Assessment of Fatigue Strength of An Offshore Floating Production and Storage Unit

The procedure of assessment of structural fatigue strength of an offshore floating production and storage and offloading unit(FPSO) in this paper. The emphasis is placed on the long-term prediction of wave induced loading, the refined finite element model for hot spot stress calculation, the combination of stress components, and fatigue damage assessment based on S-N curve.     

Structure of the Malpelo Ridge (Colombia) from seismic and gravity modelling

Wide-angle and multichannel seismic data collected on the Malpelo Ridge provide an image of the deep structure of the ridge and new insights on its emplacement and tectonic history. The crustal structure of the Malpelo Ridge shows a 14 km thick asymmetric crustal root with a smooth transition to the oceanic basin southeastward, whereas the transition is abrupt beneath its northwestern flank. Crustal thickening is mainly related to the thickening of the lower crust, which exhibits velocities from 6.5 to 7.4 km/s. The deep structure is consistent with emplacement at an active spreading axis under a hotspot like the present-day Galapagos Hotspot on the Cocos-Nazca Spreading Centre. Our results favour the hypothesis that the Malpelo Ridge was formerly a continuation of the Cocos Ridge, emplaced simultaneously with the Carnegie Ridge at the Cocos-Nazca Spreading Centre, from which it was separated and subsequently drifted southward relative to the Cocos Ridge due to differential motion along the dextral strike-slip Panama Fracture Zone. The steep faulted northern flank of the Malpelo Ridge and the counterpart steep and faulted southern flank of Regina Ridge are possibly related to a rifting phase that resulted in the Coiba Microplate’s separation from the Nazca Plate along the Sandra Rift.     

套式PCR检测斑节对虾白斑症病毒(WSSV)

解剖患白斑症斑节对虾的鳃组织 ,制备成不同稀释倍数的模板 ,对其进行白斑症病毒 (WSSV)的 PCR扩增 ,结果显示所建立的套式 PCR的灵敏度大约为一步 PCR的 10 4 倍 ;对感染病毒后不同时期的斑节对虾进行 PCR检测 ,发现感染早期经一步 PCR检测为 WSSV阴性的样品 ,套式 PCR的检测结果为阳性 ;对发病虾塘中的几种甲壳类动物进行 PCR检测 ,发现经一步 PCR检测为阴性的长臂虾、秉氏厚蟹和褶痕相手蟹等宿主 ,套式 PCR的检测结果为阳性。表明所建立的 WSSV套式 PCR检测法较普通的一步 PCR有更大的应用价值和意义     

营养盐和微量元素与黄骅赤潮的相关性

本文主要通过１９８９～１９９２年河北省黄骅市沿海一带赤潮水质监测的结果，探讨赤潮发生期间营养盐及微量元素的含量变化与赤潮的关系。结果表明，营养盐的富集导致赤潮藻类的快速增殖，微量元素Ｆｅ和Ｍｎ的过量又是赤潮形成的诱发因子。     

Gravity and multichannel seismic reflection constraints on the lithospheric structure of the Canary Swell

Deep penetrating multichannel seismic reflection and gravity data have been used to study the lithospheric structure of the Canary Swell. The seismic reflection data show the transition from undisturbed Jurassic oceanic crust, away from the Canary Islands, to an area of ocean crust strongly modified by the Canary volcanism (ACV). Outside the ACV the seismic records image a well layered sedimentary cover, underlined by a bright reflection from the top of the igneous basement and also relatively continuous reflections from the base of the crust. In the ACV the definition of the boundary between sedimentary cover and igneous basement and the crust-mantle boundary remains very loose. Two-dimensional gravity modelling in the area outside the influence of the Canary volcanism, where the reflection data constrain the structure of the ocean crust, suggests a thinning of the lithosphere. The base of the lithosphere rises from 100 km, about 400 km west of the ACV, to 80 km at the outer limit of the ACV. In addition, depth conversion of the seismic reflection data and unloading of the sediments indicate the presence of a regional depth anomaly of an extension similar to the lithospheric thinning inferred from gravity modelling. The depth anomaly associated with the swell, after correction for sediment weight, is about 500 m. We interpret the lithospheric thinning as an indication of reheating of old Mesozoic lithosphere beneath the Canary Basin and along with the depth anomaly as indicating a thermal rejuvenation of the lithosphere. We suggest that the most likely origin for the Canary Islands is a hot spot.     

建立白斑综合症病毒在日本对虾体内潜伏性感染的方法研究

在27℃，pH8．0，盐度33条件下，用套式PCR方法和王证点杂交方法对日本对虾(Penaeus japonicus)体内潜伏性感染WSSV的方法进行了研究。对健康日本对虾注射不同稀释倍数的白斑综合症病毒粗提液，死亡率统计显示注射10^4，10^6倍稀释液组的日本对虾死亡率明显低于10，10^2，10^3倍组，结合套式PCR方法和斑点杂交方法进行病毒检测结果发现，仅10，10^2倍稀释液组斑点杂交和一步PCR结果呈阳性；10^3，10^4，10^6倍稀释液组经二步PCR检测为阳性、斑点杂交呈阴性，该结果为建立或界定WSSV在日本对虾体内的潜伏性感染提供了方法学上的依据。     

Parametric Equation of Stress Concentration Factor for Circular X-Joints Under Axial Loads

In engineering practice,tubular X-joints have been widely used in offshore structures.The fatigue failure of tubular X-joints in offshore engineering is mainly caused by axial tensile stress.In this study,the stress concentration factor distribution along the weld toe in the hot spot stress region for tubular X-joints subject to axial loads have been analyzed by use of finite element method.Through numerical analysis,it has been found that the peak stress concentration factor is located at the saddle position.Thereafter,80 models have been analyzed,and the effect of the geometric parameters of a tubular X-joint on the stress concentration factor has been investigated.Based on the experimental values of the numerical stress concentration factor,a parametric equation to calculate the stress concentration factor of tubular X-joints has been proposed.The accuracy of this equation has been verified against the requirement of the Fatigue Guidance Review Panel,and the proposed equation is found capable of producing reasonably accurate stress concentration factor values for tubular X-joints subject to axial loads.     

对虾白斑杆状病毒基因中的小顺反子及其表达

通过对虾白斑杆状病毒DNA文库和cDNA文库的碱基序列测定和分析,发现对虾白斑杆状病毒的一个开放阅读框的前导序列中含有一个小顺反子,这个小顺反子较多使用对虾基因和白斑杆状病毒基因的稀有密码子.试验表明在该基因的前导序列中存在或不存在小顺反子时,该基因都可在大肠杆菌中诱导表达,但两者的表达量不同.小顺反子可能对基因的表达具有调控作用.     

An Improved Formulation for the Ultimate Static Strength of Tubular Joints subjected to Combined Loads

"The effect of interaction of loads on the ultimate static strength of tubular joints of offshore fixed platforms, is a practical problem. But there is still absence of rigorous theory to explain available experimental data and empirical criteria for the static strength of tubular joints. The idea of yield at hot spot of tubular joints is introduced in this paper. The interaction equations of plastic capacity for the tubular joints under combined loads (two and three different kinds) are derived. Thereafter the Yura's test data and empirical criteria of ultimate static strength for the tubular joints can be explained. The idea of classification of category of loads in accordance with experimental data and the present theory is suggested. Finally, the improved ultimate capacity equations for tubular joints are recommended. The physical significance of the coefficient of plastic reservation Qp is discussed.     

中国对虾甲壳褐斑病的扫描电镜研究

本文报导了病虾溃疡甲壳的扫描电镜观察结果。由于细菌的侵蚀,甲壳有不同程度的溃疡病变。由甲酚类物质组成的表壳层结构普遍受损,呈剥离状。外壳层和含钙内壳层也有大面积的深度损害。刚毛断裂的结构显示,刚毛由内轴和外鞘组成,外鞘厚度约为1μm,内轴为实心,厚度约为5μm,轴鞘结构之间有一宽度为0.5μm的间隙。