粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。     

Using live vegetation volume to analyze the effects of plot Pinus massoniana Lamb on water and soil conservation under natural rainfall events

The 3-D spatial distributions of vegetation are of great significance for water and soil conservation but are rarely concerned in literatures. The live vegetation volume （LVV） was used to relate to water/soil loss under 144 natural erosive rainfall events from 2007 to 2010 in a typical water-eroded area of southern China. Quadratic polynomial regression models were established for five pure tree （Pinus massoniana Lamb） plots between LVV and water （rtmoff）/soil conservation effects （RE/SE）. RE/SE corresponds to the ratios of runoff depth/soil loss of the pure tree plots to that of the control plot under each rainfall event. Increasing LVV exhibits descending （DS）, descending-ascending （DA）, ascending-descending （AD）, and ascending （AS） trends in the LVV-RE and LVV-SE curves. The effects of soil conservation on the plots were generally more noticeable than the effects of water conservation, and most of the RE and SE values reflected the positive effects of water and soil conservation. The effects were mainly positive under heavy rains （e.g., rainfall erosivity, R = 140 MJ mm ha-l h, maximum 30 min intensity, I30 = 16 mm h-l）, whereas the effects were mainly negative under light rains （e.g., R = 45 MJ mm ha-1 h, I30 = 8 mm h-l）. The trees＇ water/soil conservation effects notably transformed when rainfall erosivity and intensity were lower than the positive or negative effects to a certain threshold. About 50% rainfall events led to obvious transform effects when LVVs were near 0.5 or 0.6. These results are able to aid in the decision making on the forest reconstruction in water-eroded areas.     

Water Quality Impacts of Climate and Land Use Changes in Southeastern Pennsylvania*

This study investigates potential changes in nitrogen and phosphorus loads under a warmer and wetter climate, urban growth, and combined changes in the Conestoga River Basin and its five subbasins in southeastern Pennsylvania. A GIS‐based hydrochemical model was employed for assessing the sensitivity of the basins to the projected changes in 2030. Under the HadCM2 climate change scenario, mean annual nitrogen and phosphorus loads are expected to increase, with great increases in spring but slight decreases in fall primarily because of changes in monthly precipitation. When climate change and urbanization occur concurrently, mean annual nitrogen loads further increase by 50% in the most urbanizing subbasin. Point source nitrogen control could mitigate negative effects of climate and land use changes, reducing mean annual nitrogen loads to the contemporary baseline level.     

A smoothed cap model for variably saturated soils and its robust numerical implementation

This paper deals with the numerical implementation of a cap model for unsaturated soils. It provides a brief review of existing cap model approaches, based on which an improved model formulated in terms of generalised effective stress and matric suction is derived and described in detail. Although the proposed model is a multisurface plasticity model, it can efficiently be implemented using only single‐surface projections because of the smoothness of the model, which is obtained by construction. Numerical algorithms are provided for these single‐surface stress projections, using a single‐equation approach whenever possible. The robustness of the utilised single‐equation approaches is enhanced by proposing problem‐fitted start‐up procedures based on investigations of the nonlinear projection equations. A comparison of the model response with extensive material test data is used to validate the model and to demonstrate the robust application of the approach to silty sands and low to medium plasticity clays. Copyright © 2015 John Wiley & Sons, Ltd.     

岩体工程质量分级应用研究

岩体质量评价采用的测试技术包括地质勘察、弹性波检测和室内岩石力学试验。结合水布垭水利枢纽大坝建基岩体工程质量评价实例,对岩体工程质量分级的应用进行了研究。     

Vegetation patterns and species–environment relationships in the Gurbantunggut Desert of China

The Gurbantunggut Desert is the largest stable and semi-stable sand desert in China, yet few data exist on vegetation pattern and species-environment relationships for these diverse desert landscapes. The sand dunes of the survey area are mainly of the longitudinal form from north to south, but checkerboard-shaped and honeycomb-shaped forms are also present, with the height of 10–50 m. We measured vegetation and soil data on north–south transects and compared them with vegetation and soil data on east–west transects. Analysis revealed that the varying trend and strength of the species diversity, dominance and cover of the plant communities in the longitudinal and transverse directions across the landscape are significantly different. The results of CCA ordination show that the microhabitats of soil organic matter (OM), soil salts (TS), sorting index (σ), topsoil water-content (SM) and pH relate to the differences in vegetation observed as differences in species assemblage from salt-intolerant ephemerals, micro-subshrubs and subshrubs to salt-tolerant shrubs and micro-arbors. The terrain (alt.) and soil texture (the contents of Mz and φ1) affect the spatial differentiation of many species. However, this spatial differentiation is not so marked on transects running longitudinally with the landscape, in the same direction as the dunes. The species of the desert vegetation have formed three assemblages under the action of habitat gradients, relating to three sections running transversely across the landscape, at right angles to the direction of the dune crests. In the mid-east section of the study area the topography is higher, with sand-lands or dune-slopes with coarse particles. Here the dominant vegetation comprises shrubs and subshrubs of Seriphidium santolium and Ephedra distachya, with large numbers of ephemeral and ephemeroid plants of Senecio subdentatus, and Carex physodes in spring and summer. On the soil of the dune-slopes in the mid-west of the study area, with coarse particles and abundant TK, the plant assemblage of Haloxylon persicum, Soranthus meyeri and Agriophyllum squarrosum is developed. The species composition in the east marginal belt of the study area has similar characteristics to the mid-west section. There is no corresponding section in the north–south transects (except for the north and south margins). This is because the habitats of most plants are located in the middle of the microhabitat gradients in the north–south direction in the desert.     

三江源地区土壤侵蚀特点

三江源地区地域辽阔,土壤侵蚀复杂多样.近年来土壤侵蚀加剧,生态环境严重退化.以2000年土壤侵蚀调查的数据成果为基数.并与更新后的2005年数据成果比较,进行土壤侵蚀动态变化分析.对三江源地区不同类型土壤侵蚀区的土壤侵蚀现状做了较全面的分区评价.同时对作为典型区域的黄河源头区12个县的2000年1:10万Landsat 5 TIM假彩色合成影像,通过野外调查建它解译标志来分析该区植被覆盖状况,对该区典型区域的土壤侵蚀现状做了定量评价.根据评价结果,对三江源地区土壤侵蚀的发展趋势做了预测.     

Geochemistry of environmentally sensitive trace elements in Permian coals from the Huainan coalfield, Anhui, China

To study the geochemical characteristics of 11 environmentally sensitive trace elements in the coals of the Permian Period from the Huainan coalfield, Anhui province, China, borehole samples of 336 coals, two partings, and four roof and floor mudstones were collected from mineable coal seams. Major elements and selected trace elements were determined by inductively coupled plasma optical emission spectrometry (ICP-OES), inductively coupled plasma mass spectrometry (ICP-MS), and hydride generation atomic absorption spectrometry (HAAS). The depositional environment, abundances, distribution, and modes of occurrence of trace elements were investigated. Results show that clay and carbonate minerals are the principal inorganic constituents in the coals. A lower deltaic plain, where fluvial channel systems developed successively, was the likely depositional environment of the Permian coals in the Huainan coalfield. All major elements have wider variation ranges than those of Chinese coals except for Mg and Fe. The contents of Cr, Co, Ni, and Se are higher than their averages for Chinese coals and world coals. Vertical variations of trace elements in different formations are not significant except for B and Ba. Certain roof and partings are distinctly higher in trace elements than underlying coal bench samples. The modes of occurrence of trace elements vary in different coal seams as a result of different coal-forming environments. Vanadium, Cr, and Th are associated with aluminosilicate minerals, Ba with carbonate minerals, and Cu, Zn, As, Se, and Pb mainly with sulfide minerals.     

海洋环境观测技术研究进展

回顾了近年来海洋观测技术发展的基本情况和主要成果,同时指出了海洋观测技术的局限性,与数值模拟技术相结合的必要性,以及加强发展海啸、风暴潮和孤立内波等灾害性强海洋动力过程的监测技术和预警研究的迫切性。