港口爆破施工的主要环境影响及评价方法

通过对常见的三种港口爆破施工方法——炸礁、爆破挤淤以及开山填海等的介绍,分析炸礁、爆破挤淤以及开山填海主要环境影响,并提出对三种施工方法环境影响的预测评价的方法。     

载人潜水器圆柱形耐压壳体的优化设计研究

采用分枝定界法和序列二次规划方法,对载人潜水器圆柱形耐压壳体的重量最小化进行了研究.设计变量是壳板的厚度、肋骨的型号、间距和数量,讨论了下潜深度、材料几何参数对重量以及其它特征量的影响.算例计算表明,下潜深度越大,屈服极限越高的材料重量减轻越明显.对于大深度而言,选用高屈服极限材料,可以使得材料能够充分利用.文中还对结构重量占排水量比例随深度变化的情况进行了研究.     

世界主要海洋强国综合实力测评研究

文章在回顾世界海洋强国发展历程和分析现阶段发展特点的基础上,构建国家层面的海洋综合实力评价指标体系,并结合层次分析法和加权积决策理论建立了综合实力测评模型。通过选取世界上8个有代表意义的海洋强国,结合其海洋综合实力的现状分析,利用测评模型对其进行测评排序,为我国"海洋强国"战略的科学规划提供参考。     

网格剖分积分法计算富钴结壳资源量

在矿区地质模型建立及边界条件限定的基础上,提出了基于网格剖分积分计算富钴结壳资源量的评估计算方法,推导了相应的计算公式,设计了相应的模块界面,并在"海底地形地貌自动成图系统"中实现了这种计算方法.这种资源量计算方法可应用于富钴结壳资源研究和评价计算.     

A method of determining dominant wave direction from multiparameter hydrological gauge measurements and its application

Multi-parameter hydrological gauge is an instrument developed by the South China Sea Institute of Oceanology,the Chinese Academy of Sciences to make simultaneous observation of current, wave, tide, water temperature and conductivity.By using the well-known "PUV method", the directional wave spectrum can be calculated and the dominant wave direction is then obtained.The comparison of the dominant wave directions derived from the measurements using both the multi-parameter hydrological gauge and the MARK Ⅱ "Wave-Rider" directional buoy shows that the dominant wave directions derived from the two kinds of measurements are in good agreement.     

Decomposition analysis on direct material input and dematerialization of mining cities in Northeast China

Material dematerialization is a basic approach to reduce the pressure on the resources and environment and to realize the sustainable development. The material flow analysis and decomposition method are used to calculate the direct material input (DMI) of 14 typical mining cities in Northeast China in 1995-2004 and to analyze the demateri-alization and its driving factors in the different types of mining cities oriented by coal, petroleum, metallurgy and multi-resources. The results are as follows: 1) from 1995 to 2006, the increase rates of the DMI and the material input intensity of mining cities declined following the order of multi-resources, metallurgy, coal, and petroleum cities, and the material utilizing efficiency did following the order of petroleum, coal, metallurgy, and multi-resources cities; 2) during the research period, all the kinds of mining cities were in the situation of weak sustainable development in most years; 3) the pressure on resources and environment in the multi-resources cities was the most serious; 4) the petro-leum cities showed the strong trend of sustainable development; and 5) in recent years, the driving function of eco-nomic development for material consuming has continuously strengthened and the controlling function of material utilizing efficiency for it has weakened. The key approaches to promote the development of circular economy of min-ing cities in Northeast China are put forward in the following aspects: 1) to strengthen the research and development of the technique of resources' cycling utilization, 2) to improve the utilizing efficiency of resources, and 3) to carry out the auditing system of resources utilization.     

基于部件可靠度和多阶段动态库存的备件管理决策

基于部件可靠度和多阶段动态库存理论,分析了备件需求与库存管理。提出了基于部件可靠度的动态需求模型;以部件的寿命服从指数分布为例,结合实际生产中备件的使用情况,通过确定备件储备的时间点,动态确定各周期备件的需求量。在确定备件动态需求量的基础上,采用多阶段动态库存理论确定了订货次数和每次订货数量,使库存量更符合实际和趋于合理。通过算例分析,给出了最小库存费用,表明基于部件可靠度和多阶段动态库存的备件管理具有一定的实际意义。     

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

Mapping the farming-pastoral ecotones in China

Conventional farming-pastoral ecotones methods of delineating were not quantitative and could not fully show their spatial distribution. The present paper attempts to develop quantitative methods for mapping farming-pastoral ecotones in China. Nine indicators, related to temperature, precipitation and altitude aspects, were selected to quantify ecological susceptibility of vegetation （crops and forage）. Methods of analytic hierarchy process （AHP） and expert score ranking combined with fuzzy set theory were applied to assign the weight for each indicator and to define the membership functions. The geographic information system （GIS） was used to manage the spatial database and conduct the spatial analysis. According to the spatial calculation of evaluation model integrated with GIS, the ecological susceptibility of vegetation （crops and forage） was mapped. Three different zones, pastoral area, farming-pastoral ecotones and farming area, were classified by spatial cluster analysis and the maximum likelihood classification for the numeric map of vegetation ecological susceptibility by GIS. This map was validated by the economic statistical result based on the ratio of the output value from animal husbandry in total output value of agriculture by the National Bureau of Statistics in China, indicating that the mapping of the farming-pastoral ecotones may be accepted.