秦皇岛近海褐潮高发区浮游植物的碱性磷酸酶活性分析

碱性磷酸酶(Alkaline phosphatase,AP)是浮游植物在磷胁迫状态下表达的一种水解有机磷源的胞外酶,可用于指示海区浮游植物的磷胁迫状态。本研究于2013年7月,对秦皇岛近海抑食金球藻(Aureococcus anophagefferens)褐潮发生期间浮游植物的碱性磷酸酶活性(AP activity,APA)进行研究,结合其他理化参数,分析藻华发生时浮游植物的磷营养状态及其对海水中磷源的水解与利用情况。结果表明,褐潮发生时,抑食金球藻细胞密度高达108个/L,溶解有机磷(Dissolved organic phosphorus,DOP)成为浮游植物生长利用的主要磷源。抑食金球藻的细胞密度受到海水中NO3–、DOP、溶解无机磷(Dissolved inorganic phosphorus,DIP)浓度等的显著影响。浮游植物大量表达AP水解DOP,平均APA高达217.72 nmol/(μg·h)±90.86 nmol/(μg·h)(350.44 nmol/(L·h)±130.57 nmol/(L·h)),且APA随浮游植物生物量增大而显著增加。该结果表明抑食金球藻褐潮发生时,海区遭受严峻的磷胁迫甚至限制。磷源,尤其是有机磷源的可利用性可能在秦皇岛海区抑食金球藻褐潮的发生和维持中起关键作用。     

Effects of lakes and reservoirs on annual river nitrogen,phosphorus, and sediment export in agricultural and forested landscapes

Recently, effects of lakes and reservoirs on river nutrient export have been incorporated into landscape biogeochemical models. Because annual export varies with precipitation, there is a need to examine the biogeochemical role of lakes and reservoirs over time frames that incorporate interannual variability in precipitation. We examined long‐term (~20 years) time series of river export (annual mass yield, Y, and flow‐weighted mean annual concentration, C) for total nitrogen (TN), total phosphorus (TP), and total suspended sediment (TSS) from 54 catchments in Wisconsin, USA. Catchments were classified as small agricultural, large agricultural, and forested by use of a cluster analysis, and these varied in lentic coverage (percentage of catchment lake or reservoir water that was connected to river network). Mean annual export and interannual variability (CV) of export (for both Y and C) were higher in agricultural catchments relative to forested catchments for TP, TN, and TSS. In both agricultural and forested settings, mean and maximum annual TN yields were lower in the presence of lakes and reservoirs, suggesting lentic denitrification or N burial. There was also evidence of long‐term lentic TP and TSS retention, especially when viewed in terms of maximum annual yield, suggesting sedimentation during high loading years. Lentic catchments had lower interannual variability in export. For TP and TSS, interannual variability in mass yield was often >50% higher than interannual variability in water yield, whereas TN variability more closely followed water (discharge) variability. Our results indicate that long‐term mass export through rivers depends on interacting terrestrial, aquatic, and meteorological factors in which the presence of lakes and reservoirs can reduce the magnitude of export, stabilize interannual variability in export, as well as introduce export time lags. Copyright © 2013 John Wiley & Sons, Ltd.     

Quantifying seasonal export and retention of nutrients in West European lowland rivers at catchment scale

To set accurate critical values for the protection of lakes and coastal areas, it is crucial to know the seasonal variation of nutrient exports from rivers. This article presents an improved method for estimating export and in‐stream nutrient retention and its seasonal variation. For 13 lowland river catchments in Western Europe, inputs to surface water and exports were calculated on a monthly basis. The catchments varied in size (21 to 486 km²), while annual in‐stream retention ranged from 23 to 84% for N and 39 to 72% for P. A novel calculation method is presented that quantifies monthly exports from lowland rivers based on an annual load to the river system. Inputs in the calculation are annual emission to the surface waters, average monthly river discharge, average monthly water temperature and fraction of surface water area in the catchment. The method accounts for both seasonal variation of emission to the surface water and seasonal in‐stream retention. The agreement between calculated values and calibration data was high (N: r² = 0·93; p < 0·001 and P: r² = 0·81; p < 0·001). Validation of the model also showed good results with model efficiencies for the separate catchments ranging from 31 to 95% (average 76%). This indicates that exports of nitrogen and phosphorus on a monthly basis can be calculated with few input data for a range of West European lowland rivers. Further analysis showed that retention in summer is higher than that in winter, resulting in lower summer nutrient concentrations than that calculated with an average annual input. This implies that accurate evaluation of critical thresholds for eutrophication effects must account for seasonal variation in hydrology and nutrient loading. Our quantification method thus may improve the modelling of eutrophication effects in standing waters. Copyright © 2011 John Wiley & Sons, Ltd.     

Nitrogen and phosphorus dynamics during runoff events over a transition from grassland to shrubland in the south‐western United States

During the last 150 years, land degradation across the semi‐arid grasslands of the south‐western United States has been associated with an increase in runoff and erosion. Concurrent with this increase in runoff and erosion is a loss of nitrogen (N) and phosphorus (P), which are plant‐essential nutrients. This study investigates the runoff‐driven redistribution and loss of dissolved and particulate‐bound N and P that occurs during natural runoff events over a trajectory of degradation, from grassland to degraded shrubland, in central New Mexico. Runoff‐driven nutrient dynamics were monitored at four stages over a transition from grassland to shrubland, for naturally occurring rainfall events over 10 × 30 m bounded runoff plots. Results show that particulate‐bound forms of N and P are responsible for most of N and P lost from the plots due to erosion occurring during runoff events. Results suggest that for high‐magnitude rainfall events, the output of N and P from the plots may greatly exceed the amount input into the plots, particularly over shrub‐dominated plots where erosion rates are higher. As these results only become apparent when monitoring these processes over larger hillslope plots, it is important to recognize that processes of nutrient cycling related to the islands of fertility hypothesis may have previously been overstated when observed only at smaller spatial scales. Thus, the progressive degradation of semi‐arid grassland ecosystems across the south‐western United States and other semi‐arid ecosystems worldwide has the potential to affect N and P cycling significantly through an increase in nutrient redistribution and loss in runoff. Copyright © 2010 John Wiley & Sons, Ltd.     

Stream exports of coarse matter and phosphorus following wildfire in NE Victoria,Australia

The temporal change in total phosphorus (TP) export from two burnt upland catchments is reported. Following wildfire in January 2003, two burnt forested headwater catchments (136 and 244 ha) in the East Kiewa valley, Victoria, were instrumented to measure discharge, turbidity and to collect stream water samples. In addition, samplers were positioned in the stream bed at the outlet of each catchment to continuously sample material transported along the bed of the stream. Approximately, every 2 weeks, the material collected by the stream bed samplers was weighed and sub‐sampled. The percentage of coarse (>1 and < 5 mm in diameter) mineral (including soil aggregates) and organic matter was determined and then analysed for TP. Between the first and third years after fire, sampled coarse matter and associated TP loads decreased by an average of 53% and 62%, respectively. Over the 3‐year study, the amount of coarse matter exported during winter/spring decreased considerably, whereas export rates during summer/autumn remained relatively constant. Coarse matter exports were estimated to be approaching pre‐fire levels after 3–4 years. Results on total suspended solids (TSS) TP and total dissolved phosphorus (TDP) from a parallel study are incorporated to explore TP partitioning. TP exported with TSS dominated the total TP export loads, with coarse matter TP and TDP each contributing approximately 10% over the study period. Copyright © 2010 John Wiley & Sons, Ltd.     

Tsunami travel times to New Zealand

A table is given for the intervals between the time that a tsunami propagating radially from a distant source first reaches a position 600 nautical miles from Wellington and the times when the tsunami first reaches any of 11 selected places in New Zealand.     

Physico‐chemical features of lake Ototoa,a sand‐dune lake in northern New Zealand

Lake Ototoa is a warm monomictic lake at 36° 31’ S, 174° 14'E. During a year's study (March 1969‐March 1970), the lake became thermally stratified in November, the metalimnion being between depths of 12 m and 16 m. Surface temperatures ranged between 10.2°c (in August) and 25.2°c (in late January), and bottom temperatures between 9.7°c and 17.5°c. The annual heat budget was calculated to be 642 354 KJ.m^‐2 (15 500 cal.cm^‐2) and the work of the wind in distributing the heat income 1.730 KJ.m^‐2 (1766 g.cm.cm^‐2). Secchi disc transparencies ranged between 5 m and 9.2 m (mean 7.07 m) and were greatest in the summer. Light transmission per metre was also high, ranging between 61% and 87%. Surface waters were normally supersaturated with oxygen, but during summer stratification oxygen concentrations in the bottom waters dropped to a minimum of 2.3 mg.litre^‐2 and a positive heterograde distribution of oxygen with depth was found. The oxygen deficit was 0.015 mg.cm^‐2.day^‐1 and showed the lake to be oligotrophic. Mean surface pH was 7.82, and the ionic composition of the waters was similar to that of other small New Zealand and Australian lakes located near the sea. Compared with other New Zealand lakes PO4‐P concentrations (range 1.00–10.20 μg.litre^‐1) were low and NO3—N concentrations (range 0.12–0.60 mg.litre^‐1) high.     

First record of Australian bonito,Sarda Australis,from New Zealand

The range of the Australian bonito, Sarda australis (Macleay, 1880), previously known only from eastern Australia and Norfolk Island, is now extended to include New Zealand. Data on two specimens examined from northern New Zealand are presented.     

Limnology of Lake Waikaremoana with special reference to littoral and pelagic primary producers

Lake Waikaremoana, the North Island's deepest lake (248 m), lies in a natural forested catchment, but the lake itself has been modified for hydro‐electric power generation and by the introduction of trout, smelt, and adventive aquatic plants. The lake is a warm monomictic water body of low conductivity (82 μS cm^‐1) and a high seasonal water column stability. The waters are oligotrophic, with epilimnetic dissolved reactive phosphorus concentrations typically < 1 mg m^‐3. The concentration of NO₃‐N is seasonally variable but generally high in winter and spring with maximum epilimnetic values approaching 70 mg m ³. This contrasts with other central North Island lakes. Horizontal variability in surface chlorophyll a is low as are the absolute values (< 1–2 mg m^‐3). A notable feature is the formation of a deep chlorophyll maximum within the metalim‐nion comprised largely of Sphaewcystis schweteri as opposed to diatoms and flagellates which normally dominate the epilimnion. Vascular macro‐phytes (maximum biomass 659 g m² dry weight) extended to 9 m and characeans (maximum biomass 447 g m²dry weight) to 16 m. Total phyto‐plankton primary production was calculated as 4524 tCy^‐1 and macrophyte production as 578 t C y^‐1. The proportion of macrophyte to phy‐toplankton production (0.14) is higher than in the other deep lakes of the central North Island.     

Relationship between tissue phosphorus and seawater phosphate in the brown alga Hormosira banksii

We measured tissue phosphorus content of high and low intertidal Hormosira banksii to test the hypothesis that tissue phosphorus content would be greater in individuals from the low intertidal because of greater total uptake associated with longer immersion in seawater. Moreover, we predicted that tissue phosphorus would be greater at sites where the seawater contained higher phosphate concentrations. There was a positive, linear relationship between local seawater phosphate concentrations and tissue phosphorus content of H. banksii from high and low intertidal zones at six different sites in winter. However, there were no comparable relationships in summer, even though the range of seawater phosphate concentration was similar in both seasons. The phosphorus contents of low intertidal H. banksii were significantly greater than high intertidal H. banksii in winter, but not in summer. Reasons for these differences may be related to greater access to seawater phosphate in low intertidal algae (than those in the high intertidal) in winter, followed by greater utilisation of the internal phosphorus between winter and summer, due to faster growth rates in the low intertidal.