脊尾白虾(Exopalaemon carinicauda)维甲酸X受体基因克隆及其在温盐胁迫和蜕皮周期中的表达分析

为了明确脊尾白虾维甲酸X受体基因在环境胁迫和蜕皮周期中的作用,本研究通过RACE技术克隆了脊尾白虾(Exopalaemon carinicauda)维甲酸X受体c DNA全长,命名为Ec RXR基因,该c DNA序列全长为1323 bp,开放阅读框(ORF)为855 bp,编码一个284个氨基酸组成的蛋白质,分子量为30.918 k Da,理论等电点为PI为6.788;Ec RXR基因推导氨基酸序列经Blastp在线比对分析显示,Ec RXR与已知甲壳动物RXR的同源性为71%—90%;系统进化树分析结果显示,脊尾白虾Ec RXR的氨基酸序列与日本沼虾RXR的氨基酸序列聚为一支。荧光定量RT-PCR分析表明,Ec RXR基因在各组织中均有表达,而在眼柄相对表达量较高,血淋巴中最低。Ec RXR基因在蜕皮周期中的表达规律表明,Ec RXR基因表达在不同蜕皮时期存在明显变化,在眼柄、肝胰腺、胃和肠中整体呈现上升趋势,在鳃中呈现先下降后上升的趋势,在表皮中呈现逐渐降低的趋势。Ec RXR基因在温度、盐度胁迫过程中的表达规律分析结果发现,温度、盐度胁迫均可显著改变Ec RXR基因在鳃和肝胰腺中的表达模式,温度胁迫下Ec RXR基因在鳃中呈先上升后下降的表达趋势,肝胰腺中整体呈先上升后下降再上升再下降的表达趋势;盐度对鳃和肝胰腺的调控模式相同,均呈先升高后下降的变化趋势。本研究结果表明Ec RXR基因在脊尾白虾蜕皮发育、酶活性调控及渗透压调节中发挥重要作用,为深入研究甲壳动物维甲酸X受体基因的功能提供了重要的基础信息。     

温度、饵料种类对中华哲水蚤(Calanus sinicus)油脂积累与生长发育的影响

中华哲水蚤(Calanus sinicus)C5期幼体的油脂积累是种群在黄海冷水团中得以顺利度夏的关键过程。本研究对温度(10°C和19°C恒温,10—19°CⅠ和10—19°CⅡ变温)与饵料种类(硅藻饵料,自然饵料)双因子培养实验进行研究,探讨温度和饵料种类对中华哲水蚤油脂积累与生长发育的影响作用。结果表明,不同的温度和饵料种类对C5期幼体的油脂积累均有影响。C5期幼体在变温组的油脂积累是10°C组的31%—102%,是19°C组的1.8—6.1倍,低温有利于C5期幼体降低个体代谢消耗以增加油脂的积累。在恒温培养下,C5期幼体在硅藻饵料组的油脂积累是自然饵料组的2.8倍,硅藻饵料比自然饵料更有利于油脂的积累。雌体的体长和油囊体积均随着温度的升高而减小。与硅藻饵料相比,在自然饵料组中雌体的性腺发育速度更快,性腺成熟度更高(繁殖指数:58%—65%)。     

基于长期观测的辽东湾口东部海域水动力特征研究

辽东湾口东部海域是辽东湾与渤海中部进行物质和能量交换的主要通道之一。本文利用坐底式海床基平台获取的近8个月的水动力连续观测资料,通过谱分析和调和分析方法对该海域的潮汐、潮流特征进行分析,并讨论了余流及底层温度的季节变化规律。研究结果表明:该海域潮汐属于不规则半日潮,平均潮差为0.95 m,最大可能潮差为2.27 m。潮流属于不规则半日潮流,M₂分潮流为其优势分潮流。主要分潮流运动形式为往复流,最大流速方向为西南-东北向。余流的季节性特征较为明显:秋季,余流流速在中层达到最大,流向以西南向为主;冬季,余流流速垂向变化较小,并呈西南偏西向流动;春季,流速随深度增加而减小,流向从表层至底层呈现逆时针旋转的特征。受底层潮流、水平温度梯度及海面温度日变化的影响,底层温度表现出短期的高频变化特征:秋季,短期振荡以半日周期信号为主;冬季,全日周期信号较为显著;春季,短期振荡的现象较弱。     

海洋上层暖水在热带气旋迅速增强中的作用:基于台风威马逊(1409)的个例研究

在登陆海南岛之前,台风威马逊在南海北部从热带风暴级别迅速增强成为超强台风。观测数据的分析结果显示,海洋上层的异常暖水在威马逊的迅速增强过程中扮演了重要的角色。威马逊期间,南海北部的海表面温度相比于气候态海表面温度暖很多。这部分异常暖水为威马逊提供了更多的能量,从而导致了威马逊的迅速增强。数值模拟结果进一步证明,南海北部的暖水在台风威马逊的迅速增强过程中起重要作用。如果没有这团异常暖水的影响,威马逊只增强25 hPa,仅为有暖水影响条件下增强程度的58.1%。     

基于MTSAT卫星遥感的中国海及西北太平洋海域海表温度日增温研究

Hourly sea surface temperature(SST) observations from the geostationary satellite are increasingly used in studies of the diurnal warming of the surface oceans. The aim of this study is to derive the spatial and temporal distribution of diurnal warming in the China seas and northwestern Pacific Ocean from Multi-functional Transport Satellite(MTSAT) SST. The MTSAT SST is validated against drifting buoy measurements firstly. It shows mean biases is about –0.2°C and standard deviation is about 0.6°C comparable to other satellite SST accuracy. The results show that the tropics, mid-latitudes controlled by subtropical high and marginal seas are frequently affected by large diurnal warming. The Kuroshio and its extension regions are smaller compared with the surrounding regions. A clear seasonal signal, peaking at spring and summer can be seen from the long time series of diurnal warming in the domain in average. It may due to large insolation and low wind speed in spring and summer, while the winter being the opposite. Surface wind speed modulates the amplitude of the diurnal cycle by influencing the surface heat flux and by determining the momentum flux. For the shallow marginal seas, such as the East China Sea, turbidity would be another important factor promoting diurnal warming. It suggests the need for the diurnal variation to be considered in SST measurement, air-sea flux estimation and multiple sensors SST blending.     

温度、盐度和光照对浒苔生长和光合作用的影响

Intensive Pyropia aquaculture in the coast of southwestern Yellow Sea and its subsequent waste, including disposed Ulva prolifera, was speculated to be one of the major sources for the large-scale green tide proceeding in the Yellow Sea since 2007. It was, however, unclear how the detached U. prolifera responded and resumed growing after they detached from its original habitat. In this study, we investigated the growth and photosynthetic response of the detached U. prolifera to various temperature, salinity and irradiance in the laboratory. The photosynthetic rate of the detached U. prolifera was significantly higher at moderate temperature levels(14–27°C)and high salinity(26–32), with optimum at 23°C and 32. Both low(14°C) and highest temperature(40°C), as well as low salinity(8) had adverse effects on the photosynthesis. Compared with the other Ulva species, U. prolifera showed higher saturated irradiance and no significant photoinhibition at high irradiance, indicating the great tolerance of U. prolifera to the high irradiance. The dense branch and complex structure of floating mats could help protect the thalli and reduce photoinhibition in field. Furthermore, temperature exerted a stronger influence on the growth rate of the detached U. prolifera compared to salinity. Overall, the high growth rate of this detached U. prolifera(10.6%–16.7% d~(–1)) at a wide range of temperature(5–32°C) and salinity(14–32) implied its blooming tendency with fluctuated salinity and temperature during floating. The environmental parameters in the southwestern Yellow Sea at the beginning of green tide were coincident with the optimal conditions for the detached U. prolifera.     

大连蜈蚣藻孢子发育及生活史的研究

在实验室条件下,对大连蜈蚣藻(Grateloupia dalianensis H.W.Wang et D.Zhao)的孢子早期发育、盘状体的形成和直立枝生长进行了详细研究,对其生活史进行了详细观察,并进行了温度和光照强度对孢子发育影响的研究。结果表明:(1)孢子发育类型为间接盘状体型;(2)生活史由雌、雄配子体、四分孢子体和果孢子体三相世代组成,配子体与孢子体形态相同,属于同型世代交替,与属模蜈蚣藻(G.filicina)一致;(3)温度对盘状体和直立枝生长均有影响,最适温度均为16℃;(4)光照强度对盘状体和直立枝生长均有影响,最适光照强度分别为7 500 lx和10 000 lx。     

国产抛弃式温度剖面仪(XBT)资料质量分析

国家海洋技术中心根据科研需求研发了抛弃式温度剖面仪(XBT)等一系列抛弃式产品.针对2014年8月份在西太平洋海域投放的XBT和XCTD所获取的温度数据进行对比分析,单剖面结果显示两者相关系数达到了0.95,在温跃层处出现温度差,断面标准差分析结果为200 m以上温跃层处较大而200m以下标准差较小,最大和最小标准差值分别为0.39和0.08.温度断面分析结果显示两者在同样的位置出现等值线的凹凸现象,对大洋水团特殊物理海洋现象描述基本一致.分析温跃层处两者温度存在偏差的原因有3个:不同传感器的不同响应时间引起的误差、深度测量公式的误差以及传感器本身的测量误差影响.国产XBT的数据质量较好,总体上性能能满足对环境复杂海域的快速走航观测,数据质量准确度和可靠性都较好.     

Electrical analogy for modelling thermal regime and moisture distribution in sandy soils

The soil mass is subjected to temperature variation due to several human activities (viz. tanks storing heated fluids, buried cables and pipelines, air-conditioning ducts, disposal of nuclear and thermal power plant wastes etc.), which result in heat-induced migration of the moisture in it. Though several studies have been conducted in the past to investigate the mechanism of heat migration through the soil mass, a methodology for ‘real-time measurement of the variations in temperature, flux and moving moisture front, in tandem, with respect to space' has rarely been attempted. In this context, extensive laboratory investigations were conducted to measure real-time flux and temperature variations in the sandy soils, and the validation of results has been done by employing an equivalent electrical circuit programme, LTspice. Subsequently, a mathematical model PHITMDS (i.e. Prediction of Heat-Induced Temperature and Moisture Distribution in Soil) has been developed and its utility and efficacy, for predicting the depth-wise temperature and heat-induced moisture migration, due to evaporation, in terms of position of moving moisture front in the sandy soil has been critically discussed and demonstrated.     

渝东南地区下寒武统牛蹄塘组页岩孔径分布测试方法研究

低温氮气吸附法已普遍用于评价页岩的孔隙结构,但是文献中报道的脱气温度和脱气时间等测试条件不尽相同,此外测试结果中经常出现滞后环不闭合的情况,严重影响了孔径分布等结构参数测试的准确性。为了评价和探索有效的页岩孔径分布测试方法,本文选取渝科1井下寒武统牛蹄塘组黑色页岩,重点研究应用低温氮气吸附法分析其孔径分布的测试条件,系统探讨了样品质量、脱气温度、升温速率和脱气时间对测试结果的影响,通过进一步优化参数建立了可靠的孔径分布测试方法,并成功用于测定其他页岩样品。结果表明:吸附-脱附等温线呈反C型,属于Brunauer等提出的BDDT等温吸附曲线分类中的V型,滞后环完全闭合,属于IUPAC分类中的H4型,对应狭窄的狭缝型孔隙,说明此类页岩中除了含有峰值孔径主要集中在3.5~4.5 nm的中孔和一定数量的大孔外,还存在大量微孔。研究认为脱气温度是影响测试结果的主要因素。该研究是页岩孔径分布测试方法的一项补充,为我国页岩气的深入研究提供了关键参数支持。