Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

Current-induced scour beneath initially elevated subsea pipelines

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.     

Simulation of paleotectonic stress fields and quantitative prediction of multi-period fractures in shale reservoirs: A case study of the Niutitang Formation in the Lower Cambrian in the Cen'gong block,South China

Reservoirs where tectonic fractures significantly impact fluid flow are widespread. Industrial-level shale gas production has been established from the Lower Cambrian Niutitang Formation in the Cen'gong block, South China; the practice of exploration and development of shale gas in the Cen'gong block shows that the abundance of gas in different layers and wells is closely related to the degree of development of fractures. In this study, the data obtained from outcrop, cores, and logs were used to determine the developmental characteristics of such tectonic fractures. By doing an analysis of structural evolution, acoustic emission, burial history, logging evaluation, seismic inversion, and rock mechanics tests, 3-D heterogeneous geomechanical models were established by using a finite element method (FEM) stress analysis approach to simulate paleotectonic stress fields during the Late Hercynian—Early Indo-Chinese and Middle-Late Yanshanian periods. The effects of faulting, folding, and variations of mechanical parameters on the development of fractures could then be identified. A fracture density calculation model was established to determine the quantitative development of fractures in different stages and layers. Favorable areas for shale gas exploration were determined by examining the relationship between fracture density and gas content of three wells. The simulation results indicate the magnitude of minimum principal stress during the Late Hercynian — Early Indo-Chinese period within the Cen'gong block is −100 ∼ −110 MPa with a direction of SE-NW (140°–320°), and the magnitude of the maximum principal stress during the Middle-Late Yanshanian period within the Cen'gong block is 150–170 MPa with a direction of NNW-SSE (345°–165°). During the Late Hercynian — Early Indo-Chinese period, the mechanical parameters and faults play an important role in the development of fractures, and fractures at the downthrown side of the fault are more developed than those at the uplifted side; folding plays an important role in the development of fractures in the Middle-Late Yanshanian period, and faulting is a secondary control. This 3-D heterogeneous geomechanical modelling method and fracture density calculation modelling are not only significant for prediction of shale fractures in complex structural areas, but also have a practical significance for the prediction of other reservoir fractures.     

Molecular iodine (I2) emission from two Laminaria species (Phaeophyceae) and impact of irradiance and temperature on I2 emission into air and iodide release into seawater from Laminaria digitata

Kelps of the genus Laminaria accumulate iodine at high concentrations, but the iodine retaining capacity can be affected by emersion and physiological stress. In this study, I₂ emission into the atmosphere from Laminaria digitata and Laminaria hyperborea was compared under controlled low irradiances and temperatures. The two species exhibited different I₂ emission rates as blades of L. digitata emitted I₂ at rates five times higher than those from newly-grown blades (current growth season) of L. hyperborea. I₂ emission was not detectable from old blades (previous growth season) of L. hyperborea. Additionally, effects of irradiance and temperature on both I₂ emission into air and net I⁻ release into seawater where assessed for L. digitata while monitoring photo-physiological parameters as stress indicators. Irradiances between 30 and 120 μmol photons m⁻² s⁻¹ had only marginal effects on both I₂ emission and I⁻ release rates, but physiological stress, indicated by photoinhibition, was observed. The results suggest that the irradiances applied here were not stressful enough to impact on the iodine release. By contrast, at elevated temperatures (20 °C), photoinhibition was accompanied by an increase in I₂ emission rates, but net I⁻ release rates remained similar at 10–20 °C. High I₂ emission rates into air and I⁻ release into seawater observed from L. digitata underpin the fundamental function of this kelp as mediator of coastal iodine fluxes.     

急性低氧胁迫对卵形鲳鲹选育群体血液生化指标的影响

本文研究了水环境急性低氧胁迫对卵形鲳鲹Trachinotus ovatus选育群体血液生化指标的影响。实验设计了急性低氧试验组和对照组,每组3个平行。结果显示,急性低氧胁迫后选育群体的血清离子含量与对照组相比都有不同程度的升高,其中钠、氯、钙浓度与对照组相比差异显著(p0.05),铁浓度极显著高于对照组(p0.01);血清蛋白、尿酸、肌酐、血脂、血糖等指标的差异较大,其中尿酸、肌酐极显著高于对照组(p0.01),总蛋白、总胆固醇显著低于对照组(p0.05);血清中肌酸激酶含量极显著高于对照组(p0.01),肌酸激酶同工酶显著高于对照组(p0.05),γ-谷氨酰转肽酶含量显著低于对照组(p0.05);其他指标均有不同程度的升高或降低,但差异不显著。研究表明,急性低氧胁迫造成卵形鲳鲹鳃、肾、心脏和肝脏损伤,免疫力下降。     

围海造陆工程泄水口悬浮物扩散规律分析

掌握围海造陆工程泄水口悬浮物扩散规律,对保护海洋水环境质量具有重要意义。选取围海造陆工程泄水口为研究对象,基于泥沙对流扩散方程,推求出泄水口悬浮物扩散平面二维分析解表达式。开展现场观测,确定泄水口源强取值,并对泄水口悬浮物扩散进行理论计算和分析。研究结果显示,围海造陆工程施工后期,泄水口悬浮物流失非常严重。泄水口附近水域出现的最大悬浮物浓度主要由源强的大小来决定,悬浮物扩散范围主要由流速的大小来控制。     

波致液化粉质土有效应力变化的试验数据分析

海床不稳定性的现象很多都是海底液化引起的。根据之前的研究,粉土在液化之后有效应力会依然存在,关于波浪作用下海床液化之后有效应力变化的研究很少。采用波浪水槽实验,在未液化和液化2种情况下,分别施加不同波高的波浪,对底床各层位土体孔隙水压力进行采集,并对比研究。实验结果表明,液化后有效应力的相对值相对于液化前有很明显的减小,并且在深度上表现出显著的差异性,这种差异性随着波高的增大而减小。当相同深度处同一种波高作用一定时间时,有效应力会出现极大值,然后有效应力会减小。     

Delineation of Potential Seismic Source Regions of Moderately Strong Earthquakes Without Seismogenic Tectonics

In this paper,approaches are developed to delineate the potential seismic source regions of moderately strong earthquakes that do not have clear seismotectonic settings.Based on comprehensive analysis of regional tectonic backgrounds and seismicity,the data,such as isoseisrnals,spatial distribution of after shocks,regional tectonic stress field,and focal mechanisms,are employed for the delineation of the potential seismic source regions.The reliability of such potential seismic source regions is also discussed.     

基于剖分的中尺度海洋温度场三维显示技术

针对网格化和数据量庞大的中尺度海洋标量场数据在其三维可视化中表现出来的构造效率较低和表达连续性效果较差的问题,以剖分重构为核心思想,采用三线性插值算法对数据进行加密处理,基于不同角度平面对温度场进行剖分和色彩映射,最后采用OpenSceneGraph图像渲染引擎的颜色、光照、透明度和回调等渲染技术展现更为真实的、科学的动态三维温度场,提供一种简洁、高效、科学的中尺度海洋温度场表达方法。     

Evaluation of Effective Depth of PVD Improvement in Soft Clay Deposit: A Field Case Study

This article presents a case history of determination of effective depth of prefabricated vertical drains (PVDs) under embankment loading on a very soft clay deposit in central China, near Jiujiang, Jiangxi Province. The height of the embankment was 5.3 m and construction time was about one year. The PVDs were installed to a depth of 8.5 m at a spacing of 1.5 m in a triangular pattern. Field observations and the finite element method (FEM) were employed to analyze the performance of the soft deposit during embankment construction. The influential depth of the embankment loading was evaluated based on settlement, excess pore pressure, and stress increase in subsoil, both from the observed data and FEM analysis. The effective PVD depth was determined in the following ways: (1) the depth of 5% subsoil settlement of surface settlement; (2) vertical stress increase in subsoil of 25% in-situ stress; and (3) consolidation time/PVD depth relation by FEM. Based on the analysis, the effective depth of PVDs was determined to be between 10 and 12.8 m for this field case.