琼胶降解菌F-6筛选、培养条件及对条斑紫菜(Porphyra yezoensis)细胞解离作用的研究

利用琼胶作为唯一的碳源从青岛太平角海域的海水和红藻样品中筛选分离得到一株高产琼胶酶的海洋菌F-6,对其最适的产酶条件,利用琼胶酶分离条斑紫菜的原生质体和原生质体的培养进行研究。结果表明,通过单次单因子和正交实验确定琼胶降解菌株F-6最适产酶培养基配方为(W/V):琼胶0.7%;酵母粉0.3%;蛋白胨0.5%;NaCl2.0%;K2HPO40.1%;CaCl20.02%;MgSO4·7H2O0.05%;FeSO4·7H2O0.002%;起始pH=7.5,最适的发酵产酶条件为:26℃培养36h。经条件优化后,粗酶液的酶活高达631.6U/ml。发酵液经过6000g离心30min得到粗酶液,粗酶液经过8k分离膜超滤,加入1mol/L渗透剂,0.22μm滤膜过滤除菌后降解条斑紫菜组织块,成功地分离得到大量的紫菜原生质体,其产率为3×106个原生质体/g新鲜紫菜组织。原生质体经初步培养发育成愈伤组织,其成活率为60%。     

极地产适冷脂肪酶菌株的酶学性质研究

从南北极环境样品中分离到7株产脂肪酶的细菌,经16SrDNA序列分析表明,这些细菌分别属于假交替单胞菌属（Pseudoalteromonas）和嗜冷杆菌属（Psychrobacter）．采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在30～40cC、最适作用pH值在7—8之间,在低温下能保持较高的剩余活力,对热敏感,属于适冷脂肪酶．其中假交替单胞菌（Psychrobacter sp．7342）所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点．该菌株能利用多种单一氮源和碳源产酶,最适产酶温度为25℃．在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为：蛋白胨和淀粉含量各为1．33％,酵母膏含量为0．3％,温度为25℃．     

利用叶绿素荧光技术揭示光照、温度和盐度对鼠尾藻嫩芽的影响

采用鼠尾藻嫩芽为实验材料, 研究了不同光强（25～220 μmol/（m2·s））、温度（5～34℃）和盐度（0～60）对其叶绿素荧光参数的影响。测定的叶绿素荧光参数包括光系统II 最大荧光产量（Fv/Fm）、最大潜在相对电子传递速率（RrET,max）、快速光曲线的初始斜率等。结果表明： （1）鼠尾藻嫩芽易受强光、高...     

枯竭型油气藏储气库射孔完井技术

枯竭型油气藏储气库一般采用套管射孔完井方式,注、采气需要“缓进大出”,要求储层与井筒良好沟通.针对枯竭型油气藏储气库射孔完井工艺复杂、要求高、地层保护难度大等特点,阐述了射孔方式及工艺优选、射孔参数优化、射孔压实带改造技术、多级负压起爆技术等内容.该项射孔完井技术在中石化文96储气库13口井成功应用,取得了良好的效果.     

现代海洋地质学及其发展

近几十年来,海洋的重要性愈来愈明显,海洋地质学作为海洋科学中最活跃的一门科学也取得了很大的发展。对海洋地质学的研究内容、意义以及当前发展的现状和趋势作了介绍。     

中国龙虾生长特点及促生长途径的探讨

蜕壳后新甲壳完全硬化之前是中国龙虾(Panulirus stimpsoni)个体蜕壳生长过程的延续,对中国龙虾正常快速生长具有重要意义。环境因子、营养条件对中国龙虾的蜕壳与生长具有显著影响。24-27℃是中国龙虾蜕壳生长的适温范围;盐度24-32适宜中国龙虾生长,最适生长盐度是28;在6种试验饵料中翡翠贻贝饲养中国龙虾效果最好,其次是杂虾、杂蟹、牡蛎,投喂杂鱼的中国龙虾蜕壳增长率和阶段生长率都很低;龙虾饲料中添加蜕壳素能够縮短蜕壳周期,实现蜕壳率和蜕壳增长率的同步增长。摘除眼柄也能促进中国龙虾的蜕壳与生长,电刺激等外源刺激则只能促进蜕壳,而不能加速中国龙虾的生长,甚至可能产生负面影响。中国龙虾生长存在种内性别间的差异,同等条件下雄性个体的蜕壳增长率高于雌性个体。     

Latitudinal and local variations of the life history characteristics of the thalassinidean decapod, Upogebia yokoyai: A hypothesis based on trophic conditions

Mean total length of the eldest cohort (MTLe) of the thalassinidean decapod, Upogebia yokoyai sampled at 16 sites showed a latitudinal variation from south-west islands of the Ryukyu Archipelago (24° N) to the Seto-Inland Sea of Japan (34° N). Local variations were however also found from sites in the same latitudes. The level of the organic content in each habitat showed a positive correlation with MTLe. We hypothesized that MTLe is strongly affected by the trophic condition, resulting in the local variations. To test this hypothesis, two estuaries with different organic content were compared; Kodono River (Kochi Prefecture, 33° N) which was relatively oligotrophic and Sakata River (Hiroshima Prefecture, 34° N) which was highly eutrophicated. The Kodono River population showed slow growth after settlement, and growth stopped in May to June. This population survived for only one or two years, resulting in small MTLe. On the other hand, the Sakata River population showed fast growth from August to December. This population survived for three years, resulting in large MTLe. Maturation was estimated to occur two years after settlement in both populations. Low salinities may partially affect MTLe. Habitats in the Seto-Inland Sea (33.5–34.8° N) were eutrophicated, but many habitats in the subtropical islands (24–31° N) were relatively oligotrophic. Latitudinal variation of MTLe was reflected by the geographical difference in trophic condition. A negative relationship between population density and growth was found, which was explained in terms of reduced survival of recruits in the eutrophicated habitats.     

钦州湾潮流深槽的成因与稳定性探讨

据钦州湾2007年1—2月的实测潮流,结合径流、潮汐等资料,经水动力和港湾地形综合分析揭示:钦州湾的潮流深槽是潮流作用于葫芦形复式港湾的必然产物,是在内湾纳潮蓄能、湾颈狭道强流、颈口岛礁分流喷射冲刷而成的。通过对潮流流路和强度的分析,对潮流深槽的稳定性进行了探讨;进而通过湾颈流速-内湾纳潮关系计算,给出了潮流深槽稳定的临界条件。     

非等间距GM(1,1)模型的总体最小二乘算法及其病态问题

在非等间距GM(1,1)模型中,系数矩阵中有无误差的常数项和有误差的随机项,并且系数矩阵与观测向量误差同源,即系数矩阵与观测向量中有相同的元素存在,这些相同元素应该有相同的改正数,为此本文推导了一种适合非等间距GM(1,1)模型求解的总体最小二乘算法。同时,考虑到非等间距GM(1,1)模型中存在病态问题时影响总体最小二乘计算结果的稳定性,提出对系数矩阵常数列乘以某一常数的方法,以改善病态问题。     

Study on General Performance of Jack-up Under Elevated Condition

Method of checking for jack-up elevated performance including leg structure strength, fixation system or jacking system bearing capacity, pre-load requirements, spud can bearing capacity and overturning stability is suggested in this paper. As an example, a jack-up with truss legs is analyzed by finite element analysis method. This paper may be helpful to the rig owners, operators and designers.