利用稳定同位素(15N)示踪技术研究浮游藻类氮素吸收速率特征

浮游藻类对溶解态氮的吸收同化是湖泊氮生物循环和水体富营养化发生机制探讨的关键环节。本文通过~(15)N稳定同位素添加实验以铜绿微囊藻(Microcystis aeruginosa)、海链藻(Thalassiosira sp.)、卡德藻(Tetraselmis sp.)、剧毒卡尔藻(Karlodinium veneficum)以及盐水隐藻(Rhodomonas salina)为研究对象,从浮游藻类氮素吸收时间、营养盐基质以及藻种差异三个方面研究五种藻类对铵氮(NH_4~+-N)、硝氮(NO_3~--N)、尿素氮(Urea-N)三种形态氮的吸收特征。研究发现:(1)浮游藻类对三种形态氮的吸收均在1h时吸收速率最高,其氮素吸收过程为快速吸收。(2)浮游藻类优先吸收还原态氮,其中NH_4~+-N吸收速率最高,当培养周期为1d和4d时浮游藻类对NH_4~+-N吸收速率的均值分别为4.05和4.15μmol/(L·h);浮游藻类对Urea-N吸收相对偏好系数为25.18—713.42,表现出对小分子溶解态有机氮的特定偏好性。(3)不同藻种对氮素吸收具有不同特征,其中,剧毒卡尔藻对三种形态氮的吸收速率均为最高,而铜绿微囊藻的吸收速率均为最低;不同藻种不同培养时间氮素吸收速率差异与浮游藻类生长周期等特性有关。不同浮游藻类对不同形态氮素表现出吸收特异性,对水体氮负荷和浮游藻类水华优势种形成将产生重要影响。     

透明胞外聚合颗粒物碳输运新途径

目前大家普遍认为,透明胞外聚合颗粒物(Transparent Exopolymer Particles,TEP)因其独特的凝聚效应导致碳通量向海底输出。但是,近几年的研究表明TEP不仅影响了碳沉降途径,其本身能够悬浮甚至向海水表层迁移,导致其在海洋微表层(Surface Micro-layer, SML)积累,最终显著影响海洋表层碳通量。TEP和其他颗粒物聚集形成凝聚物后,其运动趋势则由凝聚物中TEP的含量占比,即最终颗粒物密度所决定。一个新的值得注意的现象是,密度低的TEP通过与其他微粒聚合形成表面活性物质(Surface-active Substances, SAS),会在海洋–大气界面形成薄膜,显著影响海–气CO₂交换通量,甚至对全球气候变化造成影响。     

Immunotoxicity effect of benzo[α]pyrene on scallop Chlamys farreri

The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL⁻¹) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL⁻¹ B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL⁻¹ B[α]P treatment group and antibacterial activity in 0.1 μgL⁻¹ and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL⁻¹ B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms.     

温度、氮浓度和氮磷比对长心卡帕藻(Kappaphycus alvarezii)吸收氮速率的影响

分别在室内培养箱、海滨室外跑道池和不同自然海区,通过一次性和半连续添加营养、以及检测海区水质和藻体生长的方法,研究了不同氮浓度、温度和氮磷比条件下,长心卡帕藻氮吸收速率的变化和氮吸收速率随时间变化,以及栽培该藻的环境生态贡献。小型实验、中试放大和海区规模栽培结果表明:(1)在10—50μmol/L范围内,该藻吸收氮速率随氮浓度增加而增大;(2)当氮浓度一定时,氮磷比在1—50范围内对该藻吸收氮速率没有产生显著影响(P>0.05);(3)温度对该藻吸收氮速率有显著影响(P<0.05),其中温度在28℃时氮的吸收速率最高;(4)尽管一次性添加营养实验中长心卡帕藻吸收氮速率随时间变化表现出先快后慢的趋势,但是进一步的半连续添加营养实验证实,导致吸收速率下降系底物氮浓度限制,而不是藻本身吸收能力下降,结果还显示卡帕藻具有连续吸收同化无机氮能力;在自然光温度变化和不受底物浓度限制条件下,该藻藻体去除无机氮效率最大维持在0.3μmol/(gFW·h);(5)海南陵水黎安海湾水质数据显示,栽培该藻去除海水富营养化和净化水质作用显著,其去除海水富营养化的年贡献为33t氮素。     

中肋骨条藻和新月菱形藻对营养盐的吸收速率及环境因素影响的研究

于１９９５年３月－－１９９６年１月,通过实验室模拟培养,研究了中肋骨条菏和新月菱形藻对磷酸盐和硝酸盐的吸收速率,以及温度、光照等环境因素对新月菱形藻吸收磷酸盐的影响。结果表明,用Ｍｉｃｈａｅｌｉｓ－Ｍｅｎｔｏｎ方程处理,求得中肋骨条藻和新月菱形藻吸收磷酸盐的半饱和常数分别为０．６１μｍｏｌ／Ｌ和０．４８μｍｏｌ／Ｌ,吸收硝酸半饱和常数分别为７．８μｍｏｌ／Ｌ和６．０μｍｏｌ／Ｌ,新月菱形藻对磷酸盐     

营养盐因子对龙须菜和菊花江蓠氮磷吸收速率的影响

本文在实验室条件下,研究了营养盐因子对龙须菜和菊花江蓠N、P吸收速率的 影响,分别对不同氮和磷的浓度、氮磷比及不同化合态氮对这两种藻类氮、磷吸收速 率的影响进行了实验与分析．结果表明:在适宜范围内,两种海藻对氮、磷的吸收速率 随介质中氮、磷浓度的增加而增加;不同氮磷对两种海藻N吸收速率的影响不存在 显著差异,但对磷吸收速率的影响非常显著,最高对磷吸收速率在介质的氮磷比为 10:1时;海藻对NH4-N的吸收速率随着NO3-N／NH4-N比的降低而增加,对PO43-P的 吸收速率与对NH4-N的吸收相似,而对NO3-N的吸收速率与对NH4-N的吸收相反．     

2008年夏季加拿大海盆净二氧化碳吸收量评估

The third Chinese National Arctic Research Expedition(CHINARE) was conducted in the summer of 2008.During the survey,the surface seawater partial pressure of CO_2(pCO_2) was measured,and sea water samples were collected for CO_2 measurement in the Canada Basin.The distribution of pCO_2 in the Canada Basin was determined,the influencing factors were addressed,and the air-sea CO_2 flux in the Canada Basin was evaluated.The Canada Basin was divided into three regions:the ice-free zone(south of 77°N),the partially ice-covered zone(77°–80°N),and the heavily ice-covered zone(north of 80°N).In the ice-free zone,pCO_2 was high(320 to 368μatm,1 μatm=0.101 325 Pa),primarily due to rapid equilibration with atmospheric CO_2 over a short time.In the partially ice-covered zone,the surface pCO_2 was relatively low(250 to 270 μatm) due to ice-edge blooms and icemelt water dilution.In the heavily ice-covered zone,the seawater pCO_2 varied between 270 and 300 μatm due to biological CO_2 removal,the transportation of low pCO_2 water northward,and heavy ice cover.The surface seawater pCO_2 during the survey was undersaturated with respect to the atmosphere in the Canada Basin,and it was a net sink for atmospheric CO_2.The summertime net CO_2 uptake of the ice-free zone,the partially ice-covered zone and the heavily ice-covered zone was(4.14±1.08),(1.79±0.19),and(0.57±0.03) Tg/a(calculated by carbon,1Tg=10~(12) g),respectively.Overall,the net CO_2 sink of the Canada Basin in the summer of 2008 was(6.5±1.3) Tg/a,which accounted for 4%–10% of the Arctic Ocean CO_2 sink.     

铜藻(Sargassum horneri)在营养限制胁迫后对NH4-N的超补偿吸收研究

以经济马尾藻铜藻(Sargassum horneri)为研究材料,研究了其在营养限制胁迫后对NH_4-N的超补偿吸收情况。实验分营养限制和恢复营养两个阶段进行,每个阶段均设置饥饿处理组、饱和处理组和正常对照组。铜藻在低营养限制胁迫(饥饿)下培养10天后,恢复营养盐培养3天,分别采用次溴酸盐氧化法和锌镉还原法测定培养液中的NH_4-N和NO_3-N的浓度。本文研究发现,饥饿处理组中铜藻吸收氨氮的速率远高于正常对照组和饱和处理组,在恢复培养第一天时,饥饿处理组对NH_4-N的吸收速率最高为14.94μmol/(g·h),与正常对照组及饱和处理组间差异显著。在恢复培养的后两天,三个处理组中铜藻对NH_4-N的吸收速率差异慢慢变小,直至最后几乎相同。三组对NO_3-N没有表现出较高的吸收,最高仅为6.15μmol/(g·h),结果表明:氮源包括NH_4-N和NO_3-N时,铜藻优先选择吸收NH_4-N。实验后称重测定生长速率:对照组、饥饿处理组和饱和组生长率(SGR)分别为8.48%、8.86%、8.01%,ANOVA方差分析表明,三者存在显著差异(P=0.0320.05),从而证实了铜藻也存在超补偿生长的现象。     

Manganese in runoff from upland catchments: temporal patterns and controls on mobilization / Teneur en manganèse de l'écoulement en bassins versants d'altitude: variations temporelles et contrôles de la mobilisation

Abstract

Knowledge of the hydrochemical dynamics of the trace metal manganese (Mn) in upland catchments is required for water quality management. Stream water Mn and other solutes and flow were monitored in two upland catchments in northern England with different soils: one dominated by peat (HS7), the other by mineral soils (HS4). Maximum Mn concentrations occurred at different times in the two catchments: in summer baseflow at HS4 and during late summer storm events at HS7. A two-component chemical mixing model was used to identify the hydrological processes controlling Mn concentrations in stream water. This approach was more successful for HS4 than HS7, probably because of different processes of Mn release in the two catchments and also difficulties in selecting conservative solutes. Factor analysis of the stream water chemistry data set for each catchment was more useful in identifying the controls on Mn release into runoff. The factors indicate that the main source of Mn at HS4 is the hydrological pathway supplying summer baseflow, whereas at HS7 Mn is released during the rewetting of dried peat soils. Manganese concentrations in stream water in upland catchments appear to depend on soil type and antecedent moisture conditions. This has implications for the design of sampling strategies in upland catchments and also for managing the quality of water supplies from such areas.     

Estimation of source water to cedar elm in a central Texas riparian ecosystem

A riparian ecosystem downstream of a small dam in central Texas was instrumented for sap flow, soil moisture content, and stream level from 2001. Stable isotopes in water (D and ¹⁸O) were analysed from rainfall, stream, lake, and cored sapwood cellulose from cedar elm (Ulmus crassifolia). The isotope signature of water source to cedar elm was identified by back calculation starting with the water isotopes in cellulose, and accounting for leaf‐water evaporation and biological fractionation during cellulose synthesis. The estimated mean isotope of the source water to cedar elm was enriched above rainfall in similarity to stream water during 2002. Flow paths that may have contributed to estimated variability from regional base flow and recharge water were identified using the variably saturated HYDRUS‐2D model. Copyright © 2004 John Wiley & Sons, Ltd.