Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

唐山市滨海湿地动态演变特征及其机制分析

利用历史资料和Landsat TM卫星遥感图像,研究了唐山市滨海湿地自建国以来的演变特征,着重对1987~2004年陡河———滦河之间的滨海平原湿地类型进行了识别,绘制了不同时期的景观面解译图。利用Mapinfo制图软件计算出两个时期的面积(比例)转化矩阵,显示该区滨海湿地破碎化加重,其动态演变特征呈明显退化趋势,进而提出引起其退化的原因和发展趋势预测。     

The impact of the M2 internal tide on data-assimilative model estimates of the surface tide

We use a synthetic data experiment to assess the accuracy of ocean tides estimated from satellite altimetry data, with emphasis on the impact of the phase-locked internal tide, which has a surface expression of several centimeters near its sites of genesis. Previous tidal estimates have regarded this signal as a random measurement error; however, it is deterministic and not scale-separated from the barotropic (surface) tide around complex bathymetric features. The synthetic data experiments show that the internal tide has a negligible impact on the barotropic tidal fields inferred under these circumstances, and the barotropic dissipation (a quadratic functional of the tidal fields) is in good agreement with the energetics of the three-dimensional regional primitive equations model which is the source of the synthetic data.     

利用卫星测高技术监测厄尔尼诺和拉尼娜现象

利用美国宇航局和法国空间局联合公布的T／P数据（1993-2000年）和Jason-1数据（2002—2006年），由共线法计算了热带太平洋地区海平面高度的变化。根据T／P和Jason-1数据计算的海面月变化异常图，分别研究了和分析了1997-1998年的厄尔尼诺和拉尼娜现象、2002-2003年厄尔尼诺现象的变化发展过程。     

呼伦湖的近期扩张及其与全球气候变化的关系

通过近２０多年湖泊水量平衡分析发现，湖泊水位变化主要由湖盆内径流补给量的丰枯决定。进一步分析揭示，呼伦湖地区乃至整个东北地区，本世纪以来随气温升高，随水有增加的趋势。降水增加导致入湖径流量，湖水位上升，呼伦湖本世纪以来的扩张与内蒙古东部地区其他内陆湖泊的变化一致，但这在我国乃至整个亚洲内陆干旱或半干旱区是独一无二的，为此成为这一地区气候变化的指示器。     

Numerical study of enhanced energy dissipation near a seamount

Using a two-dimensional primitive equation model, we examine nonlinear responses of a semidiurnal tidal flow impinging on a seamount with a background Garrett-Munk-like (GM-like) internal wavefield. It is found that horizontally elongated pancake-like structures of high vertical wavenumber near-inertial current shear are created both in the near-field (the region over the slope of the seamount) and far-field (the region over the flat bottom of the ocean). An important distinction is that the high vertical wavenumber near-inertial current shear is amplified only at mid-latitudes in the far-field (owing to a parametric subharmonic instability (PSI)), whereas it is amplified both at mid-and high-latitudes (above the latitude where PSI can occur) in the near-field. In order to clarify the generating mechanism for the strong shear in the near-field, additional numerical experiments are carried out with the GM-like background internal waves removed. The experiments show that the strong shear is also created, indicating that it is not caused by the interaction between the background GM-like internal waves and the semidiurnal internal tides. One possible explanation is proposed for the amplification of high vertical wavenumber near-inertial current shear in the near-field where tide residual flow resulting from tide-topography interaction plays an important role in transferring energy from high-mode internal tides to near-inertial internal waves.     

利用地下水水位资料推求水文地质参数

依据模糊集合理论,提出了反求水文地质参数的一种新方法,即用模糊模式识别评价计算水位与观测水位的拟合程度,依模糊ISODATA聚类法的原理建立目标函数,以相应的聚类中心作为要求的水文地质参数。     

派比安台风对上海黄浦江潮位的影响及成因探讨

2000年派比安台风产生的历史第二高潮位对上海的防汛带来严重影响。本文通过比较历史上的风暴潮，从动力机制、天文高潮与增水极值相碰头，潮波共振和水利工程的影响等四个方面分析了这次风暴潮造成的高水位，及台风余振期边缘波的影响，并用SLOSH（Sea,Lake,Overland Surge from Hurricanes)模式模拟了这次风暴潮，为防台减灾的正确决策提供了理论依据。     

Anomalous change of the Antarctic sea ice and global sea level change

ＡｎｏｍａｌｏｕｓｃｈａｎｇｅｏｆｔｈｅＡｎｔａｒｃｔｉｃｓｅａｉｃｅａｎｄｇｌｏｂａｌｓｅａｌｅｖｅｌｃｈａｎｇｅ￥ＸｉｅＳｉｍｅｉ;ＺｏｕＢｉｎｇ;ＷａｎｇＹｉａｎｄＢａｏＣｈｅｎｇｌａｎ（１．ＮａｔｉｏｎａｌＭａｒｉｎｅＥｎｖｉｒｏｎｍｅｎｔＦ...     

应用遗传神经网格方法分析赤潮监测数据

简要介绍了遗传神经网络方法的原理 ,探讨了应用遗传神经网络方法研究辽东湾海域丹麦细柱藻 (Leptocylindrusdanicus)赤潮与其环境因子之间的关系 ,计算了各环境因子对丹麦细柱藻赤潮的贡献。结果表明 ,温度、盐度、DIN的变化对研究海域丹麦细柱藻种群密度的增长有比较重要的影响 ,DIN是营养限制因子。遗传神经网络是分析赤潮监测数据的有效方法 ,值得进一步探索