Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

�����Բ���ʽԼ��ƽ��ģ�͵�һ������㷨

????Lemke???????в??????????????????????????????????в???????????С?????????????????ι滮?????????????ι滮??Kuhn??Tucker????????ι滮?????????LCP??????????Lemke?????????????????ò?????С????????????????????????????????á?     

������ģ������С���������ڶ�Դ���������ں��е�Ӧ��

????????С????????????????????ж???????????????????????????????????????????????????????????????????顣?????????????????С??????????????????????3.6??10????^-5??ms????^-2??????????????С???????????????????????????????????????????????????4.6??10????^-5??ms????^-2??????????????????????????????????????С?????????????????????????????п???????Ч?????     

威海市近海海域表层沉积物环境质量评价

对威海近海海域127站位表层沉积物中的重金属元素进行分析测试,采用单因子污染指数法、综合污染指数法、超标分类法和潜在生态危害法等多种评价方法对研究区环境质量进行评价。认为研究区海底表层沉积物的单因子污染物指数偏低,只有Cu和Cr两种重金属出现一类沉积物质量超标情况;海底表层沉积物环境质量在整体上处于清洁和尚清洁状态,个别站位处于允许状态;污染类型主要以Ⅰ类、Ⅱ类为主,含少量Ⅲ类,Ⅱ类又分为Ⅱ1Cr和Ⅱ2Cu两个污染亚类,Ⅲ类又分为Ⅲ1Cr-Cu和Ⅲ2Cu-Cr两个污染亚类;各种重金属的生态危害程度均低。     

论热动平衡判据有关的几个问题

对平衡态和线性准平衡态的稳定性及其与力学中相关概念的相似问题作了论述，并就蒸气中液滴的不稳定性进行了讨论。     

内蒙古鲁新井田水文地质条件研究

矿产资源开发导致了地下水失衡,地下水失衡又给矿产开发造成了极大的安全隐患,近年来,矿产资源开发与地下水环境保护之间的矛盾愈发凸显。通过对内蒙古鲁新井田典型的水文地质条件进行分析研究,分析采矿导致地下水失衡机理,深入剖析矿井开采充水条件及矿山开发对地下水环境的影响,合理提出了促进矿产开发与地下水保护相互协调的对策建议,为实现"采矿保水"协调统一提供了基础地质依据。为类似地区矿产开发过程中遵循自然规律,趋利避害,保障生产安全,保护地下水环境安全,实现资源绿色开发有较好的指导借鉴作用。     

烟台市地质灾害发育现状及影响因素分析

2016年山东省开展各市地质灾害排查工作,烟台市作为地质灾害较发育区,是该次排查重点工作区之一。该文以现状调查为基础,查明了烟台市共发育471处地质灾害隐患点,地质灾害规模以小型为主,主要类型为崩塌、滑坡、泥石流、采空塌陷和岩溶塌陷,其中崩塌隐患点占地质灾害总数的68%,数量最多,其次为泥石流和采空塌陷,各占总数的15%和14%,滑坡数量最少,仅占总数的1%,并介绍了各类地质灾害分布特征。在此基础上,对地质灾害的主要影响因素——地形地貌、岩土体类型、地质构造、降水和人类工程活动等进行了分析。最后提出了在进行工程建设活动的同时重视其引发的地质灾害问题,以减少和预防其带来的次生地质灾害。     

附有限制条件的间接平差秩亏时解法初探

在附有限制条件的间接平差的应用中,若所列观测方程中实际用到的未知参数的个数少于所选参数的总数u时,就会出现法方程系数阵秩亏的问题。为了解决此问题,在理论公式推导的基础上,给出了一种新的解决方法,并结合算例进行了验证。     

西安地热田医疗热矿水的产出状况分析

本文介绍了西安地热田概况及开采现状, 重点讨论了西安地热田医疗热矿水的产出状况     

A Sensitivity Study of Arctic Ice-Ocean Heat Exchange to the Three-Equation Boundary Condition Parametrization in CICE6

In this study, we perform a stand-alone sensitivity study using the Los Alamos Sea ice model version 6(CICE6) to investigate the model sensitivity to two Ice-Ocean(IO) boundary condition approaches. One is the two-equation approach that treats the freezing temperature as a function of the ocean mixed layer(ML) salinity, using two equations to parametrize the IO heat exchanges. Another approach uses the salinity of the IO interface to define the actual freezing temperature, so an equation describ...