虎斑乌贼(Sepia pharaonis)FMRFamide G蛋白偶联受体基因的克隆与表达定位研究

以FMRFamide为典型代表的FMRFamide相关肽,是目前已知的最大的神经肽家族。FMRFamide广泛参与多种生理过程,包括摄食、心跳、渗透平衡、变态、防御和免疫等。采用同源克隆法与RACE技术获得了虎斑乌贼FMRFamide的G蛋白偶联受体基因cDNA全长序列(命名为SpFaGPCR,OL765295),SpFaGPCR cDNA全长1514bp,包括222bp的5''-非编码区(5''-UTR)、35 bp的3''-UTR以及1257bp的开放阅读框(ORF),共编码418个氨基酸。预测相对分子量(MW)为49.8kDa,等电点(pI)为9.76,具有7个跨膜结构域、糖基化位点7个、磷酸化位点36个。同源序列比对分析表明,SpFaGPCR与曼氏无针乌贼的FaGPCR氨基酸序列相似度最高达98%。系统发育分析显示SpFaGPCR与双壳纲的FaGPCR聚为姐妹支。荧光定量结果显示SpFaGPCR在视叶、视腺、脑、缠卵腺中表达量相对较高(P<0.05)。进一步利用原位杂交技术检测到虎斑乌贼视叶的髓质区、视网膜的感光细胞和缠卵腺的瓣叶外层具有明显的阳性杂交信号。实验结果为进一步探讨FMRFamide通过FaGPCR的介导参与虎斑乌贼生理代谢功能奠定了前期基础。     

谱模拟方法在高分辨率地震资料处理中的应用

为了克服地震子波的时变影响,避免复杂的时窗大小选取问题,使得谱模拟方法能更好地适应地震信号是平稳信号的假设条件,将改进S变换与谱模拟方法相结合,形成时频域谱模拟方法。在二维时频谱中进行谱模拟处理,该方法能够克服地震子波随地层深度变化的限制。理论模型及实际资料处理效果显示,时频域谱模拟方法能够有效提高地震分辨率,实现地震资料的高分辨处理。     

一种基于流形正则化的半监督指纹定位算法

针对传统指纹定位算法采集带标签训练数据成本高的问题,本文提出了一种基于流形正则化的半监督指纹定位算法。首先以流形假设为依据,利用批量输入的带标签数据与无标签数据之间的相似度构建图拉普拉斯算子;然后与极限学习机算法相结合,通过随机特征映射建立隐含层;最后在流形正则化框架下,求解隐含层和输出层之间的权值矩阵,从而建立位置估计模型。仿真结果表明,与INN、SVR、ELM 3种算法相比,该算法的训练和测试时间相对较短,且在带标签训练数据稀疏的前提下仍能保持较高的准确率与稳定性。     

考虑应变局部化的粗粒料剪切损伤力学机制

针对粗粒料的应变软化、剪胀等力学特性,通过考虑以剪切带为标志的应变局部化现象,建立了具有广泛适用性的剪切损伤力学模型。损伤模型采用了包体理论中的剪切带数学简化,基于应变等价原理、Weibull分布,推导了粗粒料的应力-应变关系方程。从剪胀作用的机制出发,提出可以描述剪胀弱化的轴向塑性应变和体积塑性应变的非线性函数关系。结合粗粒料三轴压缩试验中的伺服过程,提出了基于遗传算法的损伤模型参数确定方法。通过开展不同围压下的粗粒料三轴压缩试验,对剪切损伤力学模型进行验证,进一步分析了参数演化对粗粒料强度和变形特征的影响。研究结果表明,考虑应变局部化特征的剪切损伤力学模型可以高精度的模拟粗粒料的应变软化和剪胀等特征,有效揭示剪切带内部变形对试样整体宏观变形的影响机制,模型中剪切带参数和围压的关系与粗粒料细观机制一致,计算得到强度组成与颗粒破碎、重组特征较为吻合。     

面向室内WLAN定位的动态自适应模型

提出了一种面向无线局域网(wireless local area network,WLAN)位置指纹匹配定位的动态自适应模型,借助多个基站的实时数据为稳健室内定位提供更新的匹配数据库——radio map。基于基站与radio map采样点之间的空间关联性,将基站数据和radio map分别作为多层神经网络的输入和输出,动态更新radio map;利用多元数据异常探测技术检验基站数据捕获环境的时空变化;根据探测结果采用顾及室内布局的数值内插和再训练的方式更新模型,从而使其适应环境的时空变化。在室内动态环境中进行了多次验证试验。试验结果显示,在时变因素作用下,相比较传统方法,采用所提模型的定位方法的平均误差至少下降10%;在空间变化因素(以信标移动为例)作用下,其他方法平均定位误差大幅增加了至少165%,而采用所提模型方法的平均定位误差只增加了10%~20%,定位精度维持在3m左右(即原始精度)。结果证明采用了所提模型的定位系统能够自适应环境的时空变化而保持原有定位表现。不过,与传统位置指纹匹配定位方法相比,该模型带来了更多的计算负荷。     

Anatomy of a subduction complex: architecture of the Franciscan Complex,California, at multiple length and time scales

The Franciscan Complex of California records over 150 million years of continuous E-dipping subduction that terminated with conversion to a dextral transform plate boundary. The Franciscan comprises mélange and coherent units forming a stack of thrust nappes, with significant along-strike variability, and downward-decreasing metamorphic grade and accretion ages. The Franciscan records progressive subduction, accretion, metamorphism, and exhumation, spanning the extended period of subduction, rather than events superimposed on pre-existing stratigraphy. High-pressure (HP) metamorphic rocks lack a thermal overprint, indicating continuity of subduction from subduction initiation at ca. 165 Ma to termination at ca. 25 Ma. Accretionary periods may have alternated with episodes of subduction erosion that removed some previously accreted material, but the complex collectively reflects a net addition of material to the upper plate. Mélanges (serpentinite and siliciclastic matrix) with exotic blocks have sedimentary origins as submarine mass transport deposits, whereas mélanges formed by tectonism comprise disrupted ocean plate stratigraphy and lack exotic blocks. The former are interbedded with and grade into coherent siliciclastic units. Palaeomegathrust horizons, separating nappes accreted at different times, appear restricted to narrow zones of <100 m thickness. Exhumation of Franciscan units, both coherent and mélange, was accommodated by significant extension of the hanging wall and cross-sectional extrusion. The amount of total exhumation, as well as exhumation since subduction termination, needs to be considered when comparing Franciscan architecture to modern and ancient subduction complexes. Equal dextral separation of folded Franciscan nappes and late Cenozoic (post-subduction) units across strands of the (post-subduction) San Andreas fault system shows that the folding of nappes took place prior to subduction termination. Dextral separation of similar clastic sedimentary suites in the Franciscan and the coeval Great Valley Group forearc basin is approximately that of the San Andreas fault system, precluding major syn-subduction strike-slip displacement within the Franciscan.     

Spatial Autocorrelation and Localization of Urban Development

A nonlinear analysis of urban evolution is made by using of spatial autocorrelation theory. A first-order nonlinear autoregression model based on Clark’s negative exponential model is proposed to show urban population density. The new method and model are applied to Hangzhou City, China, as an example. The average distance of population activities, the auto-correlation coefficient of urban population density, and the auto-regressive function values all show trends of gradual increase from 1964 to 2000, but there always is a sharp first-order cutoff in the partial auto- correlations. These results indicate that urban development is a process of localization. The discovery of urban locality is significant to improve the cellular-automata-based urban simulation of modeling spatial complexity.     

数字化地震波形资料的时频分析方法及应用

信号的时频分布具有比时间尺度分布更高的描述和刻画非稳态地震波信号的能力,进一步提高信号的时频聚集性和尽可能减小交叉项干扰是时频分析发展的方向。基于高阶矩时频分布理论,本首次提出了将其应用于数字化地震波形资料分析和处理的技术途径和方法。通过对仿真信号的Choi-Willams各阶矩时频分布与Wigner各阶矩时频分布的对比和交叉项干扰的理论分析,认为Choi-willams高阶矩时频分布具有时频聚集性高和交叉干扰项小的特点,在提高处理数字化地震波特性参数精度和地震应急技术系统的智能化程度等方面具有潜在应用前景。     

光纤时间传输及相位补偿

介绍了国外几种利用光纤进行时间频率传递的方法和经验.对无补偿光纤时间频率传递方法、双向时间频率传递方法、光学机械温度补偿方法及电子共轭相位补偿方法作了较详细的描述.光纤时延主要随温度而变化,在200 km以内,时延的日变化为几纳秒,月变化为十几纳秒.在50 km内利用光纤传输100 MHz频率信号时,在不补偿情况下频率稳定度为: 3×10-14/s,1×10-15/d;光学补偿后的频率稳定度可达到1.5×10-14/s,1×10-17/d.电子共轭相位补偿后,温度变化20℃引起的相位变化降低了45倍.光纤传输对短期频率稳定度影响较小,对日及更长期的频率稳定度影响较大.