蓝隐藻藻蓝蛋白结构与功能稳定性研究

以蓝隐藻(Chroomonas placiodea)藻蓝蛋白 PC-645为材料,通过改变环境 pH 和尿素质量浓度,监测其变性和复性过程中特征荧光谱和吸收谱的动力学变化,以期了解隐藻藻蓝蛋白的色基和蛋白结构稳定性及其与功能的关系。结果表明, PC-645在很宽的 pH 范围和一定质量浓度的尿素中维持结构和功能的稳定,空间结构具有很强的柔性。pH诱导的 PC-645蛋白构象与功能变化可分为3个不同的区段。(1)稳定区(pH 3.5~7)：吸收和荧光光谱都比较稳定,显示蛋白质构象和功能在此区域都保持正常。(2)次稳定区(pH 7~10)：光吸收依然保持平稳,亚基内部的色基的状态和疏水微环境都没有改变,但荧光传递效率降低,可能是由亚基表面局部构象变化、解离(四级结构变化)或者色素基团间的空间距离变化引起。(3)不稳定区(pH<3.5和 pH>10),吸光度和荧光强度都呈快速下降,色基在近紫外和可见光区的吸收峰位变动,蛋白构象处于快速崩溃期。PC-645在酸性条件下的稳定性高于在碱性环境下,是与隐藻藻蓝蛋白所处的特殊环境及生理功能相适应的。     

Major Features of Topography,Geology, and Crustal Stability in the Taiwan Strait: A Scientific Approach to the Problem of the Taiwan Strait Tunnel Project

This article discusses briefly the major features of topography, geology, and crustal stability of the possible Taiwan Strait Tunnel Project (TST Project) area. Any one route of the conceived TST Project is larger than either of the English Channel Tunnel or the Seikan submarine Tunnel (Japan) by at least three times in length, and the topographic and geologic conditions are rather more complex and complicated than those of the former two projects. Moreover, the Taiwan Strait is a seismic risk area. Hence, the selection of the route of the submarine tunnel should take advantage of the favorable conditions in the topographic, geologic, seismologic, and other fields to avoid the unfavorable circumstances. At the end of the article, two supposed by comparable routes, especially the south one of TST Project are discussed.     

Geotechnical Aspects of Clayey Sediments Off Badagara on the Kerala Coast,India

Offshore geotechnical surveys form part of an integrated investigation to rejuvenate a decrepit minor port at Badagara, Kerala on the southwestern coast of India. The sediments typify a fluvio-marine milieu ranging from silty clay, sand, silty sand, sandy silt and clayey silt. Geotechnical and sedimentological studies of shallow cores reveal the geotechnical aspects besides the depositional history of the sediments. Downcore geotechnical variations and regressive coefficients based on their inter-relationships highlight diverse factorial inferences. X-Ray Diffraction data indicate the prominent clay type.

A comparative evaluation of the geotechnical characteristics of clayey sediments off Badagara, with similar studies along various sectors of the Kerala coast, both on land as well as in the near shore, is broadly attempted. Geotechnical studies carried out earlier on the uplifted Cochin marine clays provide comparative data for evaluating the possible variations between present day marine clayey sediments occurring along the Kerala coast and uplifted marine clays which, besides their gross variations in levels with respect to the present sea-level, also obviously relate to a much older depositional environment and provenance during probable Holocene times.     

一种承压堵漏新方法的实验研究

钻井过程中会遇到一些微裂缝地层,这往往要引起井漏.轻微的井漏可能只是耗费处理时间及成本,而严重井漏则可能会诱发诸如井塌、卡钻、井喷等一系列复杂情况或者事故,这将不仅影响钻井工作的效率,还带来巨大的经济损失.所以,堵漏是钻井过程中必须经常面对的问题,能够低成本地实现承压堵漏对于钻井井漏的处理就显得格外重要.使用新的材料/方法来提高地层的承压能力,即在抗高温聚磺钻井液中加入矿渣（KZ）,并在激活剂处理液中培养,配制出新型的堵漏钻井液体系.经过试验证明,加入KZ对钻井液常规性能的影响不大,不但能大幅度提高地层的承压能力,同时证明了激活剂对于激活KZ活性的可行性.对于抗高温聚磺钻井液,当KZ加量为30％、并且激活剂加量为30％时,试样的承压能力达到最大.此方法在一定程度上提高了微裂缝地层的承压能力.     

海口湾西海岸海滩沉积物与海滩稳定性分析

海滩是砂质海岸重要的沉积地貌单元,其沉积特征和稳定性对于海滩的开发利用与保护具有重要意义。本文根据海口湾西海岸海滩表层沉积物粒度数据,阐述了该区域海滩沉积物的时空分布规律,并通过对比滩面高程数据和水下地形数据分析了该区域的岸滩稳定性。结果表明：海口湾西海岸海滩处于滩面微蚀的轻度不稳定态,其岸外浅滩处于边坡侵蚀的轻度不稳定态。另外,根据波浪作用和泥沙来源进一步探讨了海口湾西海岸海滩沉积物动力机制及其海滩侵蚀的原因。     

Wave‐induced pore pressure in relation to ocean floor stability of cohesionless soils

One of the important geotechnical considerations for many engineering installations, such as pipelines and anchors, in an oceanic environment involving sand deposits is that of potential ocean floor instability due to the development of high pore pressures caused by the direct action of waves. This article presents a procedure for evaluating the magnitude and distribution of wave‐induced pore pressures in ocean floor deposits. The method takes into account the distribution of wave‐induced pore pressures in ocean floor deposits. The method takes into account the distribution of cyclic shear stresses in the soil profile as well as the important factor of pore‐pressure dissipation. The variation of properties within the soil profile can also be easily incorporated into the analytical procedure. The analysis provides the complete time history of pore‐pressure response and shows clearly that failure to include the pore‐pressure dissipation effects would lead to radically conservative design. The results also provide a basis for designing remedial measures, if required, to avert the development of high pore pressures and their deleterious effects.     

边坡可靠度分析的非侵入式随机有限元法

提出基于非侵入式随机有限元法的边坡可靠度分析方法,并编写计算程序NISFEM。采用有限元滑面应力法计算边坡安全系数,将Hermite随机多项式展开与SIGMA/W和SLOPE/W模块有机结合实现边坡可靠度非侵入式随机分析。根据随机多项式展开系数,给出边坡安全系数前4阶统计矩（均值、标准差、偏度和峰度）和Sobol指标解析表达式,并采用Sobol指标进行边坡可靠度参数敏感性分析。最后,以均质土坡可靠度问题为例,证明该方法在边坡可靠度分析中的有效性。结果表明,边坡可靠度分析的非侵入式随机有限元法能够有效地考虑边坡变形对边坡可靠度的影响,计算效率远远高于蒙特卡罗模拟方法（MCS）,是解决复杂边坡可靠度问题一种有效地分析手段;黏聚力和内摩擦角变异性对边坡安全系数前四阶统计矩具有明显的影响,重度变异性对安全系数前4阶统计矩几乎没有影响;抗剪强度参数间负相关性对边坡安全系数均值几乎没有影响,但对安全系数标准差、偏度和峰度均有明显的影响。此外,随着抗剪强度参数间负相关性的增加,边坡安全系数由近似正态分布逐渐变为明显的非正态分布。     

降雨过程中边坡临界滑动场

降雨入渗过程中孔隙水压力的升高与基质吸力的降低引起边坡稳定性的下降,是导致边坡滑塌的主要诱导因素。利用饱和-非饱和渗流有限元计算得到的孔隙水压力场,基于Fredlund提出的非饱和土抗剪强度理论,对边坡临界滑动场进行改进,提出可以考虑降雨过程的边坡临界滑动场数值模拟方法,能够方便、快速地计算出边坡局部、整体安全系数和相对应的临界滑动面在降雨过程中的变化历程。将该法用于一个典型均质边坡和一个非均质边坡在降雨过程中的稳定性计算,分析降雨持续时间、降雨强度和非饱和强度参数取值等因素对边坡稳定性的影响,并将计算结果与其他方法进行比较,结果表明临界滑动场方法能搜索任意形状最危险滑面,计算的安全系数合理。     

平面应变下结构性对黄土基坑稳定性的影响

鉴于以往很少考虑黄土结构性对基坑开挖的影响,针对目前普遍存在的基坑稳定性问题,开展室内平面应变条件下的真三轴试验,建立黄土结构性与强度之间关系,通过FISH语言将其引入到基坑开挖数值模拟中,分析结构性对基坑开挖的影响。研究表明,结构性参数与强度指标的变化规律与以往研究结果基本一致,表现为结构性参数与黏聚力呈双曲线关系,但参数略有不同,其受摩擦角的影响较小;结构性影响下基坑侧壁出现明显滑带,且此时连续墙的侧向位移大于不考虑其影响时的位移,坑底隆起量亦如此。研究结果对黄土基坑工程的设计和施工具有一定的指导意义。     

基于三维地质模型的排土场边坡整体稳定性探究

目前所广泛采用的平面应变分析手段,针对排土场边坡稳定性评价时有时会出现某剖面安全系数高于设定允许值,实际中却遭破坏的现象,除了常被提及的力学参数、本构模型等因素,排土场凹形夹持效应和凸形发散作用突出以及滑体端部效应的影响,是分析结果不能准确反映实际的根本原因之一。鉴于此,从平面高程数据、地质剖面、钻孔资料等条件,配合插值效果较为稳定的Kriging法对空间数据进行插值,建立排土场三维地质整体模型。然后,采用自编FISH语言接口程序,将地质模型导入FLAC3D中离散化并计算,较为简单、有效地实现三维地质建模、模型可视化、岩土工程计算分析三位一体功能,有效地克服了平面分析无法解决的上述问题。以攀钢兰尖铁矿尖山第7排土场为实,对其整体稳定性进行应力、应变分析,采用三维强度折减法计算排土场三维整体安全系数,与现场调查及平面计算结果较为一致。基于该模型的计算结果可以有效避免排土场空间效应和滑体端部效应,得到的排土场全域应力-应变规律,对露天矿排土场的生产管理,具有一定的指导意义。