人工养殖点带石斑鱼弧菌病病原菌的分离及鉴定

广东、海南两省多个海水网箱养殖场养殖的点带石斑鱼(Epinephelus malabaricus)发生严重的弧菌病,从海南三亚和广东深圳的发病鱼病灶上分离到2株致病性细菌VAL02和VAL00,经人工回接感染实验证明这2株菌引起点带石斑鱼弧菌病,VAL02对点带石斑鱼苗的半致死量是2.25×10~5 cfu/g。经形态及生理生化特征分析,鉴定出VAL02及VAL00均为溶藻弧菌。应用药敏纸片法研究了22种化学疗剂对VAL02及溶藻弧菌标准株的生长抑制作用,发现VAL02对氯霉素、氟哌酸、卡那霉素和乙酰螺旋霉素等产生耐药性,对复方新诺明和环丙沙星等敏感。     

A Study on the Dynamic Analysis of A Tracked Vehicle for Ocean Mining on the Deep Seabed

1 .IntroductionOnthedeepseabed ,30 0 0～ 50 0 0mbelowthePacificOcean ,manganesenodulesaresolidifiedandspread ,andmanycountriesintheworldarestudyinghowtominethem .AccordingtoYamazakietal.( 1 998)andAmannetal.( 1 991 ) ,theenvironmentofthedeepseabedisdifferentfromthedrylandinmanyways .Inparticular,thesurfaceoftheseabedisverysoft.Inordertoensuretheperformanceofrunningofamanganesenodulemineronsuchasoftsurface ,thetrackedvehiclemaybeapplied .Ac cordingtoHongandChoi ( 2 0 0 1 ) ,astudyisbeingdone…     

三对角系统的最佳向后扰动分析

用一种新方法研究了三对角方程组及对称三对角方程组计算解的最佳向后扰动分析。由于这两类方程组的系数矩阵具有特殊结构 ,因而本文结果不能从已有结果直接导出。所用方法亦可用于研究 KKT及 SQD等结构线性系统的最佳向后扰动分析。     

三苯基氯化锡对扁藻细胞超微结构的影响

于１９９２年４月－１９９２年６月以扁藻为材料进行半抑制深度三苯基氯化锡处理７２ｈ实验，用透射电镜观察观察中毒状态扁藻细胞超微结构的变化。结果表明，线粒体发生水肿，内脊局部瓦解，基质内出现由双层膜包围的同心质膜轮；叶绿体内光合片层的网状结构被破坏，光合片层上下重叠成厚块状；蛋白核中央髓部肿胀，淀粉鞘破裂成多角形淀粉板，并分散在髓部周围；叶绿体基质中出现大量淀粉粒，眼点因受淀粉粒挤压而由藻体中下部移至     

海洋环境数据业务化处理与应用原型系统

简单介绍了原型系统的概念,并重点介绍了在原型开发模式下,建立的海洋环境数据业务化处理原型系统的数据处理流程、总体结构和主要功能。     

Topographic Effect of Izu Ridge on the Distribution of the North Pacific Intermediate Water South of Japan

The topographic effect of the Izu Ridge on the horizontal distribution of the North Pacific Intermediate Water (NPIW) south of Japan has been studied using observational data obtained by the Seisui-Maru of Mie University (Mie Univ. data) and those compiled by Japan Oceanographic Data Center (JODC data). Both data sets show that water of salinity less than 34.1 psu on potential density () surface of 26.8 is confined to the eastern side of the Izu Ridge, while water of salinity less than 34.2 psu is confined to the southern area over the Izu Ridge at a depth greater than 2000 m and to the southeastern area in the Shikoku Basin. It is also shown by T-S analysis of Mie Univ. data over the Izu Ridge that water of salinity less than 34.2 psu dominates south of 30°N, where the depth of the Izu Ridge is deeper than 2000 m and NPIW can intrude westward over the Izu Ridge. JODC data reveal that relatively large standard deviations of the salinity on surface of 26.7, 26.8 and 26.9 are detected along the mean current path of the Kuroshio and the Kuroshio Extension. Almost all of the standard deviations are less than 0.05 psu in other area with the NPIW, which shows that the time variation in the salinity can be neglected. This observational evidence shows that the topographic effect of the Izu Ridge on the horizontal distribution of the NPIW, which is formed east of 145°E by the mixing of the Kuroshio water and the Oyashio water, is prominent north of 30°N with a depth shallower than 2000 m.     

Rejection of Intracoelomic Invading Material by Leptosynapta inhaerens (Echinodermata: Holothuroida): A Process of Ecological Significance?

Abstract. The synaptid holothuroid Leptosynapta inhaerens has the ability to expel unwanted particles that enter its body cavity. Intracoelomic particles ( viz. experimentally injected carmine particles) are trapped either by a coelom-produced mucoid net or by specialized organs (the vibratile urnae) that occur in bands in some interradial areas. Whatever the trapping method, particles are incorporated into dense mucoid masses that move towards the posterior part of the body cavity, in the vicinity of the rectum. These aggregates then cross the rectal wall through rectal pores, mix with the faeces, and are eventually incorporated into the wall of the synaptid burrow. Clearance of foreign particles requires 2 to 3 days. The coelom-cleaning system of synaptids appears to be the most effective within the class Holothuroidea , being well designed for endofaunal organisms whose walls are delicate and easily rupture.     

石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征

病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s^-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s^-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s^-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。     

台风预报系统

本文介绍的是一项在微机上实现的台风预报专家系统。该系统可进行台风气候查询、台风路径检索、台风特例查询，并能利用相似加权法制作出１２－７２小时台风路径和中心强度预报，对异常路径台风也可提供一定的诊断预报判据。本系统已在军内外一些气象业务单位得到应用，实践证明它是一种简便有效的台风预报工具。