梨树断陷断陷期小宽断裂带构造应力场数值模拟

在小宽断裂带构造解析基础之上,根据地质资料以及前人提供的岩石物理学参数建立了梨树断陷区的二维地质和数值模型。进行了构造应力场数值模拟,分析了梨树断陷小宽断裂带断陷期小宽断裂带断陷期在沙河子期(K1sh)、营城期(K1yc)、登楼库期(K1d)的构造应力场特征。模拟结果显示：(1)沙河子期在左旋走滑应力状态下,小宽断裂带处于近NE—SW方向的拉张应力状态,剪应力在沿着断裂带SW—NE走向逐渐减小; (2)营城期在右旋走滑的应力状态下,小宽断裂带处于近NW—SE向的拉张力状态,剪应力最大值位于断裂带的最中间部位;(3)登楼库期在左旋走滑的应力状态下,断裂带处于SWW—NEE向的张拉应力状态,剪应力最大值位于断裂带西南段。     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

Current-induced scour beneath initially elevated subsea pipelines

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.     

热锻炼对龙须菜高温胁迫响应的影响

夏季高温是限制龙须菜(Gracilariopsis lemaneiformis)生长和发育的一个主要问题。本研究以野生龙须菜作为材料,经过28℃的热锻炼预处理72 h,再分别置于32、36、40℃高温胁迫24 h,并测定了高温胁迫条件下龙须菜的丙二醛(MDA)、过氧化氢(H2O2)和可溶性蛋白质的含量,以及超氧化物歧化酶(SOD)活性和抗坏血酸(AsA)水平等生理指标,研究了龙须菜对高温胁迫的响应。结果表明:热锻炼能维持藻体较低的MDA含量,降低H2O2的产生速率,缓解藻体的膜脂过氧化伤害;同时热锻炼缓解了可溶性蛋白质的降解,增加了SOD酶的比活力,缓解了AsA的下降速率,说明热锻炼能缓解高温胁迫对藻体的伤害,有效地提高了龙须菜对高温胁迫响应的能力。     

Simulation of paleotectonic stress fields and quantitative prediction of multi-period fractures in shale reservoirs: A case study of the Niutitang Formation in the Lower Cambrian in the Cen'gong block,South China

Reservoirs where tectonic fractures significantly impact fluid flow are widespread. Industrial-level shale gas production has been established from the Lower Cambrian Niutitang Formation in the Cen'gong block, South China; the practice of exploration and development of shale gas in the Cen'gong block shows that the abundance of gas in different layers and wells is closely related to the degree of development of fractures. In this study, the data obtained from outcrop, cores, and logs were used to determine the developmental characteristics of such tectonic fractures. By doing an analysis of structural evolution, acoustic emission, burial history, logging evaluation, seismic inversion, and rock mechanics tests, 3-D heterogeneous geomechanical models were established by using a finite element method (FEM) stress analysis approach to simulate paleotectonic stress fields during the Late Hercynian—Early Indo-Chinese and Middle-Late Yanshanian periods. The effects of faulting, folding, and variations of mechanical parameters on the development of fractures could then be identified. A fracture density calculation model was established to determine the quantitative development of fractures in different stages and layers. Favorable areas for shale gas exploration were determined by examining the relationship between fracture density and gas content of three wells. The simulation results indicate the magnitude of minimum principal stress during the Late Hercynian — Early Indo-Chinese period within the Cen'gong block is −100 ∼ −110 MPa with a direction of SE-NW (140°–320°), and the magnitude of the maximum principal stress during the Middle-Late Yanshanian period within the Cen'gong block is 150–170 MPa with a direction of NNW-SSE (345°–165°). During the Late Hercynian — Early Indo-Chinese period, the mechanical parameters and faults play an important role in the development of fractures, and fractures at the downthrown side of the fault are more developed than those at the uplifted side; folding plays an important role in the development of fractures in the Middle-Late Yanshanian period, and faulting is a secondary control. This 3-D heterogeneous geomechanical modelling method and fracture density calculation modelling are not only significant for prediction of shale fractures in complex structural areas, but also have a practical significance for the prediction of other reservoir fractures.     

不同培养方式及换水频率对坛紫菜(Porphyra haitanensis)自由丝状体生长及藻胆蛋白含量的影响

本实验以坛紫菜自由丝状体为材料,研究不同培养方式(静置、摇床、通气)和换水频率(5d、7d、10d、20d)对丝状体生长及藻胆蛋白(PE、PC)含量变化的影响。研究结果表明:坛紫菜自由丝状体在摇床培养条件下生长较好,特定生长速率可达10.832 7%~10.891 5%;摇床培养下的丝状体PE含量相对静置培养的较高一些,通气培养的相对较低,最高为36.816 8mg/g;静置条件下培养的丝状体PC含量最高,摇床培养次之,通气培养最低,最低为1.082 4mg/g。相同培养方式下,换水频率对丝状体特定生长速率影响不大;除通气培养外,换水频率20d其PE、PC含量较高。方差分析结果表明:实验范围内的培养方式及换水频率对丝状体特定生长速率大小、PE和PC含量不存在交互作用。本文实验结果对坛紫菜自由丝状体产业化应用于食品加工、色素提取等产业提供了实验理论和依据。     

基于五种油源的中重度风化溢油的常规鉴定指标的应用效果评估

本文利用采集于我国三大油田的五种原油样品开展了长达210天的溢油风化模拟实验,并依据相对偏差和重复性限数学分析法,进行溢油风化过程分析和诊断比值应用效果评估。研究结果表明:经过210天的风化,溢油鉴定诊断比值发生明显改变;其中来源于萜烷、甾烷和多环芳烃的诊断比值变化率要远低于正构烷烃,可用于中长期风化溢油鉴定。此外,研究发现,在这些有效诊断比值中有4个变化率较小,相对偏差低于5%,保持了较好的稳定性,更适合于重度风化溢油鉴定。     

急性低氧胁迫对卵形鲳鲹选育群体血液生化指标的影响

本文研究了水环境急性低氧胁迫对卵形鲳鲹Trachinotus ovatus选育群体血液生化指标的影响。实验设计了急性低氧试验组和对照组,每组3个平行。结果显示,急性低氧胁迫后选育群体的血清离子含量与对照组相比都有不同程度的升高,其中钠、氯、钙浓度与对照组相比差异显著(p0.05),铁浓度极显著高于对照组(p0.01);血清蛋白、尿酸、肌酐、血脂、血糖等指标的差异较大,其中尿酸、肌酐极显著高于对照组(p0.01),总蛋白、总胆固醇显著低于对照组(p0.05);血清中肌酸激酶含量极显著高于对照组(p0.01),肌酸激酶同工酶显著高于对照组(p0.05),γ-谷氨酰转肽酶含量显著低于对照组(p0.05);其他指标均有不同程度的升高或降低,但差异不显著。研究表明,急性低氧胁迫造成卵形鲳鲹鳃、肾、心脏和肝脏损伤,免疫力下降。     

强壮前沟藻(Amphidinium carterae Hulbert)在多重逆境条件下的生长和生理响应

尽管单一逆境条件对浮游植物产生的影响已被广泛重视,然而对于多重逆境条件对藻细胞生长生理的研究仍有空白。本研究通过测定强壮前沟藻(Amphidinium carterae Hulbert)光密度(OD750)、叶绿素a含量(chl a)、光合作用效率(F_v/F_m)、碳氮比(C/N)等多个生理指标,分析其在9种环境下(常温光照、低温和低温黑暗三种物理环境,结合全营养、缺氮、缺磷三种营养状态)对多重逆境的生长和生理响应。研究结果表明,在多重逆境条件下,单一物理逆境因素(低温或黑暗)较氮限制或磷限制对藻细胞生长和生理的不利影响更为显著。营养限制并同低温环境双重作用对生物量和碳氮比产生显著性影响(P0.01)。此外,低温和黑暗条件耦合作用下,SYTOX Green染色强度处于较低水平,chl a稳定、F_v/F_m有所升高,强壮前沟藻在逆境环境下作为群体的衰亡得以缓解。