The discovery of hydrothermal deposits associated with living communities confirms this activity.     

Evidence for the grazing hypothesis: Grazing reduces phytoplankton responses of the HNLC ecosystem to iron enrichment in the western subarctic pacific (SEEDS II)

A mesoscale iron-enrichment study (SEEDS II) was carried out in the western subarctic Pacific in the summer of 2004. The iron patch was traced for 26 days, which included observations of the development and the decline of the bloom by mapping with sulfur hexafluoride. The experiment was conducted at almost the same location and the same season as SEEDS (previous iron-enrichment experiment). However, the results were very different between SEEDS and SEEDS II. A high accumulation of phytoplankton biomass (∼18 mg chl m⁻³) was characteristic of SEEDS. In contrast, in SEEDS II, the surface chlorophyll-a accumulation was lower, 0.8 to 2.48 mg m⁻³, with no prominent diatom bloom. Photosynthetic competence in terms of F _v/F _m for the total phytoplankton community in the surface waters increased after the iron enrichments and returned to the ambient level by day 20. These results suggest that the photosynthetic physiology of the phytoplankton assemblage was improved by the iron enrichments and returned to an iron-stressed condition during the declining phase of the bloom. Pico-phytoplankton (<2 μm) became dominant in the chlorophyll-a size distribution after the bloom. We observed a nitrate drawdown of 3.8 μM in the patch (day 21), but there was no difference in silicic acid concentration between inside and outside the patch. Mesozooplankton (copepod) biomass was three to five times higher during the bloom-development phase in SEEDS II than in SEEDS. The copepod biomass increased exponentially. The grazing rate estimation indicates that the copepod grazing prevented the formation of an extensive diatom bloom, which was observed in SEEDS, and led to the change to a pico-phytoplankton dominated community towards the end of the experiment.