Induction of heat shock protein (hsp)60 in Isochrysis galbana exposed to sublethal preparations of dispersant and Prudhoe Bay crude oil

Adaptation to sublethal exposure to crude oil by phytoplankton is poorly understood. Use of chemical dispersants for oil spill remediation increases petroleum hydrocarbon concentrations in water, while exposing marine organisms to potentially toxic concentrations of dispersant. Heat shock proteins (hsps) have been found to serve as an adaptive and protective mechanism against environmental stresses. The objective of this project was to examine the induction of hsps in Isochrysis galbana, a golden-brown algae, following exposure to the water-accommodated fraction (WAF) of Prudhoe Bay crude oil (PBCO) and PBCO chemically dispersed with Corexit 9527® (dispersed oil: DO). Initial experiments using ³⁵S-labeled amino acids and 2-dimensional electrophoresis with subsequent western blotting identified and confirmed hsp60, a member of the chaperonin family of stress proteins, as being efficiently induced by heat shock in this species. One-dimensional SDS PAGE and western blotting, with hsp60 antibodies and chemiluminesence detection, were used to quantitate hsp60 following exposure to a range of environmental temperatures and concentrations of WAF and DO preparations. I. galbana cultured in 22 parts per thousand (‰) salinity showed a statistically significant increase (p<0.05) in hsp60 after exposure to 25, 30, and 35°C for 1 h compared to controls (20°C), while 34‰ cultures showed no significant increase in hsp60 with increasing temperature. Cultures (22 and 34‰) exposed to varying doses of WAF and DO exhibited statistically significant (p<0.05) increases in levels of hsp60 in all cases. A dose-related response was observed in all exposures (r²≥0.90) with the exception of 34‰ WAF. In independent exposures, a dose-related hsp60 response to naphthalene, a relatively abundant water-soluble aromatic hydrocarbon, was observed in both 22 and 34‰ cultures. Results of this study are consistent with previous studies in other species documenting increases in hsp60 levels with exposure to xenobiotics. Further studies are investigating the protective function of hsp60 against the toxic effects of exposure to WAF and DO preparations.     

Method discussion for quick response grey prediction of stronger aftershocks of an earthquake sequence

In this paper, we take occurrence process of early strong aftershocks of a main-after shock type′s earthquake sequence as a complex grey system, and introduce predicting method for its stronger aftershocks by grey predicting theory. Through inspection prediction for 1998 Zhangbei MS=6.2 earthquake sequence, it shows that the grey predicting method maybe has active significance for the investigation of quick response prediction problems of stronger aftershocks of an earthquake sequence.     

A study on seismic source process in short- term and imminent stage before destructive mining shock

ｎｔｒｏｄｕｃｔｉｏｎＳｔｕｄｙｏｎｓｅｉｓｍｉｃｓｏｕｒｃｅｐｒｏｃｅｓｉｎｓｈｏｒｔｔｅｒｍａｎｄｉｍｍｉｎｅｎｔｓｔａｇｅｂｅｆｏｒｅｅａｒｔｈｑｕａｋｅｉｓａｃｏｎｃｅｒｎｅｄｐｒｏｂｌｅｍｉｎｒｅｃｅｎｔｓｅｉｓｍｏｌｏｇｉｃａｌｆｉｅｌ...     

条石鲷(Oplegnathus fasciatus)雌核发育的人工诱导与鉴定

条石鲷(Oplegnathus fasciatus)是我国重要的经济鱼类,具有复性染色体性别决定型(X₁X₁X₂X₂/X₁X₂Y)。利用紫外线(UV)灭活的同源精子通过冷休克方法诱导了条石鲷的雌核发育,筛选精子紫外灭活剂量并优化冷休克相关参数。结果表明:条石鲷精子最适的灭活剂量为80 mJ/cm2雌核发育的最适条件为授精后2 min在3～4°C水温下处理10 min。条石鲷雌核发育子代经形态学和流式细胞仪鉴定为二倍体,进一步利用微卫星分析表明雌核发育后代的等位基因完全来自于母本没有父本基因参与。对子代的性比和生长进行了比较,结果表明条石鲷雌核发育后代均为雌性,15月龄后雌核发育后代比普通条石鲷具有更快的生长速度。研究结果为条石鲷单性苗种的培育及其性别决定机制的深入研究提供基础。     

Reduction of pounding effects in elevated bridges during earthquakes

Pounding of adjacent superstructure segments in elevated bridges during severe earthquakes can result in significant structural damage. The aim of this paper is to analyse several methods of reduction of the negative effects of collisions induced by the seismic wave propagation effect. The analysis is conducted on a detailed three‐dimensional structural component model of an isolated highway bridge. The results show that the influence of pounding on the structural response is significant in the longitudinal direction of the bridge and significantly depends on the gap size between superstructure segments. The smallest response can be obtained for very small gap sizes and for gap sizes large enough to prevent pounding. Further analysis indicates that the bridge behaviour can be effectively improved by placing hard rubber bumpers between segments and by stiff linking the segments one with another. The experimental results show that, for the practical application of such connectors, shock transmission units can be used. Copyright © 2000 John Wiley & Sons, Ltd.     

CHARACTERISTICS OF TECTONIC STRESS FIELD OF THE XIAOWAN RESERVOIR BEFORE AND AFTER THE IMPOUNDMENT

Using the seismic waveform data of Xiaowan seismic network and Yunnan seismic network, we determined the focal mechanisms of 36 earthquakes(M_L ≥ 3.0)from Jun. 2005 to Dec. 2008 and 51 earthquakes(M_L ≥ 2.5)from Jan. 2009 to Dec. 2015 by generalized polarity and amplitude technique. We inverted tectonic stress field of the Xiaowan reservoir before impounding, using the focal mechanisms of 36 earthquakes(M_L ≥ 3.0)from Jun. 2005 to Dec. 2008 and CAP solutions of 58 earthquakes(M_L ≥ 4.0)collected and the solutions in the Global Centroid Moment Tensor(GCMT)catalog; We inverted local stress field of the reservoir-triggered earthquake clustering area, using 51 earthquakes(M_L ≥ 2.5)from Jan. 2009 to Dec. 2015. Focal mechanisms statistics show that, the Weixi-Qiaohou Fault is the seismic fault. Focal mechanisms were strike-slip type in initial stage, but normal fault type in later stage. Focal depths statistics of 51 earthquakes(M_L ≥ 2.5)show that, the average value of focal depths in period Ⅰ, period Ⅱ and period Ⅲ are 8.2km, 7.3km and 7.8km respectively and the standard deviations are 4.3km, 3.5km and 6.0km respectively. The average value of focal depths is basically stable in different period, only the standard deviation is slightly different. Therefore, there is not positive connection between focal depth and deviation of focal mechanisms. What's more, there are 2 earthquakes(number 46 and number 47 in Fig.5 and Table 3)with almost the same magnitude, epicenter and focal depth, but they have different faulting types as normal and strike-slip. The focal mechanism of event No.46 is strike:302°, dip:40° and rake:-97° for plane Ⅰ, however, the focal mechanism of event No.47 is strike:292°, dip:82° and rake:140° for plane Ⅰ. Likewise, earthquake of number 3 and number 18 have similar characteristic. Therefore, the obvious focal mechanism difference of similar earthquake pair indicates the complexity of Weixi-Qiaohou Fault. Considering the quiet-active character of reservoir-triggered earthquakes, we discussed the change of local stress field in different period. The σ₁ of tectonic stress field was in the near-south direction, with a dip angle of 14° before the impoundment, however, the direction of σ₁ of local stress field changed continuously, with the dip angle getting larger after the impoundment. The direction of σ₁ of local stress field of reservoir-triggered earthquake clustering area is close to the strike of Weixi-Qiaohou Fault, and reservoir impoundment increased the shear stress in the fault, so the weakening of fault was beneficial to trigger earthquakes. Comprehensive analysis suggests that fluid permeation and pore pressure diffusion caused by the water impounding, and the weakening of fault caused by local stress field are the key factors to trigger earthquake in the Xiaowan reservoir.     

基于能量方程的建筑结构半主动控制

基于地震作用下结构的能量响应方程,本文提出根据结构吸收能量、相对动能和变形能的变化(对时间的一次导数)进行结构的“开-关”半主动减震的控制律;在物理坐标系中对这3种控制律的动力特征和控制效果,以及作动器参数的选取进行了分析。本文针对建筑结构进行了数值仿真。计算结果表明,本文提出的控制律可以有效地减小了结构的位移响应,对随机不确定的地震波均具有良好的减震效果,适应性强,而且控制器参数合理。其中根据结构变形能推导的控制律的半主动控制效果优于其它2种控制律。     

陨石中林伍德石的形成条件对寻找地球产状林伍德石的启示

橄榄石高压多形林伍德石被认为是地幔过渡带的主要矿物。天然产状林伍德石主要在发生强烈冲击变质的球粒陨石冲击脉体中出现。目前还没有在地球岩石中发现林伍德石的报告。陨石冲击脉体的温度压力历史和矿物组合特征研究表明,林伍德石形成后,高压下淬火是使林伍德石不发生退变作用的重要条件。陨石中有利于林伍德石保存的淬火时间仅为数秒到十多秒。在地球上任何地质事件中,均难以实现在如此短的时问内使位于地幔过渡带的林伍德石被带往地球表层。寻找地球产状的林伍德石,关键是要在岩石和矿物中存在有利于林伍德石保存的条件,特别是当这些岩石和矿物仍处于高温的环境时。     

Relaxation of protostellar accretion shocks using the smoothed particle hydrodynamics

It is believed that protostellar accretion disks are formed from nearly ballistic infall of molecular matter in rotating core collapse. Collisions of this infalling matter leads to formation of strong supersonic shocks, which if they cool rapidly, result in accumulation of that material in a thin structure in the equatorial plane. Here, we investigate the relaxation time of the protostellar accretion post‐shock gas using the smoothed particle hydrodynamics (SPH). For this purpose, a one‐dimensional head‐on collision of two molecular sheets is considered, and the time evolution of the temperature and density of the post‐shock region simulated. The results show that in strong supersonic shocks, the temperature of the post‐shock gas quickly increases proportional to square of the Mach number, and then gradually decreases according to the cooling processes. Using a suitable cooling function shows that in appropriate time‐scale, the center of the collision, which is at the equatorial plane of the core, is converted to a thin dense molecular disk, together with atomic and ionized gases around it. This structure for accretion disks may justify the suitable conditions for grain growth and formation of proto‐planetary entities (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

地球弓激波的旋转非对称性

通过对太阳风-磁层-电离层系统的全球MHD模拟,研究地球弓激波相对日地连线的旋转非对称性．模拟限于太阳风速度沿日地连线、地球磁偶极矩和行星际磁场（IMF）与日地连线垂直的简单情况．模拟结果表明,即便对于IMF强度为零的情况,弓激波相对日地连线也不具备旋转对称性质：终端面（晨昏子午面）及其向阳侧的弓激波截线的东西宽度大于南北宽度（约9%~11％）,终端面尾侧的弓激波截线东西宽度小于南北宽度（约8％）．在存在IMF的情况下,弓激波的位形同时受到磁层顶的形状和快磁声波速度各向异性的影响．磁层顶向外扩张并沿IMF方向拉伸,且其扩张和拉伸程度随IMF由北转南而增强．在磁鞘中,垂直于磁场方向的快磁声波速度高于平行方向．因此,磁层顶拉伸方向与快磁声波速度最大方向垂直,它们对弓激波位置的效应恰好相反;弓激波的最终形状取决于何种效应占据主导地位．对于终端面尾侧,快磁声波速度的各向异性起主导作用,弓激波截线沿IMF垂直方向的宽度大于平行方向．对于终端面及其向阳侧,弓激波截线的形状与IMF取向有关：在准北向或晨昏向IMF情况下,弓激波截线沿IMF垂直方向的宽度仍大于平行方向;在准南向IMF情况下,弓激波截线沿IMF垂直方向的宽度小于平行方向的．鉴于弓激波形状同IMF取向之间的密切关系,我们提议以IMF为基准方向,提取弓激波截线的平行半宽度R_b∥和垂直半宽度R_b⊥作为尺度参数．这些尺度参数和通常引入的弓激波截线的东西半宽度y_b和南北半宽度z_b相比,更为合理地表征了弓激波的几何性质．模拟结果表明,在终端面上,y_b/z_b和R_b∥/R_b⊥在IMF各向同性取向下的统计平均值均低于1,与观测得到的结论一致.