红树植物木榄(Bruguiera gymnorrhiza)种群遗传变异与遗传分化的初步研究

研究了我国红树林分布区内3个木榄种群的遗传变异和遗传分化.在种水平和种群水平上,期望杂合度分别为0.293和0.268,观察杂合度分别为0.2745和0.2705.种群间遗传多样性为0.0830,表明总的遗传变异中有8.3%来自种群间.基因流较为顺畅,为3.34.     

胶州湾海水微表层粘度及其在海-气通量计算中的作用

物质海 -气通量计算新建议中将物质海 -气通量计算公式 F=K(CL- b Cg)中的 CL 用CL ( SML ) 代替。本文着重于对公式中质量迁移系数 K的讨论。在测定了海水微表层、次表层水粘度并同时测定了其它一些化学参量基础上 ,得出如下结论 :海水粘度与盐度、碱度有一定相关性 ;微表层与次表层海水的粘度变化小于 3%。因此 ,海水微表层效应影响 K值 ,与海水微表层效应影响物质浓度相比 ,可以不考虑。     

Effects of pulsed nutrient inputs on phytoplankton assemblage structure and blooms in an enclosed coastal area

The response of phytoplankton assemblage structure to terrestrial nutrient inputs was examined for the Gulf of Kalloni in the Northern Aegean Sea, a productive semi-enclosed coastal marine ecosystem. The study was focused on a typical annual cycle, and emphasis was placed on the comparative analysis between blooms developing after significant nutrient inputs from the watershed, and naturally occurring blooms. Baseline information was collected on a monthly basis from a network of stations located in the oligotrophic open sea and the interior and more productive part of the embayment. Intensive sampling was also carried out along a gradient in the vicinity of a river which was the most important source of freshwater and nutrient input for the Gulf. Phytoplankton assemblage structure was analyzed from 188 samples using diversity indices (Shannon and Average Taxonomic Distinctness), multivariate plotting methods (NMDS), multivariate statistics (PERMANOVA), and canonical correspondence analysis (CCA). Three characteristic assemblages were recognized: (1) an autumn assemblage developed under nutrient depleted conditions, having low diversity due to the dominance of two small diatoms, (2) a winter bloom of the potentially toxic species Pseudo-nitzschia calliantha occurring immediately after a nutrient peak and characterized by very low diversity, and (3) a naturally occurring early summer bloom of centric diatoms with relatively high diversity. The results of the study support the view that moderate nutrient inputs may have a beneficial effect on the functioning of coastal ecosystems, stimulating the taxonomic diversity through the growth of different taxonomic groups and taxa. On the other hand, a sudden pulse of high nutrient concentrations may greatly affect the natural succession of organisms, have a negative effect on the diversity through the dominance of a single species, and can increase the possibility of a harmful algal bloom development.     

Conservation Priority Index for Estuarine Fish (COPIEF)

Public awareness regarding environmental issues has increased in recent decades. The increasing number of impact assessment studies, management and conservation plans, as well as ecological monitoring studies, demand new and more efficient techniques. Indices are an important tool to aid biologists in these studies and should allow an easier comprehension of the data by managers, decision-makers and the general public. This study presents the first multi-metrical index able to establish a hierarchical ordination of the conservation priority of the estuarine fish species using 72 species from 16 estuarine systems (W and S coasts of Portugal). The index is composed of 10 metrics, comprising species life traits, distribution and population trends. The information needed to score each metric was gathered from the published literature and the index validation was done by external means. This methodology allowed the definition of those fish species most in need of conservation planning, and those less prone to extinction in Portuguese estuarine systems. The proposed index fills a gap in our knowledge and provides a useful tool to the scientific community and to the decision-makers, being a breakthrough in the field of conservation planning of estuarine fish species.     

Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA（RAPD）

Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well.     

椒江口潮间带大型底栖动物群落beta多样性分析

于2007年10月在椒江口共布设6条潮间带采样断面,每条断面在中潮带上区、中区、下区和低潮区各设1站位进行大型底栖动物采样。分析了不同采样站位数与大型底栖动物种数的关系,选用Wilson-Shmidabeta多样性测度方法,对以下3种空间环境梯度下大型底栖动物群落beta多样性进行了研究:(1)断面内垂直环境梯度;(2)河流到海洋的环境梯度;(3)河口两岸间的距离梯度。分析了不同采样站位数对群落beta多样性指数的影响,结果显示:(1)随着采样站位数的增加,物种数呈增加趋势但增速渐慢,同时beta多样性指数呈下降趋势;(2)不同的潮区间隔下的be-ta多样性指数均有显著差异;(3)在北岸和南岸,随着断面间距的增加beta多样性指数均呈升高趋势,而且与各断面的沉积物粒度、油类含量呈显著相关;(4)椒江口为喇叭形河口,随着河口两岸断面距离的增加beta多样性指数呈升高趋势。     

黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析

利用40个黄颡鱼微卫星分子标记对吉林省月亮湖野生黄颡鱼(YL,30尾)、四川野生黄颡鱼(SC,30尾)、黑龙江省哈尔滨市松花江段黄颡鱼(SH,18尾)、湖北省荆州市长湖野生黄颡鱼子代(YY,30尾)及其亲本(QQ,30尾)、天津市换新国家级原良种场黄颡鱼1号(TJ,30尾)6个黄颡鱼群体的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测,根据基因频率计算遗传相似系数和Nei氏标准遗传距离,x2检验估计Hardy-weinberg平衡,采用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源.同时,使用PHYLIP3.63软件绘制基于Nei氏标准遗传距离的UPGMA进化树.6个群体共检测到5042个扩增片段,长度在150-650bp,40个基因座扩增出等位基因数从1-8个不等,共计143个等位基因,平均每个基因座扩增得到3.575个等位基因.结果显示:(1)6个黄颡鱼群体的多态性指标均适中,PIC在0.00-0.79之间,平均值为0.40、0.37、0.35、0.39、0.35和0.19,有效等位基因数(Ae)1.00-5.44个不等,平均有效等位基因数依次为2.05、2.30、1.93、2.07、1.93和1.47,无偏期望杂合度(He)在0.00-0.83之间,平均值为0.45、0.46、0.36、0.46、0.41和0.23;(2)遗传相似系数YY与QQ遗传相似度最高(0.9947),TJ与SC遗传相似系数最低(0.4846),聚类结果与地理分布呈一定相关性.     

2004年秋季南海北部浮游植物组成及其数量分布特征

根据2004年9月18日—10月2日南海北部航次所获得的表层浮游植物群数据, 对其组成及数量分布进行了探讨分析。该次调查鉴定浮游植物共53属152种(含变种和变型), 分别属于硅藻门35属88种、甲藻门15属60种、蓝藻门1属2种及金藻门1属1种等; 优势种中分布较广泛的有柔弱伪菱形藻Pseudo-nitzschia delicatissima、反曲原甲藻Prorocentrum sigmoides、微小原甲藻P. mininum、锥状施克里普藻Scrippsiella trochoidea和红海束毛藻Trichodesmium erythraeum等; 浮游植物细胞丰度的数量范围为(0.11—23.61)×106cells•m-3, 呈现近岸区域高于远岸区域的平面分布格局, 最大值和最小值分别出现在海南岛东北部海域的E506站和海南岛东南部海域的B3站; 浮游植物物种丰富度也是近岸的站点较高, 以靠近珠江口E708站的51种为最多。硅藻类细胞丰度范围为(0.02—8.13)×106cells•m-3, 主要分布在高盐的外海水域, 其中产二甲基磺酸丙酯(DMSP)的小环藻Cyclotella spp.在多个站点出现; 甲藻类以近岸水域居多, 细胞丰度最高达15.67×106cells•m-3(E506站), 其中数量较多的赤潮种类主要有锥状施克里普藻、反曲原甲藻、微小原甲藻、环沟藻Gyrodinium spp.等; 固氮的红海束毛藻在调查海域广泛分布, 但主要集中于东部, 以E206站最多, 高达1.8×107cells•m-3。结合水文资料和其他层次的浮游植物资料进行对比分析, 结果表明, 红海束毛藻的分布与黑潮水的入侵存在一定关系, 其分布受水团和海流的影响。     

海南昌江沿岸海域石珊瑚的物种多样性及其分布

2009年3月,采用水下照相及摄像方法,对海南昌江沿岸海域的石珊瑚物种多样性、分布及覆盖度进行了调查.结合历史资料,共发现石珊瑚10科15属28种和1未定种.其中鹿角珊瑚属（Acropora）、角蜂巢珊瑚属（Favites）、叶状珊瑚属（Lobophyllia）等为优势类群,常见分布珊瑚种类有角孔珊瑚（Gonioporasp.）、标准蜂巢珊瑚（Favia speciosa）、五边角蜂巢珊瑚（Favites pentagona）、多孔鹿角珊瑚（Acropora millepora）、十字牡丹珊瑚（Pavona decussata）、伞房叶状珊瑚（Lobophyllia corymbosa）等.石珊瑚主要分布于海尾镇三联村以南海域,覆盖率最高为12%.本次调查在海南昌江沿岸海域记录的珊瑚种类数多于以前的记录,但少于海南南岸的三亚鹿回头,也少于北部的广西涠洲岛、广东徐闻、香港等海域,随着调查次数的增加,预计会有更多的种类被发现.该海域是北部湾珊瑚幼虫交流的中间过渡区,对珊瑚礁的保护与恢复具有重要意义.     

Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer

The spatial distribution of ammonia-oxidizing Betaproteobacteria ( β AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary.Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006.The abundance of β AOB ranged from 1.87 × 10 5 to 3.53 × 10 5 cells/g of sediment.β AOB abundance did not present a negative correlation with salinity,whereas salinity was implicated as the primary factor affecting nitrification rates.The DGGE profiles of PCR-amplified amo A gene fragments revealed that the β AOB community structure of sample S2 separated from other samples at the level of 40% similarity.The variations in composition of β AOB were significantly correlated with the salinity,temperature,absorption ability of sediments and TOC. The statistical analysis indicates that the β AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity.Considering the good correlation between β AOB abundance and nitrification estimates,the abundance and diversity of β AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.