Relevance of intracellular partitioning of metals in prey to differential metal bioaccumulation among populations of mummichogs (Fundulus heteroclitus)

Intracellular partitioning of trace metals is critical to metal tolerance in aquatic organisms and may also influence metal trophic transfer in ecosystems. In this study, we tested the relevance of metal (Cd, Cu, Pb, and Zn) intracellular partitioning in prey as an indicator of metal trophic availability to benthic forage fish, mummichogs (Fundulus heteroclitus), in chronically metal-polluted salt marshes in New York, USA. Two common prey of mummichogs in the study area, Palaemonetes pugio and Nereis acuminata, generally stored increasingly higher proportions of non-essential metals (particularly Pb) in insoluble (less trophically available) cellular components, as the whole body burdens increased. In contrast, intracellular partitioning of essential metals (Cu and Zn) in invertebrate prey varied relatively little among sites. Differential Cd and Pb intracellular partitioning patterns within P. pugio among sites were significantly associated with Cd and Pb whole body burdens in mummichogs, respectively (i.e., prey-driven bioreduction of metals), while bioaccumulation of Cu and Zn in mummichogs was similar among populations. The findings in this study suggest that metal intracellular partitioning within prey may be partially responsible for metal trophic availability to a predator in metal-polluted habitats, while there was also evidence that some predator-dependent processes may offset differential trophic availabilities from prey.     

基于ArcGIS和MapObjects的公交查询系统

公交车搭乘是武汉市市民日常出行的主要手段之一。合理的公交搭乘方案能够减少市民出行在时间和精力方面的损耗,本文以武汉市道路网为基础,阐述了通过ArcGIS进行公交网络数据模型构建的过程,并利用VisualC＋＋和Map Objects实现了公交简易查询系统的开发。     

基于等角比例投影的球面三角四叉树剖分模型

将人不可及的观测统称为遥感,使地面传感器成为遥感的一部分.提出遥感科学与技术的基本内容,包括传感器研制、遥感数据获取、数据处理、信息提取和应用5个部分.其理论基础是辐射传输过程模拟与反演,贯穿于各个组成部分.数据的可视化及传输发布存在于从数据获取到应用的各个环节.遥感科学与技术正处于快速发展时期,各个方面都有前沿问题.辐射传输理论中的尺度和多角度问题,地形对辐射和图像几何影响及相应的多源多时相数据的匹配问题,遥感信息提取中自动化和目视解译的结合以及信息提取新策略问题,遥感与基于过程模拟的气候一水文-生态模式相结合的四维数据同化技术等是当前遥感科学与技术发展中的重要学科前沿.四维数据同化技术是突破遥感瞬时信息获取、实现图像与机理模型有机结合、改善自身数据产品质量和促进地学大范围模拟准确性的重要发展方向,能增强遥感对全球变化科学的贡献.     

Parameter estimation for basin-scale ecosystem-linked population models of large pelagic predators: Application to skipjack tuna

A Spatial Ecosystem and Population Dynamic Model (SEAPODYM) is used in a data assimilation study aiming to estimate model parameters that describe dynamics of Pacific skipjack tuna population on ocean-based scale. The model based on advection–diffusion–reaction equations explicitly predicts spatial dynamics of large pelagic predators, while taking into account data on several mid-trophic level components, oceanic primary productivity and physical environment. In order to improve its quantitative ability, the model was parameterized through assimilation with commercial fisheries data, and optimization was carried out using maximum likelihood estimation approach. To address the optimization task we implemented an adjoint technique to obtain an exact, analytical evaluation of the likelihood gradient. We conducted a series of computer experiments in order to (i) determine model sensitivity with respect to variable parameters and, hence, investigate their observability; (ii) estimate observable parameters and their errors; and (iii) justify the reliability of the computed solution. Parameters describing recruitment, movement, habitat preferences, natural and fishing mortality of skipjack population were analysed and estimated. Results of the study suggest that SEAPODYM with achieved parameterization scheme can help to investigate the impact of fishing under various management scenarios, and also conduct forecasts of a given species stock and spatial dynamics in a context of environmental and climate changes.     

Word2003文档中插入AutoCAD2004图形的三种方法

对于测绘技术人员来说,需要编写设计书、技术总结、监测方案等测绘类文档。利用Word2003自带的绘图工具,远远不能满足要求。介绍了“利用复制＋粘贴工具”、“转换图像格式”、“使用插入对象”三种方式在Word2003文档中插入AutoCAD2004图形的三种方法,     

基于FME语义映射的数据转换及应用

在详细介绍FME语义映射和转换规则的基础上,分析了FME的空间数据转换机制和简单语义转换规则的不足,并基于自定义映射文件提出了一种在DOS环境下进行批量数据转换的高效方法,最后以DGN到DWG格式的数据转换实例探讨了实施FME语义转换的具体步骤。     

Nonlinear transfer function modelling of beach morphology at Duck,North Carolina

This paper presents a simple nonlinear data-based modelling approach for predicting the beach profile volume at Duck, North Carolina, USA. The state-dependent parameter form of the general transfer function (SDP TF) model is used to describe nonlinearity influencing these morphological data in two case examples. Case 1 investigates the nonlinearity associated with the dependency of wave forcing on the preceding beach volume. Case 2 investigates the ability to model the variables within the well-known diffusion equation for beach volume using this data-based approach. The results of this study show that the SDP TF approach can be used successfully to develop statistically robust models for describing nonlinearity in beach morphological systems. Furthermore, these models are shown to predict the beach volumes over both short (1 month ahead) and long (2 years ahead) time periods, and thus show great potential for practical applications in coastal zone management and engineering.     

无线VPN技术在数字前兆台网中的应用

VPN技术提供了一种通过公用网络安全地对企业内部专用网络进行远程访问的连接方式。介绍了VPN的技术和原理,并以湖北省数字前兆台网为例,提出了一种无线VPN技术的连接设置方式。它具有运行成本低、使用方便、无线发送、有线接入等特点。     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.