对虾一种无包涵体杆状病毒病原的PCR检测

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.     

Upstream dams and downstream clams: growth rates of bivalve mollusks unveil impact of river management on estuarine ecosystems (Colorado River Delta, Mexico)

We studied how the extensive diversion of Colorado River water, induced by dams and agricultural activities of the last 70 years, affected the growth rates of two abundant bivalve mollusk species (Chione cortezi and Chione fluctifraga) in the northern Gulf of California. Shells alive on the delta today (‘Post-dam’ shells) grow 5.8–27.9% faster than shells alive prior to the construction of dams (‘Pre-dam’ shells). This increase in annual shell production is linked to the currently sharply reduced freshwater influx to the Colorado River estuary. Before the upstream river management, lower salinity retarded growth rates in these bivalves. Intra-annual growth rates were 50% lower during spring and early summer, when river flow was at its maximum. Growth rates in Chione today are largely controlled by temperature and nutrients; prior to the construction of dams and the diversion of the Colorado River flow, seasonal changes in salinity played an important role in regulating calcification rates.Our study employs sclerochronological (growth increment analysis) and geochemical techniques to assess the impact of reduced freshwater influx on bivalve growth rates in the Colorado River estuary. A combination of both techniques provides an excellent tool to evaluate the impact of river management in areas where no pre-impact studies were made.     

酶解条件对条斑紫菜单细胞成活率影响的比较研究

该文分别选用不同浓度的海螺酶 ;葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠 5种渗透剂 ;维生素 C和甘露醇 2种抗氧化剂 ;对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明 :用浓度为 10 %的海螺酶酶解条斑紫菜 ,成活率最高 ;选用 2 mol/ L葡萄糖作渗透剂 ,效果最好 ,成活率达85.2 % ;加入抗氧化剂对细胞成活率没有影响     

Estimation of Uplift Capacity of Rapidly Loaded Plate Anchors in Soft Clay

Plate anchors are extensively used in civil engineering constructions as they provide an economical alternative to gravity and other embedded anchors. The rate of loading is one of the important factors that affects the magnitude of soil resistance as well as soil suction force. This article outlines the effect of pullout rate on uplift behavior of plate anchors (70 mm diameter) buried in soft saturated clay by varying the pullout rate from 1.4 mm/min to 21.0 mm/min. The variation of breakout force and suction force with embedment depth and rate of pull are presented. A correlation between the rate of increase of undrained strength of clay and anchor capacity with rate of strain has been established. Finally an empirical equation has been proposed that includes the rate of pull in the estimation of breakout capacity of anchors.     

Discussion: A review on the behaviour of helical piles as a potential offshore foundation system

Abstract

Helical piles have emerged as an attractive foundation system for offshore applications with renewed interest from the offshore community. Significant research gap currently exists in transferring this technology offshore and this paper discusses how existing and emerging knowledge can be successfully used to bridge some of the gaps. We focus on the Coupled Eulerian Lagrangian (CEL) large deformation finite element (LDFE) modelling technique that is commercially available and can be used to model the three-dimensional installation process with consideration of strain rate and softening effects in soft offshore clays. A helical pile of L?=?7.5?m long is modelled with one or two large-diameter helices (D?=?2?m) attached to a central shaft of d?=?0.5?m in diameter.The net effect of strain rate and softening is to increase the installation torque. The measured torque is within the range of 200–400?kN.m for the offshore clay and the pile geometry studied. Additional helices increase the uplift force but to a lesser degree than that of the measured torque. Remoulding induced strength reduction is found to be within the range of 25–33% of the intact clay strength. Issues of extracting and reusing offshore helical pile foundations are discussed.     

Nutrient Limitation in Macroalgae (Ulva and Enteromorpha) from the Rías Baixas (NW Spain)

Abstract.  Photosynthesis and growth rates in samples of two green seaweeds ( Ulva and Enteromorpha ) were monitored in the laboratory. The macroalgae were collected every 15 days from four coastal embayments in Galicia (NW Spain). Ulva samples were found to be nutritionally limited, particularly in summer. The physiological activity of Enteromorpha did not appear to be as affected by nutrient scarcity, although it may have suffered from phosphorus limitation. The subsistence quotas, critical concentrations and storage capacity for N and P in Ulva were comparable to those obtained by other investigators in similar studies. The trace metals analysed apparently did not negatively affect the macroalgae at the measured concentrations; one explanation is that many of these metals function as essential micronutrients. The complex physiological interactions between metals and nutrients, however, may complicate interpretation of the results.     

Sediment Transport in Rivers and Coastal Waters

Following Bagnold's approach, a relationship between sediment transport and energy dissipation is developed. The major assumption made in the study is that the near bed velocity plays a dominant role in the process of sediment transport. A general relationship between energy dissipation and sediment transport is first proposed. Then the equations for total sediment transport are derived by introducing the appropriate expression of energy dissipation rate under different conditions, such as open channel flows, combination of wave and current, as well as longshore sediment transport. Within the flows investigated, the derived relationships are fairly consistent with the available data over a wide range of conditions.