广西红树林根际土壤放线菌的原位培养分离及其活性筛选

为了发掘广西红树林根际土壤的放线菌资源,本文利用原位培养装置,埋于根际土壤中俘获放线菌,30d后取回实验室,采用平板涂布法对4个地点的原位培养样品于15种培养基上进行分离纯化;对分离株基于16SrRNA基因序列进行系统发育分析;进一步进行抗菌活性和产酶活性检测。共分离得到113株放线菌。对其中33株放线菌进行测序,结果表明20株属于链霉菌属,11株属于拟诺卡氏菌属,1株属于伦兹氏菌属,1株与拟诺卡氏菌属相似性最高为90%,很可能属于放线菌一个新属。抗菌实验结果显示其中有7株、4株、18株、6株、10株、3株实验菌株分别对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、普通变形杆菌、乙型溶血性链球菌和肺炎克雷伯氏菌具有抑制作用;有55株、62株、24株、72株的实验菌株分别具有纤维素酶活性、淀粉酶活性、胶原蛋白酶活性、酯酶活性。原位培养可以丰富对广西红树林根际土壤的认识,分离到了新种甚至可能是新属的放线菌,分离得到的部分放线菌菌株具有较高生物活性,为后续工作提供了良好的实验材料。     

Alkaline Phosphatase Activity in the Red-Tide Dinoflagellate, Ptychodiscus brevis

Abstract. Alkaline phosphatase activity (APA) was found in a nearshore, natural population of the red-tide dinoflagellate Ptychodiscus brevis. Initial hydrolysis rates were enhanced by two orders of magnitude within 24 h upon addition of ammonium. Other nutrient (additions) or no additions also lead to a higher, although less pronounced increase in activity. Enhanced growth after nutrient additions combined with specific APA indicated that the bloom was both N and P limited. Since the natural population was a mixture of diatoms and the dinoflagellate, APA was also measured in unialgal cultures of P. brevis. APA was not reduced but enhanced by the addition of low concentrations of orthophosphate (0.5 and 1 μM). Higher orthophosphate concentrations (5 to 20 μM) reduced APA, although activity returned to control levels within 2 to 6h. Repression occurred within 30 sec after a 20 μM orthophosphate addition. Cells which had APA repressed by an orthophosphate addition regained their initial activity within 2 h after washing with phosphate-free media. Phosphorus-deficient cells of P. brevis possess an alkaline phosphatase system which is capable of continued activity at low in situ orthophosphate levels.     

大型海藻孔石莼化学组分和生物活性研究新进展

孔石莼是一种大型绿藻,在食品、药物等领域具有广泛应用。本文综述了近5年来孔石莼化学组分及其生物活性研究进展,并展望了其应用前景。     

珠江口盆地东沙隆起的沉降史及其动力机制

根据东沙隆起及其周围钻井资料的一维构造沉降对比分析,结合前人在基底、盖层及断裂等方面的研究,将东沙隆起地区的构造演化分为5个阶段:自垩纪挤压阶段、古新世-早渐新世伸展断陷阶段、早渐新世末期抬升剥蚀阶段、晚渐新世-中新世快速的裂后沉降阶段、晚中新世以来断块升降阶段.认为下地壳高速层是促使早渐新世末期抬升剥蚀的重要因素,而...     

Shallow-Water Gasohydrothermal Vents of Ushishir Volcano and the Ecosystem of Kraternaya Bight (The Kurile Islands)

Abstract. A marine ecosystem in the crater of the Ushishir Volcano (Kraternaya Bight, Yankich Island, the Kuriles) showing gasothcrmal activity was studied for hydrographical, hydrochemical, and biological characteristics. Maximal changes in chemical and biological characteristics were observed in the surface water layer of 0–5 m. This layer had higher water temperature, was saturated with volcanogcnic carbon dioxide (up to 2000 10^-6 torr), ovcrsaturatcd with oxygen (up to 200 %), and contained high concentrations of chlorophyll a. Hydrogen sulfide was found in the surface water layer and at a depth of 15 m in the area of maximal effect of volcanic effluents.
The planktonic community is characterized by high rates of production and destruction of organic matter only in the 0–5 m layer. Daily vertical migrations of the ciliatc Mesodinium rubrum were observed, which caused "red tides".
Bacterial, algobacterial, and diatom mats developed on the bottom of the bight in the zone of gasohydrothermal vents and in areas of volcanic water seeping. The rate of organic matter production in algobacterial mats reached 33.4g C-rrr²-d^-l, chcmolithotrophy predominating. Bottom settlements had high population density and great animal biomass, which reached 10 kg m^-2 in gasohydrothermal fields. They obtain sufficient energy from primary production of microphy-tobenthos, algobacterial, and bacterial mats. Bcnthic species dominant in the bight were not found off the Ushishir Islands.
Some species of macrobenthos inhabiting the Kraternaya Bight differ markedly in size and biomass from the same species found beyond the bight.     

Sources and hydrothermal alteration of organic matter in Quaternary sediments: A synthesis of studies from the Central Gulf of California

Deep sea drilling in the Central Gulf of California, a young and active spreading centre, shows that the high heat flow typical of these regions causes extensive alteration of sediment organic matter, especially near sills and above magma chambers where hydrothermal activity is concentrated. Even on the nearby passive margin, where there are no sills, heat flow is moderately high and hydrocarbon generation has begun in immature sequences. Migrating light hydrocarbons are detected especially where hydrothermal activity is concentrated. Thermogenic methane is more widespread, though not in the passive margin bordering the spreading centre. Despite the incidence of hydrocarbon generation and migration, the amounts of hydrocarbons involved are relatively small and apparently do not lead to commercially significant accumulations of petroleum.The organic matter in these sediments is mostly marine because the Gulf of California generally has low runoff from land and highly productive surface waters. Turbidites rich in terrigenous organic material are locally abundant in the mainly pelagic section in the Guaymas Basin. The highest concentrations of organic matter are found in laminated diatomites deposited on the Guaymas passive margin within the oxygen minimum zone.     

太平洋三海区热液烟囱物的氧同位素特征及形成温度

使用氧同位素方法 ,测试了西太平洋马里亚纳岛弧、马里亚纳海槽、冲绳海槽和东太平洋加拉帕戈斯裂谷的海底热液烟囱和硫化物全岩样品。结果表明 :马里亚纳岛弧上的埃斯梅拉尔达破火山口的热液硫化物的形成温度最高 (达 2 75℃ ) ,而成为高温产物的代表 ;冲绳海槽和加拉帕戈斯裂谷烟囱 ,表现为中高温类型 ,其氧同位素温度在 1 5 0℃左右 ;马里亚纳海槽以蛋白石为主要矿物的烟囱物 ,则显示出典型的低温热液类型 ,少量黄铁矿砂试样则表现出中温类型特征。但上述区域大都不同程度地存在有闪锌矿、黄铁矿、黄铜矿等中高温热液矿物 ,说明研究区除马里亚纳海槽明显有中低温热液活动类型外 ,其它区域皆具备中高温热液活动特征     

辽西凸起南段沙河街组双向物源沉积模式及油气勘探意义

古近系储层发育程度一直是制约辽西凸起南段勘探突破的关键因素。运用钻井地质、磷灰石裂变径迹分析、三维地震等基础资料,针对辽西凸起南段沙河街组储层发育问题,开展精细古热史-构造演化分析、古地貌半定量刻画,对目标区沙河街组储层进行预测,结果表明：辽西凸起南段主要经历了新生代早期构造抬升、古近纪沙河街组沉积期构造沉降、东营组沉积期构造反转和新近纪构造定型4个演化时期;断裂体系可划分为长期活动型、早断早衰型、晚断晚衰型3种断裂类型;辽西凹陷具有“盆满砂溢”的先天供源条件,构造演化的分段性特征又造成辽西凸起南段形成了特殊的“链状岛湾”沉积地貌,既可以为围区提供物源,形成近源沉积体,又可以形成较大的可容纳空间,汇聚来自于辽西凹陷满溢的砂体,在辽西凸起上形成了双向供源沉积模式。以上认识推动了旅大4-3油田的油气勘探评价,且对凸起围区的油气勘探具有较好的借鉴意义。     

吉林海沟金矿地质特征及应力场分析

海沟金矿是大型石英脉型金矿床,矿体严格受NE向断裂构造控制,成矿时代为晚侏罗世-早白垩世.矿石中金属矿物以黄铁矿、自然金、方铅矿和黄铜矿为主.28号主矿脉中,Au平均品位20.59×10-6.成矿物质来自地层和岩体.对构造应力场研究表明,矿区有3期构造应力场,分别是早期近S-N向挤压、中期NW向挤压和晚期NE向挤压.成矿期应力场最大主压应力方向为NE向.