麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

虚拟样机技术在地质工程机械领域的应用研究

基于对国内地质工程机械的研发现状的考虑，根据虚拟样机技术的特点，讨论了在地质工程机械领域实施虚拟样机技术的必要件，并提出了建立地质工程机械虚拟样机的策略。联合PRO／E、ANSYS及ADAMS建立了气动潜孔锤的虚拟样机模型，对气动潜孔锤虚拟样机进行了仿真，阐明了虚拟样机技术在地质工程机械领域的应用前景。     

Weather as a Chronic Hazard for Road Transportation in Canadian Cities

Inclement weather creates a chronic hazard for Canadian travellers. Past studies indicate that road collision rates increase during precipitation, although the magnitude of theincrease varies from study to study, partly as a result of variations in weather and driving conditions,but also because of differences in methods. The goal of the current study is to improve ourunderstanding of the links between weather and travel risk in mid-sized Canadian cities by using astandardized method for analyzing data from six cities with different climates: Halifax-Dartmouth,Ottawa, Québec, Hamilton, Waterloo Region, and Regina. The study has four interrelated objectives: (1) Toconduct a sensitivity analysis to determine the extent to which risk estimates vary depending onthe criteria used to define precipitation events and `normal' conditions; (2) To compare therelative risk of collision and injury during precipitation relative to `normal' conditions; (3) Todetermine the extent to which weather-related risks vary fordifferent Canadian cities; and (4) To explore any differences in collision characteristics between events and controls, especially as theyvary from city to city. Results are based on a matched-pair analysis, using six-hour time blocks over afour-year period, 1995 to 1998. Results indicate only modest sensitivity to the criteria used to define precipitation events and `normal' conditions. On average, precipitation is associated with a 75 percentincrease in traffic collisions and a 45 percent increase in related injuries, as compared to `normal'seasonal conditions, but risk levels vary depending on the characteristics of the weather event.Both sensitivity to specific weather conditions and weather-related accident profiles vary from city tocity in ways that are not easily explained.     

旋挖钻进中硬岩及卵砾层钻头结构及施工工艺研究

旋挖钻进技术作为一种高效优质的钻进方法近年来广泛地被用于公路、铁路及大型水电站基础工程施工建设中，取得了良好的社会效益和经济效益。探讨了在卵砾石、漂石等复杂地层以及坚硬基岩中进行旋挖钻进所用钻头的结构及其施工工艺。     

螺杆钻具转子表面磨损规律研究

转子是螺杆钻具的核心部件,与定子相配合,决定了螺杆钻具的工作效率和动力性能。主要研究不同泥浆固相含量和添加剂对螺杆钻具转子表面磨损的影响,并设计了专用试验装置以模拟近井下条件工作时螺杆钻具转子的表面磨损情况,对其磨损规律开展研究,探索了转子在不同泥浆条件下的磨损规律,并结合相关磨损机理进行了进一步分析。初步发现了对硬铬镀层损伤较大的因素是泥浆固相与盐类添加剂,进而确定了硬铬镀层磨损的主要形式是腐蚀磨损,从而形成了对转子的制造工艺及使用方法的有益建议。     

螺杆钻定向钻探技术研究与应用

论述了螺杆钻定向钻探配套器具的研究、施工工艺及应用典型实例，提出了今后液项技术的发展方向。     

Field Evaluation of the Vertical Bearing Capacity of a Screw Pretensioned Spun High-Strength Concrete Pile

This study has evaluated the vertical bearing capacity by conducting static load tests for noise-free and vibration-free screw pretensioned spun high-strength concrete (PHC) piles installed using two different methods (end-squirting shoe and pre-boring methods). Vertical bearing capacity differences seem to occur due to the displacement of soils near the external circumference of a pile, depending on the installation method. A method by which to evaluate the bearing capacity of screw concrete piles is suggested by considering the equations that already have been used to calculate the bearing capacity of piles. Based on static load tests and analysis, the pile installed using the end-squirting shoe method was assumed to be a bored pile and it was reasonable to use the equation proposed by the Japanese Geotechnical Society. At the same time, the pile installed using the pre-boring method was deemed a low soil displacement pile and so it was reasonable to apply the equations proposed for calculating the bearing capacity of the driven pile suggested by the Architectural Institute of Japan.     

Development of Prebored Screw Pile Method and Evaluation of Its Bearing Characteristics

The pile-driving method produces considerable noise and vibrations. Hence, an auger-drilled pile method was developed as a low-noise and -vibration substitute. However, this method does not guarantee the bearing capacity of the pile unless some amount of pile-driving is performed. Therefore, the noise and vibration problems cannot be completely solved. In this study, a prebored screw pile method is proposed to solve these problems. In this method, piles are constructed by the rotary penetration of a screw pile into a prebored hole filled with some cement milk and whose diameter is smaller than that of the screw pile. To determine the shape of the screw pile, laboratory tests with model screw piles were conducted. Also, field load tests were conducted on an actual screw pile fabricated based on the laboratory test result and on a smooth-surfaced pile. In addition, the behavior of the screw pile was estimated by using three-dimensional finite element analysis. The results of the field load test and the numerical simulation showed that the ultimate bearing capacity and the unit skin friction of the screw pile are very superior to those of the smooth-surfaced pile and the cement milk is an important factor in the prebored screw pile method.     

贵州省复杂地层地热深井钻探工艺

贵州省地矿局组织实施了“贵州省地热深井钻探技术攻关”项目,研究了喀斯特地区复杂地层情况下地热深井钻探中螺杆钻井工艺和空气钻井工艺的适应性,攻克了两种工艺在该地区应用受地层特性限制的相关技术难题。螺杆马达平均寿命达到200小时以上,螺杆钻井工艺较常规钻井工艺钻进机械钻速提高2～3倍;空气潜孔锤单井应用深度超过1500m,空气潜孔锤钻井工艺较常规钻井工艺机械钻速提高10～29.5倍。同时研究了单井多工艺转换接力钻进技术,以及与上述工艺配套使用的钻井液体系。最终形成了一套适合贵州省地质条件地热深井钻探施工并能显著提高钻井效率和经济效益的组合工艺技术体系。该成果能将1口2000m以上地热深井施工周期由原来的8~12个月缩短至4~6个月,将单井平均施工成本由450万元降低至410万元以下。     

微波等离子体炬原子发射光谱法测定铁和铝的研究

研究了低功率微波等离子体炬原子发射光谱法（ＭＰＴ－ＡＥＳ）对Ｆｅ，Ａｌ的测定。样品经气动雾化后，分别以流动注射与连续式两种方式引入ＭＰＴ。考察了用这两种进样方式时ＭＰＴ－ＡＥＳ的分析性能，证明低功率ＭＰＴ－ＡＥＳ具有良好的分析特性。