塔河油田奥陶系生物扰动碳酸盐岩储集层微观孔隙结构的数字化表征与连通性分析*

塔河油田奥陶系鹰山组和一间房组发育大规模生物扰动碳酸盐岩储集层。在岩心观测和详细描述的基础上,通过岩石薄片的偏光显微镜、阴极发光显微镜观测和柱塞样品的X射线显微镜扫描与分析,实现了塔河油田奥陶系生物扰动碳酸盐岩储集层孔隙结构的数字化表征与连通性分析。结果表明: (1)由于生物扰动区潜穴充填物和围岩基质的岩石组构差异大,使这类储集层呈现极强的非均质性。(2)生物潜穴充填物主要由白云石晶体组成,白云石晶体以自形、半自形晶体为主;白云石晶间孔隙发育,孔隙体积集中于10~100μm³,喉道半径一般小于10μm,喉道长度一般小于40μm,白云石晶间孔为这类储集层的主要储集空间,围岩基质孔隙不发育。(3)生物潜穴充填物孔隙度介于7%~10%,孤立孔隙虽然数量多,但连通孔隙体积占比大,且连通孔隙拓扑结构复杂,孔隙连通性很好;通过表征单元体的尺度粗化升级分析发现,这类储集层的孔隙局部连通较好,而整体连通性相对较差。该研究真实、直观、准确刻画了生物扰动碳酸盐岩储集层微观孔隙结构特征,可为后续这类生物扰动碳酸盐岩储集层的微观渗流模拟研究提供所需的特征参数;未来将这类生物扰动碳酸盐岩储集层微观尺度上的孔隙结构数字化表征和渗流模拟结果与宏观分布规律相结合并应用到实际储集层评价中,可为这类储集层的油气资源量估算、开发方案优选、产能预测和提高采收率提供指导。     

利用Radarsat-2极化雷达数据探测湿地地表特征与分类

利用新型的Radarsat-2极化雷达数据,结合极化雷达目标分解方法提取鄱阳湖湿地不同地表类型的极化特征量,并进行了Wishart非监督和监督分类,取得了较高的精度.研究表明,Radarsat-2卫星的全天候极化雷达成像能力将在湿地监测和制图中有较大的应用潜力.     

海洋细菌QY202产κ-卡拉胶酶的分离纯化和性质研究

为获得高效降解卡拉胶菌株,从青岛太平角海域采集的角叉菜表面分离到1株高产κ-卡拉胶酶的海洋交替假单胞菌(Pseudoalteromonas sp.)QY202,经硫酸铵沉淀、脱盐、DEAE阴离子交换层析等步骤从该菌株发酵液上清中分离纯化得到1种专一性降解κ-卡拉胶的κ-卡拉胶酶,并研究了该酶的基本酶学性质.结果表明该酶被纯化了23.1倍,回收率为43.9%,分子量大小为33.2 kDa.酶的最适反应温度为40 ℃,最适反应pH为8.0,在0～40 ℃,pH=7.0～8.0之间酶活力较稳定.酶对底物κ-卡拉胶的米氏常数Km值为1.6 mg/mL.Na~+、K~+对酶活有促进作用,而Hg~(2+)、Cu~(2+)强烈抑制酶的活性.酶解κ-卡拉胶的主产物为硫酸新κ-卡拉二糖和硫酸新κ-卡拉四糖.     

圆紫菜人工色素突变体的诱导与分离

为获得圆紫菜人工色素突变体,本文使用一定剂量的紫外线辐照其叶状体,培养数天后,在叶状体上出现了少量颜色变异细胞,它们呈斑点状无规则地分布在野生型细胞中间。再培养2~3周,这些色素变异细胞分裂形成了不同颜色的细胞块,其颜色呈浅绿黄、橄榄色、草绿、绿褐、黄褐、浅褐、深紫红和浅紫红色等。在辐照强度0~80 μW/cm2范围内,叶状体上色素变异细胞块出现的频率随辐照强度的增加而增加,但增加至80 μW/cm2以上时,随着辐照强度的增加,色素变异细胞块出现的频率反而下降,这表明80 μW/cm2为有效的辐照强度。将色素变异细胞块切下,置入充气瓶内充气培养,待其释放出单孢子,随后从单孢子萌发体中挑选出了黄褐、橄榄色、红色、褐红等纯色的突变体,并利用叶状体单性生殖分别获得它们的遗传纯合丝状体（品系）。各突变品系的F₁叶状体与各自母体的颜色一样,说明其颜色是可稳定遗传的。与野生型品系相比,各色素突变品系的F₁叶状体的生长速度、活体吸收光谱、3种主要光合色素和色素蛋白的含量及它们相互间的比值均发生了明显改变。     

Relations of Rock Structure and Composition to Petrophysical and Geomechanical Rock Properties: Examples from Permocarboniferous Red-Beds

Summary. This study relates textural properties to physical and mechanical properties of coarse grained sedimentary rocks of Permocarboniferous age. As an equivalent to rock texture the principle of geomechanical order is applied. The geomechanical order describes a rock as a function of its structural and compositional order which are derived from petrological analyses. Our results indicate that rock properties like density and porosity are stronger dependent on the structural order, while strength properties additionally depend on the compositional order. The ultrasonic wave velocity responds to both structural and compositional properties. These evidences imply that the geomechanical order is not an independent parameter but a variable function of structural or compositional features, which needs specification for correlation purposes to distinct physical and mechanical rock properties.     

Purification and characterization of lectin from humoral fluids of Charybdis feriatus

1 INTRODUCTION Lectins have been regarded as having a puta- tive role in non-self recognition in vertebrate and invertebrate immunities (Arason, 1996; Matsushita, 1996; Olafsen, 1996; Vasta et al., 1999; Wilson, et al., 1999; Marques and Barracco, 2000). …     

Site assessment and evaluation of spatial earthquake ground motion of Kyeongju

The earthquake hazard has been evaluated for 10 km×10 km area around Kyeongju. The ground motion potentials were determined based on equivalent linear analysis by using the data obtained from in situ and laboratory tests. In situ tests include 16 boring investigations, 4 crosshole, 12 downhole, 26 spectral analysis of surface waves tests, and in the laboratory, resonant column tests were performed. The peak ground accelerations range between 0.141g and 0.299g on collapse level earthquake and between 0.050g and 0.120g on operation level earthquake, respectively, showing the high potential of amplification in the deep alluvial layer in Kyeongju area. Distribution maps of site amplification for the peak acceleration, amplification factors (F_a and F_v) and dominant site period of Kyeongju are constructed using geographic information system tools. The amplification factor based on the Korean seismic design guide underestimated the motion in short range and overestimated the motion in mid-period range in Kyeongju. The importance of site-specific analysis and the need for the improved site characterization method are introduced.     

Preparation and Characterization of Hydroxyiron-Montmorillonite Complexes

Iron elementin soil exists mainly in form s of Fe(OH) 2 ,Fe(OH ) 2 and Fe2 (OH ) 2 2 in tropical and subtropical areaswhere p H values are less than7.These hydroxy- Fe ions reactslowly with montmorillonite by intercalation into their inter-layer space and adsorption on their surfaces,and in this wayvarious hydroxyiron- montmorillonite complexes are formed(Cool and Vansant,1998;Wu et al.,1997;Molinared andClearfield,1994) . Hydroxyiron- montm orillonite complexesare assum ed to have…     

Analyzing Slug Tests in Wells Screened Across the Watertable: A Field Assessment

The slug test is the most widely used technique for the in situ estimation of hydraulic conductivityin confined and unconfined formations. Currently, there are no generally accepted methods in thegroundwater literature for the analysis of response data from slug tests performed in wells screenedacross the watertable. A field study was undertaken in an attempt to develop a set of practicalguidelines for tests conducted in such wells. Three wells, screened within unconsolidated materialexhibiting a range of hydraulic conductivities (.05–30.0 m/day), were installed to depths of up to9 m (30 ft) in Kansas River alluvium that ranges in thickness from 15 m to 21 m (50 ft to 70 ft)near Lawrence, Kansas. Intensive well-development efforts removed any drilling debris that couldinterfere with well-formation hydraulics. Once the wells were developed properly, a series of slugtests was performed at each well. The tests were designed to assess the role of the unsaturatedzone and the appropriateness of assuming a fixed hydraulic head upper boundary. The results ofthis investigation can be summarized as follows: (1) the sufficiency of well development shouldbe based on repeat slug tests and not the clarity of pumped water; (2) the effective screen radiusfor best model analysis should be based on a mass balance and not nominal screen dimensions;(3) the watertable can be represented as a constant head boundary and flow in the unsaturatedzone can be ignored in most situations; (4) conventional techniques for the analysis of slug-testdata seem to be reasonable for slug tests conducted in wells screened across the watertable, whenused with the appropriate effective screen radius and normalized head range; and (5) fluctuationsin the watertable elevation through time can be exploited to obtain some insight into the natureof vertical variation in hydraulic conductivity at a well. The results of this investigation indicatethat multiple slug tests should be performed at wells screened across the watertable in order toreliably assess the sufficiency of well development and the appropriateness of conventional theory.