河北省近海有害藻华灾害分级、时空分布和优势藻华生物变化特征

为研究1989—2013年河北省近海有害藻华灾害分级、时空分布和优势肇事生物变化特征,对河北省近海累计58次有害藻华记录的发生时间、位置与范围、面积和优势有害藻华生物种类进行了统计分析。结果表明:小型(面积0~100 km2)、中型(100~1 000 km2)、大型(≥1 000 km2)和面积不详的有害藻华中,小型藻华为多发性藻华,发生次数占全省海域累计发生次数的60.35%;5-8月份是藻华多发期,发生次数占累计发生次数的86.21%;2000年后藻华发生频率大增,由原来年均发生1次演变为年均发生4次;秦皇岛海域发生藻华次数最多,占全省海域累计发生次数的57.14%,沧州海域次之,唐山海域最少;秦皇岛海域发生藻华累计面积最大,占全省海域累计发生面积的64.25%,沧州海域次之,唐山海域最小。河北省优势海洋有害藻华生物共计15种,易发性藻华生物夜光藻Noctiluca scintillans引发藻华次数最多,占全省海域累计发生次数的44.83%,2009年前是河北省近海最主要的藻华肇事生物;高发性藻华生物抑食金球藻Aureococcus anophagefferens引发有害藻华累计面积最大,占全省海域累计发生面积的55.57%,2009年后该藻和夜光藻共同成为河北省近海优势藻华肇事生物。     

Oxygen consumption in the epilimnia and hypolimnia of two eutrophic,warm‐monomictic lakes

Oxygen consumption was estimated for two eutrophic New Zealand lakes using a simple 2‐layer model, from estimates of net changes in oxygen, phytoplankton photosynthesis, inflow and outflow, diffusion, eddy diffusion, and changes in the depth of the thermocline. Of the total oxygen consumption, 75–85% occurred in the epilimnia. Epilimetic oxygen consumption per m³ and per m² in Lake Johnson were higher than in Lake Hayes, reflecting known differences in the trophic status of the lakes, but estimates for the hypolimnia of the two lakes were similar. Daily hypolimnetic areal deficits were sensitive to both the depths and time intervals chosen.

In the epilimnia, monthly oxygen production by photosynthesis averaged 1.1 and 1.8 times the initial oxygen content in Lakes Hayes and Johnson respectively. Diffusion appeared to be of similar magnitude to photosynthesis in the isothermal period, but was less important during stratification. Losses to the hypolimnia by eddy diffusion were less than 10% of photosynthesis. Photosynthesis in the hypolimnia contributed 30–40% of the oxygen consumed there and eddy diffusion supplied a further 20%.

In Lake Johnson a 3–5 m stratum of water in the metalimnion became anoxic for 2–3 months each summer while there were still up to 5.2 g.rer³ of oxygen remaining in the hypolimnion. Metalimnetic anoxia is attributed largely to oxygen consumption in situ, resulting from the highly eutrophic state of the lake and intense thermal stratification. Zooplankton respiration accounted for less than 10% of the maximum net decline in metalimnetic oxygen in 1970–71, but was more important in the other 2 years.

Changes in the oxygen content of the hypolimnion indicate that Lake Hayes has become more eutrophic since 1954–55. In Lake Johnson low oxygen concentrations at the autumn overturn (<5 g.m^‐3) represent a threat to the trout population.     

Nitrogen and phosphorus in New Zealand streams and rivers: Control and impact of eutrophication and the influence of land management

Abstract

Given sufficient light and heat, the growth of aquatic macrophytes and algae associated with eutrophication is generally controlled by the concentration, form and ratio between nitrogen (N) and phosphorus (P). Data from 1100 freshwater sites monitored for the last 10 years by New Zealand's regional councils and unitary authorities were assessed for streams and rivers with mean nitrate/ nitrite‐N (NNN), dissolved reactive P (DRP), total N (TN) and total P (TP) concentrations in excess of New Zealand guidelines, and to generate a data set of N:P ratios to predict potential periphyton response according to the concentration of the limiting nutrient. The frequency of sites exceeding the guidelines varied from 0 to 100% depending on the parameter and region, but South Island regions were generally more compliant. The dissolved inorganic N (DIN) to dissolved reactive P (DRP) ratio was used to group data into three nutrient limitation classes: <7:1 (N‐limited), between 7:1 and 15:1 (co‐limited), and>15:l (P‐limited), by mass. P‐limitation was the most frequent scenario in New Zealand streams (overall, 76% of sites were P‐limited, 12% N‐limited, and 12% co‐limited). The mean concentration of the limiting nutrient for each site was combined with empirical relationships to predict periphyton densities (the average of N‐and P‐limited growth was used for sites with co‐limitation). This assessment predicted that 22 sites were likely to exceed the periphyton guideline for protecting benthic biodiversity (50 mg chlorophyll a m^?2), but this assessment is likely to be highly changeable in response to climatic conditions and present and future land use. As an example, we modelled N and P losses from an average sheep and a dairy farm in Southland (South Island, New Zealand) in 1958, 1988, 2008 and 2028. We predicted that with time, as farm systems have and continue to intensify, N losses increase at a greater rate than P losses. Since the pathway for N to reach fresh waters may be more tortuous and take longer than P to reach a stream or river, focusing mitigation on P losses may have a quicker effect on potential algal growth. In addition, with time, it is expected that P‐limitation in New Zealand's rivers and streams will be more widespread as N‐losses are unabated. Hence, although strategies to decrease N losses should be practised, mitigating P losses is also central to preventing eutrophication.     

南太湖入湖口蓝藻生物量与CODMn和DO的相关性研究

采用水质监测方法对2008—2010年南太湖水域三个入湖口(小梅港、新塘港、大钱港)水体中蓝藻生物量、CODMn和DO的时空变化特征进行了监测和调查,并通过SPSS10.0软件对蓝藻生物量与CODMn和DO的相关性进行了分析。结果表明:(1)3年内南太湖入湖口蓝藻颗粒数在1.0×106个/L以上范围的频率每年超过50%,最高在3.0×107个/L左右;(2)南太湖入湖口CODMn指标大部分处于较高的污染水平,最高有6.5mg/L,最低约3.0mg/L;(3)蓝藻生物量与CODMn的相关性系数r介于0.3—0.7之间,呈现中低度正相关;(4)3年内蓝藻生物量与DO的相关性系数r介于0.30—0.50,呈现低度正相关,DO浓度有70%以上的频率大于6.0mg/L。结果表明,3个入湖口溶解氧指标能达Ⅱ—Ⅲ类地表水水质标准,但有机物污染已经不容忽视,并且与蓝藻暴发呈一定的相关性。     

我国沿海马尾藻属(Sargassum)的物种多样性及其区系分布特征

通过对我国沿海多次野外实地考查以及文献报道,采用统计学的分析方法,研究了马尾藻属海藻资源的物种构成和区系分布特征.结果表明,马尾藻属种数在我国海域的水平分布呈现出北少南多的趋势,黄、东海仅17种,南海达到124种、占世界马尾藻总数的36.47％.其中,黄海西区、东海西区、南海北区和南海南区种数及小区单一分布种类数分别为10、13、82和61种及2、3、52和42种.我国特有种类为64种、占世界尾藻总数的18.82％.在我国沿海马尾藻的垂直分布特征为:高潮带2种,低潮带91种,潮下带78种.     

Phylogenomic analysis of transcriptomic sequences of mitochondria and chloroplasts for marine red algae (Rhodophyta) in China

The chloroplast and mitochondrion of red algae （Phylum Rhodophyta） may have originated from different endosymbiosis. In this study, we carried out phylogenomic analysis to distinguish their evolutionary lin-eages by using red algal RNA-seq datasets of the 1 000 Plants （1KP） Project and publicly available complete genomes of mitochondria and chloroplasts of Rhodophyta. We have found that red algae were divided into three clades of orders, Florideophyceae, Bangiophyceae and Cyanidiophyceae. Taxonomy resolution for Class Florideophyceae showed that Order Gigartinales was close to Order Halymeniales, while Order Graci-lariales was in a clade of Order Ceramials. We confirmed Prionitis divaricata （Family Halymeniaceae） was closely related to the clade of Order Gracilariales, rather than to genus Grateloupia of Order Halymeniales as reported before. Furthermore, we found both mitochondrial and chloroplastic genes in Rhodophyta under negative selection （Ka/Ks〈1）, suggesting that red algae, as one primitive group of eukaryotic algae, might share joint evolutionary history with these two organelles for a long time, although we identified some dif-ferences in their phylogenetic trees. Our analysis provided the basic phylogenetic relationships of red algae, and demonstrated their potential ability to study endosymbiotic events.     

四种绿藻和四种褐藻脂肪酸组成的比较研究

对绿藻门和褐藻门8种大型海藻的脂肪酸组成进行了研究,发现两类海藻都有其特征脂肪酸或有几种特征脂肪酸组合做为其化学分类的标记。4种绿藻的主要脂肪酸是16:0、16:1ω7、18:4ω3、18:1ω7、18:2ω6、18:3ω3、18:1ω9,其中18:1ω7和18:3ω3的含量相对较高;4种褐藻中16:0、18:1ω9、18:2ω6、18:3ω3、18:4ω3、20:5ω3、20:4ω6的含量占绝对优势,十八碳和二十碳多不饱和脂肪酸是褐藻门脂肪酸的典型特征。另外,褐藻中含有较高含量的EPA,海带和裙带菜尤为明显。对2门类5属8株海藻所含脂肪酸进行聚类分析的结果显示海藻各门及种间的亲缘关系,表明利用静态条件下海藻脂肪酸的聚类分析结果,可在一定程度上判别海藻在分类上亲缘关系的远近,海藻脂肪酸组成的差异可以作为海藻分类的一个辅助技术手段。     

A Middle Miocene carbonate embankment on an active volcanic slope: Ilhéu de Baixo,Madeira Archipelago,Eastern Atlantic

Carbonate factories on insular oceanic islands in active volcanic settings are poorly explored. This case study illuminates marginal limestone deposits on a steep volcanic flank and their recurring interruption by deposits linked to volcaniclastic processes. Historically known as Ilhéu da Cal (Lime Island), Ilhéu de Baixo was separated from Porto Santo, in the Madeira Archipelago, during the course of the Quaternary. Here, extensive mines were tunnelled in the Miocene carbonate strata for the production of slaked lime. Approximately 10 000 m³ of calcarenite (−1 to 1ø) was removed by hand labour from the Blandy Brothers mine at the south end of the islet. Investigations of two stratigraphic sections at opposite ends of the mine reveal that the quarried material represents an incipient carbonate ramp developed from east to west and embanked against the flank of a volcanic island. A petrographic analysis of limestones from the mine shows that coralline red algae from crushed rhodoliths account for 51% of all identifiable bioclasts. This material was transported shoreward and deposited on the ramp between normal wave base and storm wave base at moderate depths. The mine's roof rocks are formed by Surtseyan deposits from a subsequent volcanic eruption. Volcaniclastic density flows also are a prevalent factor interrupting renewed carbonate deposition. These flows arrived downslope from the north and gradually steepened the debris apron westwards. Slope instability is further shown by a coral rudstone density flow that followed from growth of a coral reef dominated by Pocillopora madreporacea (Lamarck), partial reef collapse, and transport from a more easterly direction into a fore‐reef setting. The uppermost facies represents a soft bottom at moderate depths in a quiet, but shore‐proximal setting. Application of this study to a broader understanding of the relationship between carbonate and volcaniclastic deposition on oceanic islands emphasizes the susceptibility of carbonates to dilution and complete removal by density flows of various kinds, in contrast to the potential for preservation beneath less‐disruptive Surtseyan deposits. Copyright © 2013 John Wiley & Sons, Ltd.     

波吉卵囊藻的固定化培养及利用

以褐藻酸钙为固定化载体,探讨了胶球直径、不同接种量、CaCl2溶液浓度、褐藻酸钠浓度等条件对波吉卵囊藻(Oocystisborgei)固定化培养及对养殖水体水质的影响。结果表明:波吉卵囊藻固定化培养的最好条件是微藻接种量为1×106 mL-1,CaCl2溶液的质量浓度为30g/L,褐藻酸钠溶液的质量浓度为20g/L。在此条件下制备的固定化藻珠,波吉卵囊藻的生物量从1 83×10-2 mg/粒增加到17. 46×10-2 mg/粒,增加了近10倍,生长率相对较高,证明了它的生理活性不会因固定化而受干扰。引入固定化的波吉卵囊藻不但可以降低养殖水体中氨氮、亚硝酸氮等有害因子的浓度,还能提高水中溶解氧含量,使水体环境长时间处于良好的动态平衡状态。     

Estimation of net accumulation rate at a Patagonian glacier by ice core analyses using snow algae

Snow algae in a 45.97-m-long ice core from the Tyndall Glacier (50°59′05″S, 73°31′12″W, 1756 m a.s.l.) in the Southern Patagonian Icefield were examined for potential use in ice core dating and estimation of the net accumulation rate. The core was subjected to visual stratigraphic observation and bulk density measurements in the field, and later to analyses of snow algal biomass, water isotopes (¹⁸O, D), and major dissolved ions. The ice core contained many algal cells that belonged to two species of snow algae growing in the snow near the surface: Chloromonas sp. and an unknown green algal species. Algal biomass and major dissolved ions (Na⁺, K⁺, Mg²⁺, Ca²⁺, Cl⁻, SO₄²⁻) exhibited rapid decreases in the upper 3 m, probably owing to melt water elution and/or decomposition of algal cells. However, seasonal cycles were still found for the snow algal biomass, ¹⁸O, D-excess, and major ions, although the amplitudes of the cycles decreased with depth. Supposing that the layers with almost no snow algae were the winter layers without the melt water essential to algal growth, we estimated that the net accumulation rate at this location was 12.9 m a^− 1 from winter 1998 to winter 1999, and 5.1 m from the beginning of winter to December 1999. These estimates are similar to the values estimated from the peaks of ¹⁸O (17.8 m a^− 1 from summer 1998 to summer 1999 and 11.0 m from summer to December 1999) and those of D-excess (14.7 m a^− 1 from fall 1998 to fall 1999 and 8.6 m a^− 1 from fall to December 1999). These values are much higher than those obtained by past ice core studies in Patagonia, but are of the same order of magnitude as those predicted from various observations at ablation areas of Patagonian glaciers.