凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

天然矿泉水特殊组分的地球化学初步研究

本文以ＧＢ８５３７－８７《饮用天然矿泉水国家标准》为依据，从理论上阐述了天然矿泉水特殊组分（锂、氡、锶、铁、锌、溴、氟、碘、钼、偏硅酸、偏硼酸、硒、二氧化碳）的物理化学性质，分布、岍存形式及迁移规律，以便对我国矿泉水勘察和开发能有所裨益。     

Incorporating Māori values into land management decision tools

ABSTRACT

Environmental sustainability and the long-term wellbeing of Māori (the indigenous people of New Zealand) are interdependent and degradation of landscapes risks the progressive degradation of Māori wellbeing. The present study developed an analysis framework based on Ki Uta Ki Tai (holistic-mountains to the sea- management philosophy advocated by Ngāi Tahu) for exploring relationships between landcover and Māori values to enable predictions of cultural values through space and time. We used this framework to predict how two Māori values (Overall Health and Cultural Land Use) have been altered as a result of landcover change between 2001–2012 in three Canterbury catchments. The area of native vegetation declined while exotic pasture increased between 2001–2012, and there were corresponding declines in both cultural health scores. These results suggest that the change in landcover has reduced the ability of the landscape to support Māori values. This framework for assessing changes in Māori values with respect to changing environmental conditions may identify opportunities for Māori to better engage in land use management decisions.     

红罗非鱼引进繁殖育种及海水驯养研究

作者对引进的红罗非鱼进行了生物学特性、繁殖育种及海水驯养等方面的试验研究,结果得出:①亲鱼的怀卵量与体长呈正相关(关系式为:Y=124.78L—588.8),在北方地区每年产卵3—4次,产卵量为怀卵量的1/2;②水温25—30℃,11—18d受精卵孵出鱼苗;③红罗非鱼不仅可在淡水中生存,且在自然海水和半咸水中均能生存,是海水养殖的优良品种;④红罗非鱼同虾混养,可提高鱼虾成活率,增大养成规格。     

Preliminary optical classification of lakes and coastal waters in Estonia and south Finland

A preliminary optical classification of lakes in Estonia and south Finland which can also be used for small bays of the Baltic Sea is elaborated. The classification is based on the optical properties of water (diffuse attenuation coefficient, diffuse reflectance) and parameters that are routinely monitored in water bodies (Secchi depth, concentration of chlorophyll-a, total suspended matter and yellow substance). The data complex used for our classification covers different types of water ecosystems (ranging from oligotrophic to hypertrophic) and the variability of water constituent concentrations in the ice-free period in Estonia and south Finland. Using cluster analysis, we found 5 optical classes of waters: clear (C), moderate (M), turbid (T), very turbid (V) and brown (B). There is satisfactory correspondence between class of water, shape of diffuse attenuation coefficient and diffuse reflectance spectra and trophic state of the lakes.     

Experimental activities that intentionally perturb the marine environment: Implications for the marine environmental protection and marine scientific research provisions of the 1982 United Nations Convention on the Law of the Sea

The global community's ability to assess the environmental effects of marine scientific research (MSR) as required by the 1982 United Nations Convention on the Law of the Sea's (UNCLOS) marine environment provisions (MEP) is limited, as shown by experimental activities that intentionally manipulate the marine environment. Such work enhances knowledge of the ocean, but it may also have significant environmental effects. This growing use of the ocean as a laboratory has global scientific, environmental, legal and policy implications. Examined here is the relationship between the MSR and the MEP provisions of UNCLOS. International, science-driven guidelines are proposed.     

North Pacific Subtropical Mode Waters Observed in Long XBT Cross Sections along 32.5°N Line

The S/V Shoyo, of the Hydrographic Department, Japan Coast Guard, has conducted high-density expendable bathythermograph (XBT) measurements along the 32.5°N line in the North Pacific every year from 1990 to 1993 as a part of the Japanese-World Ocean Circulation Experiment (WOCE). These XBT data are analyzed here, focusing on year-to-year variations of the inventory and core layer temperature (CLT) of the North Pacific subtropical mode water (NPSTMW). Large year-to-year changes are found in the NPSTMW CLTs estimated in longitudes between 140°E and 160°E. CLT values were found of 17.4°C in 1990, 17.1°C in 1991, 17.3°C in 1992 and 17.6°C in 1993. Inspection of the wintertime westerlies over the formation area and sea surface temperature distribution revealed that this change in CLT can be qualitatively attributed to the strength of atmospheric cooling in the formation area in the previous winter. Although a large year-to-year variation of NPSTMW inventory was also found, it is hard to state any relationship between CLT and atmospheric forcing. There is a possibility that different observational seasons may affect the inventory. It has also been found that the thermocline depth in 1991 was shallower in the sea area east of 180° than in 1992 and 1993. Associated with this change, the North Pacific central mode water (NPCMW), characterized by thermostad with temperatures ranging from 14°C to 11°C, appears in the sea area east of 180° in the 1992 and 1993 cross sections. The 1993 cross section, which ranged from the Japanese coast to the west coast of North America, possessed another thermostad in the surface layer, with a temperature of about 17°C in the eastern part of the cross section, off California. This revised version was published online in August 2006 with corrections to the Cover Date.