养殖黄颡鱼(Pelteobagrus fulvidraco) 鮰爱德华氏菌(Edwardsiella ictaluri)的分离鉴定与生物学特性研究

从四川眉山与新津两地患红头病的养殖黄颡鱼体内分离到2株优势菌(CHNYC001与CHNYC002),以腹腔注射与浸泡的方式进行人工感染试验,证实其为养殖黄颡鱼红头病的病原菌。根据分离菌株的形态、生理生化特性,结合16SrDNA序列测定(GenBank登录号分别为FJ766524、FJ766525)与系统发育分析,将其鉴定为鮰爱德华氏菌(Edwardsie lla ictaluri)。分离菌株最适生长温度为20—30℃,最适生长pH为7.0,在含盐量2%以上的培养基上不生长,对氟苯尼考、强力霉素、洛美沙星等敏感,对乙酰螺旋霉素、磺胺甲基异噁唑和麦迪霉素等不敏感。临床病理特征分析发现,养殖黄颡鱼感染爱德华氏菌临床上分为急性与慢性两种类型,急性型主要表现为败血症的病变特征,慢性型表现为头顶出血,发红与溃疡的病变特征。     

池塘养殖脊尾白虾(Exopalaemon carinicauda)感染血卵涡鞭虫的研究

通过现场取样、显微镜检、细菌培养、组织病理、电镜观察以及PCR检测等研究手段,对2008年9月浙江省舟山市普陀区某梭子蟹养殖塘混养的脊尾白虾"白浊病"进行了系统的病原学研究。结果表明,病虾体色发白,反应迟钝,血淋巴液呈浊白色,严重患病白虾血淋巴中的血细胞几乎没有或者极少,代之以大量与血细胞形似的寄生虫;组织病理切片及电镜观察发现,病虾心脏、肝胰腺、肌肉、鳃部等组织寄生有大量单细胞寄生虫,该寄生虫虫体形态特征、显微结构及宿主临床症状与本实验室之前研究报道的感染三疣梭子蟹和锯缘青蟹的血卵涡鞭虫类似;采用已建立的针对血卵涡鞭虫ITS1基因的引物对"白浊病"病虾的血淋巴样品进行PCR检测,可扩增出585bp左右的特异条带。综合以上研究结果,确定该寄生虫为血卵涡鞭虫(Hematodinium sp.)。有关脊尾白虾感染该寄生虫的报道在国内外尚属首次。     

一种水产动物病毒现场检测免疫芯片的制备与应用

采用原子力显微镜对氨基化玻片及6种不同方法修饰玻片进行表面特征分析,并比较了硝酸纤维素(NC)膜、PVDF膜以及以上不同修饰玻片对抗体的固定效率、固定效果,最终选择琼脂糖修饰玻片作为芯片载体。将纯化后的兔抗血清(捕获抗体)点样至琼脂糖修饰玻片上,制备免疫芯片,与待检样品(病毒感染的靶器官组织)匀浆液孵育形成复合物,该复合物被辣根过氧化物酶(HRP)标记的特异性单克隆抗体(单抗)识别,经底物显色,得到肉眼可见的检测结果。通过改变芯片制备及应用过程中的具体条件参数,对各条件进行了优化,并采用生物素-链亲和素(BAS)标记特异性单抗作为检测抗体以提高检测灵敏度。基于此夹心免疫分析原理制备的WSSV、LCDV现场检测免疫芯片,病毒的最低检出量分别为82.50ng/ml、0.88μg/ml,在一定的抗原浓度范围内,病毒浓度的对数值与信号强度呈线性关系;采用BAS放大检测信号后,病毒的最低检出量为12.38ng/ml、0.22μg/ml。该免疫芯片与酶联免疫吸附法(ELISA)及免疫荧光法(IFAT)对同种样品的检测结果高度一致。     

陕西黑河流域地下水水化学特征

2017年在秦岭黑河流域7个采样点进行地下水样品采集。通过综合分析、Gibbs图、Piper三角图、主要离子比值法、主成分分析法得出黑河地下水化学成分的特点、水化学类型及其成因。结果表明:阳离子的主要组成成分是Ca^2+,HCO3^-是阴离子的主要组成成分,黑河流域地下水化学类型为HCO3^--Ca^2+和SO4^2--HCO3^--Ca^2+型。Gibbs图分析得出该地下水化学离子组成受岩石风化作用控制;Piper三角图、主要离子比值法、主成分分析及相关分析表明,地下水化学组分主要受方解石、白云岩等碳酸盐岩矿物的风化溶解,同时伴有硫酸溶解碳酸盐岩,受硅酸盐岩的溶解控制作用较小。     

可可西里苟仁错湖泊沉积物元素地球化学特征及其环境意义

湖泊沉积物元素地球化学特征及其比值，有效地揭示了湖泊沉积物物源区的化学风化程度．已成为反映古气候波动的有效指标。根据可可西里苟仁错湖泊沉积物的元素地球化学特征，通过CIA，ICV等元素比值的结果，揭示该地区1400AD以来化学风化作用十分微弱，因此湖泊沉积物元素组成，对气候变化非常敏感。研究表明1400AD以来该地区气候经历多次冷暖波动，且呈干旱化的趋势，1420AD左右进入小冰期，其中1480～1520AD最为寒冷，1900AD后干旱化加剧。     

三峡库区地质背景对土壤元素分布的影响及地方病效应

利用多目标地球化学调查方法,通过野外实地调查、大面积系统采集土壤表层样品、测试分析、数理统计,探讨了三峡库区灰岩母岩区与泥岩母岩区两种不同母岩条件下土壤中52个元素的含量分布特征。结果表明:三峡库区灰岩母岩区土壤元素富集而泥岩母岩区土壤元素贫乏,As、Br、B、Sb、C、F、I、Li、Mo、CaO等元素在灰岩母岩区土壤中含量大于泥岩区2倍以上。泥岩母岩区土壤元素含量比灰岩母岩区高的元素仅有Ba、P、Sr、Na₂O、SiO₂。元素分布的差异与三峡库区的地氟病与克山病分布关系密切,地氟病高发区主要分布于灰岩母岩风化形成的高氟土壤区,克山病则主要发生于泥岩母岩风化形成的低硒土壤区。地质背景对地方性疾病具有明显的区域控制作用,研究成果为三峡库区农业及城乡的合理规划布局提供了参考依据。     

Engineering characteristics and uses of duricrusts in Australia

The uses and shortcomings of duricrusts (ferricrete, calcrete and silcrete) in engineering construction (as used for road‐base, aggregate, foundation materials and aquifers) are reviewed. Australian production of these materials represents about one‐third of all unprocessed road‐base and they are especially important as pavement courses for lightly trafficked, low‐cost rural and outback roads. However, duricrusts are regarded as marginal materials at best because of their typically poor grading, particle unsoundness, high fines plasticity, and absorption of water and bitumen. These materials are used because they are available locally in areas that are otherwise lacking in hard rock materials, such as deeply weathered and sedimentary rock terrains. The weathering profiles of which they form part are characterized by high permeability (despite being clay‐rich), variable cementation, low compressibility and a tendency to become weaker with depth. Although the more indurated layers are unrippable, they are also difficult to blast. Pedogenic (nodular) duricrusts were formerly sought for natural road‐base, because they occur widely and require only rudimentary processing. However, well‐cemented groundwater (vadose) duricrusts are now the preferred deposits, even though they require crushing and screening. Ferricrete is the most widely exploited of the duricrusts for engineering purposes, especially in northern and southwestern Australia, although calcrete is important in South Australia and in the Murray Basin. Silcrete is only a minor source of aggregate and road‐base, mainly in western Queensland.     

The freeze-thaw weathering regime at a section of the Niagara escarpment on the Bruce Peninsula,Southern Ontario,Canada

The bedrock freeze-thaw and moisture regimes at an actively eroding site on the Niagara Escarpment, Bruce Peninsula, southern Ontario, were monitored between December 1983 and April 1984, and the results compared with amounts of debris collected in an adjacent rock trap. Frost wedging in pre-existing rock fissures is the primary mechanism responsible for the observed rockfall events; hydration effects are negligible. Debris production was more closely related to the duration of the freezing leg of the freeze-thaw cycle than to intensity or to cycle frequency. Release also coincided with periods of high pore saturation (> 60 per cent) and the seepage of water from cracks and fissures. Fewer freeze-thaw cycles were recorded in the air than at 1 and 3.5 cm in the bedrock. The number of cycles that could be declared geomorphologically effective according to established temperature criteria was normally less than half the total number of freeze-thaw cycles recorded in both air and bedrock. Under the current temperature regime at the field site, few effective cycles are capable of penetrating more than 5 cm into the free face.     

大连新港的红粘土及其工程特性的形成

在大连新港油罐区的建设中,发现了一种具有高孔隙性、高含水量、高塑性、中低压缩性的特殊土。作者对这种土及其母岩的矿物学、岩石学、地球化学、物理化学和工程特性进行了系统研究,揭示了该区红粘土及其工程特性的形成机理,该区红粘土乃是蒙脱石化的粗玄岩,在中温带气候区红土花作用的产物。     

论宁夏金场子渗流热卤水-表生改造型金矿床的矿源

稳定同位素、岩石微量元素(金)和地质特征研究的结果,表明金场子金矿床的成矿物质来源系上地壳源,并且金、银、铅、铜等金属和硫、硒、砷、氯、氟、碳等来源于石炭纪和泥盆纪沉积岩。成矿流体是大气水和沉积地层水的混合溶液。金矿化与地热流体渗流热卤水成矿作用和表生风化成矿作用有关。