鱿鱼(Squid)肝脏蛋白酶解物中ACE活性抑制肽的分离纯化及活性分析

为提高鱿鱼加工下脚料的利用率,以秘鲁鱿鱼肝脏为研究对象,采用生物酶解技术和凝胶蛋白分离技术等对其进行研究。研究结果表明:鱿鱼肝脏蛋白酶解液经过超滤处理后(分子质量为20k Da),用Sephadex G-100初步分离,得到五个组分,对分离的组分进行研究,结果表明降血压活性能力最强的是组分二,测定其半抑制浓度(IC50)为1.8mg/m L。对组分二进行活性条件分析,结构表明其ACE抑制活性在温度0—100°C、p H 1—12之间基本没有变化。最后用DEAE阴离子交换柱和Sephadex LH-20对组分二继续分离纯化,得到组分六和组分七,测定其半抑制浓度(IC50)分别达到1.52 mg/m L和2.16 mg/m L。本研究可为利用鱿鱼加工下脚料开发降血压活性肽产品提供理论基础。     

数据同化在海洋生态模型中的应用和研究进展

将数据同化方法引入海洋生态系统动力学模型研究,利用现有的观测数据,获得最佳的模式参数、初始场或提高状态模拟,是当前多学科交叉研究的热门领域。本文依据国内外研究进展,主要就海洋生态模型研究中所采用的变分伴随、卡尔曼滤波、模拟退火法方法进行了介绍,总结了数据同化在我国的海洋生态系统研究中的现状和发展趋势。     

徐州市水土流失时空变化研究

本文以3S技术为支撑,在修正水土流失方程（RUSLE）的基础上,针对徐州黄泛平原-丘陵地带的特殊地形地貌、对2000年以来徐州市水土流失时空变化特征进行了分析。结果表明：2000-2014年徐州市土壤侵蚀等级以微度为主,微度侵蚀面积占总侵蚀面积多年平均达到76.34%;中度侵蚀次之,所占比例在10%~17%间;剧烈侵蚀所占比例最少。轻度及以上土壤侵蚀等级主要发生在中部微山湖下游、京杭大运河一带的丘陵岗地地区、主城区与故黄河北岸的黄泛平原地区,以及丰县、新沂、邳州的局部区域;土壤侵蚀存在一定程度年际变化特征,睢宁、丰县、邳州的部分地区侵蚀面积年际变化较为明显,土壤侵蚀总面积整体上呈现下降趋势。     

Research on the Application of Fast-steering Mirror in Stellar Interferometers

In stellar interferometers, the fast-steering mirror (FSM) is widely utilized to correct the wavefront tilt caused by the atmospheric turbulence and internal instrumental vibration, because of its high resolution and fast response frequency. In this study, the non-coplanar error between the FSM and the actuator deflection axis introduced by the manufacturing, assembly, and adjustment is analyzed systematically. Via a numerical method, the additional optical path difference (OPD) caused by the above factors is studied, and its effect on the fringe tracking accuracy of a stellar interferometer is also discussed. On the other hand, the starlight parallelism between the beams of two arms is one of the main factors for the loss of fringe visibility. By analyzing the influence of wavefront tilt caused by the atmospheric turbulence on fringe visibility, a simple and efficient real-time correction scheme of starlight parallelism is proposed based on a single array detector. The feasibility of this scheme is demonstrated by a laboratory experiment. The results show that after the correction of fast-steering mirror, the starlight parallelism meets preliminarily the requirement of a stellar interferometer on the wavefront tilt.     

马氏珠母贝种苗规模化繁育新技术及工艺

对马氏珠母贝人工育苗换水、投附着基和饵料等关键环节进行了研究。结果表明:(1)不换水组的D形幼虫及壳顶幼虫的存活率,稚贝育成率以及D形幼虫、壳顶幼虫及稚贝壳长日生长率比换水组分别提高了15.3%、259.6%、186.5%、33.3%、34.2%、12.4%,且差异显著;(2)第1、2次投附着板组的稚贝壳长日生长率均比一次性投附着板组快,第3次投附着板组的壳长日生长率比其他所有组均慢,且差异均显著。多次投附着板组的同一批次稚贝均匀度均比一次性投附着板组好,且多次投附着板组比一次性投附着板组的稚贝育成率提高了32.5%,稚贝存活率提高了19.3%,采苗量提高了35%;(3)投喂虾塘水组稚贝存活率、育成率及壳长日生长率比投喂50%自溶酵母+50%小球藻组分别提高了28.1%、47.2%、35.9%,而投喂这两种不同饵料的稚贝阴干后的存活率差异不显著。研究表明,通过封闭不换水育苗、多次投附着板及投喂虾塘水中的生态饵料的方法可以高效地培育出健康的马氏珠母贝种苗。     

海绵内生真菌胞外多糖理化性质及自由基清除活性研究

从1株海绵内生真菌(Alternaria sp.)链格孢菌的发酵液中提取胞外多糖,对其进行Q Sepharose FF离子交换色谱分离纯化,得到2个组分JJY-W和JJY-S。运用各种化学方法及波谱方法对JJY-W和JJY-S的理化性质及结构进行分析,并对多糖清除自由基活性进行了评价。结果表明,JJY-W和JJY-S的分子量分别为1.4KD和1.8KD;JJY-W以半乳糖、葡萄糖为主,含有少量甘露糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=1∶2.5∶11。JJY-S以甘露糖、葡萄糖为主,含有少量半乳糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=3∶2∶1。JJY-W总糖含量为46.3%,未检测出糖醛酸,蛋白含量为2%。JJY-S总糖含量为52%,糖醛酸含量为6.1%,蛋白含量为14%。活性分析表明,2种多糖均具有一定的体外抗氧化活性,并随着浓度的增加清除自由基能力均增强,JJY-W清除DPPH自由基的活性要强于JJY-S,而JJY-S清除OH.自由基的活性要强于JJY-W。     

Ring foundation on elastic subgrade: an analytical solution for computer modelling using the Lagrangian multiplier method

In the practice of geotechnical engineering, the case of a ring footing carrying a set of concentrated point loads is a common problem. At times, the induced vertical and angular displacements for the ring footing need to be evaluated at a relatively precise level. By making use of the governing set of equations derived for the case of a general curved beam, expressions that can be easily implemented in modern computing software are derived for the vertical and angular displacements of a ring footing of rectangular cross section as functions of the radial position. The loading case considered is a vertical point load, and the soil is modelled as elastic. Estimates of the displacements have been shown for a common range of practical applications. The behaviour for a set of concentrated loads may be evaluated using the derived equations through direct superposition. Nonlinear finite element analysis is used to evaluate the vertical deflection and angular twist of the ring foundation. Numerical analysis performed for three ring foundations with different radii and cross sections is reported to validate the accuracy of the derived analytical solution. Copyright © 2016 John Wiley & Sons, Ltd.     

离心式泥浆机械净化设备的研制与应用

本文介绍了两种离心式泥浆净化设备——NL-227型离心除泥器和WL-230型连续除泥离心机的结构、特点和应用效果,讨论了几种净化设备的分离物的粒度分布.     

Distribution and migration of pesticide residues in mosquito control impoundments St. Lucie County, Florida, USA

This project was designed to: (1) document the distribution and migration of organochlorine pesticide residues within marsh substrates of 18 St. Lucie County mosquito control impoundments located along the Indian River Lagoon estuary, and (2) evaluate the impact of water management techniques on residue mobility. Our results indicate that detectible concentrations of organochlorine compounds, applied between the late 1940s and early 1950s, are present in 16 of the 18 St. Lucie County mosquito control impoundments. These compounds are primarily restricted to the surficial, organic-rich wetland sediment, which, based upon geotechnical analysis, was exposed to the atmosphere at a time when the impoundments were subjected to pesticide treatment. Contaminated sediments are present below the surficial, organic-rich layer, suggesting that some vertical migration of pesticides has occurred. It is unlikely that leaching associated with the downward percolation of impounded water was responsible for this migration as pesticide residues were never detected within thein situ pore waters. An alternative explanation is that biological processes (e.g., rooting, burrowing) facilitated the downward flux of organochlorine compounds into sediment horizons not subjected to direct treatment. Eighty-eight surface water samples obtained from two impoundments subjected to contrasting water management techniques were analyzed for pesticide content. None of the surficial water samples collected in association with these impoundments contained detectible concentrations of organochlorine compounds. These samples were unfiltered and contained as much as 25 mg/1 of particulate organic matter. This suggests that the currently preferred management technique (RIM), which is designed to maintain water quality, limit mosquito production, and provide for ecological continuity, does not hydraulically mobilize pesticide residues into the Indian River Lagoon estuary.     

利用SPACE LAB彩色红外影像及数据复合技术对藏北牧草资源进行计算机分类的研究

本文就在藏北草资源调查中,应用空间实验室量测摄影机(MC)摄得的彩色红外影像,及根据遥感影像目视判读所利用的信息空间特点和草场分类原理进行计算机自动分类的理论与方法,对利用辅助数据和影像数据复合技术的几种尝试进行了讨论,并依实地调查资料和专家判读结果对各种分类结果进行了评价。说明利用这种航天遥感资料,辅以Landsat影像生物量指标、DTM和坡度、坡向数据以及计算机自动分类技术,进行资源调查是可行的,可以达到最佳效果。