中国对虾新病害—肠结节病的观察

1991年7～8月浙江省宁波、舟山地区一些中国对虾Penaeruschinensis的养虾场中爆发了一种肠结节新病害,危害十分严重。本文报导该病发生与危害的情况以及症状、病原、组织病理观察的结果。     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

我国渔用药物的研究内容及发展战略

近几年我国渔用药物的应用越来越广泛，渔用药物的研制和生产规模取得较大发展。本文对渔用药物的定义，主要研究内容进行了描述，对目前应用范围较广的药物进行了简单分类，并对渔用药物的现状进行了分析，提出了在研制，生产，销售和使用等方面存在的一些主要问题，最后对我国渔用药物发展战略提出了几点建议。     

对虾暴发性流行病及其预防研究

１９９３～１９９４年我国沿海养殖对虾发生了特大的暴发性流行病，损失惨重。本文报道该病的特点、症状及危害，并在患病的中国对虾、日本对虾、长毛对虾的中肠、肝胰腺的上皮细胞核和细胞质及腹肌纤维间质中发现大量具有囊膜的杆状病毒。通过病毒引起宿主靶组织细胞的病理变化、无包涵体等特性分析，认为该病毒属于有囊膜杆状病毒科Ｃ亚群，不同于已知的对虾所有杆状病毒，为对虾的一类新致病病毒。文中还分析了主要致病原因，通过调查和试验提出８条有针对性的预防措施。     

海水养殖鱼类细菌性疾病研究概况

本文概述性地介绍了发生在海水养殖中的常见细菌性疾病包括弧菌现、链球菌症、卡诺氏菌症、细菌性肾病、巴斯德氏杀菌症、爱德华氏症、滑走细菌症和杀鲑气单胞菌症等的主要症状、流行情况及病原菌特征，通过遗传育种筛选有抗病力的养殖品种是人们正在努力的方向，目前的研究重点主要在鱼类抗病能力方差分析研究及抗病育种的方法研究，免疫接种是提高养殖鱼类对某一特定性病原菌的最有效的方法，目前已有三十多种菌苗上市，通过使用免疫刺激剂提高鱼类的非特异性免疫力已显示一定的应用前景，虽然抗菌素由于存在抗药菌株和鱼体残留，但它仍是目前对细菌性疾病疾病的常规疗法，而海洋藻类提取物对细菌的抑制作用已被人们所认识，它将会成为一种有潜力的抗菌药物。     

鳗鲡气单胞菌引起的烂尾病病原菌抑菌药物的研究

应用药敏纸片法研究了三株烂尾病病原菌（气单胞菌）对３１种药物及不同配合药物的敏感性，测定了４种药物及配合药物ＨＰ对三株菌的最低抑菌浓度（ＭＩＣ）及最低杀菌浓度（ＭＢＣ）。结果表明，配合药物ＨＰ具较强的抑菌能力。     

凡纳对虾(Litopenaeus vannamei)溞状幼体黏脏病的治疗

用不同药物治疗凡纳对虾状幼体黏脏病 ,结果表明 :用浓度为 3× 10 - 6 养虾丹、混合施用 0 .15× 10 - 6 次氯酸钠 + 1.5mg/L土霉素或 0 .0 1× 10 - 6 的亚甲基蓝 + 1.5mg/L土霉素可治疗凡纳对虾幼体黏脏病     

虾池的蟹类及其药物杀灭

对山东半岛南海岸3个地点虾池内的蟹类组成、分布和携带病毒的情况进行了调查,并用敌百虫和次氯酸钠对天津厚蟹(Helicana fridens)进行了杀灭试验。发现虾池内的蟹类组成相对简单,蟹洞分布 85％集中在水位线以上 100 cm至水位线下 30 cm区域内;敌百虫对天津厚蟹的 96 h半致死浓度 LTD_(50)(96 h)=1.25×10~(-6),96 h全部将天津厚蟹杀死的浓度为 2.75×10~(-6);次氯酸钠(以有效氯计)对天津厚蟹的 72 h半致死浓度为 LTD_(50)(72 h)=63.8×10~(-6),72 h内将天津厚蟹全部杀死的浓度为 111.1×10~(-6)。     

Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins

The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows： Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control （group） had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations （Ca^2＋, Mg^2＋, Na^＋, Mn^2＋, Al^3＋, Fe^3＋, K^＋） were measured by ICP-OES, and the contents of water soluble phosphorus （Pws） and silicon （Siws） were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO3. The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis （2-DE）. The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the