基于高密度电阻率法的土体干缩裂隙动态发育过程精细监测研究

土体在气候作用下发育干缩裂隙是一种常见的自然现象,裂隙的存在会极大弱化土体的工程性质,诱发许多岩土与地质工程问题。为了实时掌握黏性土中干缩裂隙网络的发展状态,提出了一套基于高密度电阻率层析成像技术（ERT）的土体干缩裂隙动态发展过程精细监测方法。分别开展模型试验及原位试验,利用自行研制的测定系统持续采集电流-电位差数据,随后利用自行开发的有限元法电阻率层析成像（FemERT）系统进行数据处理,获取了裂隙网络在不同发育阶段的空间分布特征。结果表明：（1）ERT可以实现土体裂隙发育过程的精细监测,具备监测三维裂隙网络几何形态的能力,裂隙宽度的识别精度达到毫米级,裂隙深度的识别精度达到厘米级;（2）ERT的感度分布特征解释了裂隙发育对于土体电阻率的影响规律,测定电阻值时程曲线因裂隙产生位置的不同而呈现不同的变化规律;（3）反演电阻率及其相对变化率（Rev）可以直观表征裂隙网络在不同阶段的空间几何形态,凸显裂隙动态发育过程对于土体导电性的影响。     

静电场轨道阱质谱在水中溶解性有机质表征中的应用进展

溶解性有机质(Dissolved Organic Matter,DOM)在水生生态环境中广泛存在,影响着水环境中多种物质的生物地球化学性质.分析其组成与含量具有重要的生态环境意义,是生态环境科学领域研究的热点.溶解性有机质组成非常复杂,其分子组成的解析一直是难题.近年来,具有高分辨率、高灵敏度、高通量、高扫描速率等特性...     

红鳍东方鲀(Takifugu rubrips)病原杀对虾弧菌的主要生物学特性研究

采用表观分类学及分子生物学方法,对从发病的养殖红鳍东方()中分离的细菌进行了主要生物学特性研究.主要包括形态特征、理化特性等较系统的表观分类学指征鉴定;同时对代表菌株进行了16S rRNA基因的分子鉴定,测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树;结果表明,分离菌为弧茵属(Vibrio Pacini 1954)的杀对虾弧菌(Vibrio penaeicidasp.nov.Ishimaru et al 1995).代表菌株(Hc051105-6株)16s rRNA基因序列长度(不包括引物结合区)为1443bp(GenBank登录号:EU073023),代表菌株(HC051105-8株)16S rRNA基因序列长度(不包括 引物结合区)为1450bp(GenBank登录号:Eu073024).择代表菌株做对健康红鳍东方鲀的人工感染试验,认为分离鉴定的杀对虾弧菌在被检红鳍东方鱼电病例具有一定的病原学意义.药敏试验结果显示,对供试37种抗菌药物中,在一些药物中存在耐药性的菌株间差异.     

菲律宾蛤仔碱性磷酸酶的分离纯化及部分性质研究

经Tris-HCl缓冲液浸提、硫酸铵分级沉淀盐析、DEAE-Sepharose Fast Flow离子交换层析和Sephadex G-75凝胶过滤层析等步骤,从菲律宾蛤仔内脏团中分离得到碱性磷酸酶,SDS-PAGE显示为单一条带,提纯倍数为14.42,比活力达到131.08U/mg。酶学性质研究表明:该酶亚基分子量约44kD,最适反应温度为45℃,最适pH值为9.0,米氏常数Km为0.098mmol/L,最大反应速度Vmax为1.01μmol/(mL·min)。Ca2+、Mg2+对酶活力表现出显著的激活作用。     

XX盆地低渗储层束缚水饱和度计算方法研究

束缚水饱和度的大小是划分油气层与水层的重要依据之一.在岩石物理实验的基础上,分析影响束缚水饱和度大小的各种因素,利用彼此之间的关系求取束缚水饱和度的大小.以XX盆地低渗储层为例,分析低渗储层束缚水饱和度的特点,建立了束缚水饱和度与孔隙度、渗透率、气柱高度等的模型,应用到实际资料中,检验各种模型的适用性与准确性,取得了较好的效果.     

Controls on reservoir heterogeneity of tight sand oil reservoirs in Upper Triassic Yanchang Formation in Longdong Area,southwest Ordos Basin,China: Implications for reservoir quality prediction and oil accumulation

Compared to conventional reservoirs, pore structure and diagenetic alterations of unconventional tight sand oil reservoirs are highly heterogeneous. The Upper Triassic Yanchang Formation is a major tight-oil-bearing formation in the Ordos Basin, providing an opportunity to study the factors that control reservoir heterogeneity and the heterogeneity of oil accumulation in tight oil sandstones.The Chang 8 tight oil sandstone in the study area is comprised of fine-to medium-grained, moderately to well-sorted lithic arkose and feldspathic litharenite. The reservoir quality is extremely heterogeneous due to large heterogeneities in the depositional facies, pore structures and diagenetic alterations. Small throat size is believed to be responsible for the ultra-low permeability in tight oil reservoirs. Most reservoirs with good reservoir quality, larger pore-throat size, lower pore-throat radius ratio and well pore connectivity were deposited in high-energy environments, such as distributary channels and mouth bars. For a given depositional facies, reservoir quality varies with the bedding structures. Massive- or parallel-bedded sandstones are more favorable for the development of porosity and permeability sweet zones for oil charging and accumulation than cross-bedded sandstones.Authigenic chlorite rim cementation and dissolution of unstable detrital grains are two major diagenetic processes that preserve porosity and permeability sweet zones in oil-bearing intervals. Nevertheless, chlorite rims cannot effectively preserve porosity-permeability when the chlorite content is greater than a threshold value of 7%, and compaction played a minor role in porosity destruction in the situation. Intensive cementation of pore-lining chlorites significantly reduces reservoir permeability by obstructing the pore-throats and reducing their connectivity. Stratigraphically, sandstones within 1 m from adjacent sandstone-mudstone contacts are usually tightly cemented (carbonate cement > 10%) with low porosity and permeability (lower than 10% and 0.1 mD, respectively). The carbonate cement most likely originates from external sources, probably derived from the surrounding mudstone. Most late carbonate cements filled the previously dissolved intra-feldspar pores and the residual intergranular pores, and finally formed the tight reservoirs.The petrophysical properties significantly control the fluid flow capability and the oil charging/accumulation capability of the Chang 8 tight sandstones. Oil layers usually have oil saturation greater than 40%. A pore-throat radius of less than 0.4 μm is not effective for producible oil to flow, and the cut off of porosity and permeability for the net pay are 7% and 0.1 mD, respectively.     

斑鳢(Channa maculata)微囊藻毒素去毒相关基因sGST的克隆与序列分析

采用RT-PCR技术和RACE技术成功克隆淡水鱼类斑鳢sGST基因cDNA全序列,推测得到氨基酸序列,初步分析其结构功能域及系统进化关系。结果表明,斑鳢sGST基因cDNA序列全长为898bp,编码225个氨基酸。斑鳢sGST与真鲷、金头鲷、鲽、黑头鲦、川鲽、大口黑鲈等最新定名为ρ型的sGST氨基酸同源性较高,ρ型sGST为水生生物所特有并共同占据进化树上独立的分枝;与大鼠、小鼠、人等哺乳动物sGST现有所有类型同源性均很低,并且在进化树上距离也较远,表明本研究成功克隆的sGST基因应属于ρ型,可能在鱼类等水生生物对水栖环境的适应上有重要作用。     

GC-MS联合二维核磁对文蛤多糖Fr.2A组分结构的解析

采用GC-MS联合二维核磁的方法,对文蛤均多糖结构进行解析。运用双酶辅助水提的方法,提取文蛤粗多糖(MMPX),分离纯化得到文蛤均多糖,并通过气质联用技术(GC-MS)和核磁共振波谱(1D,2D NMR),对文蛤均多糖的结构进行了分析。结果表明,从文蛤中提取得到文蛤粗多糖(MMPX),经分离纯化得到高纯度文蛤均多糖Fr.2A,其单糖组成为葡萄糖(Glc)。GC-MS分析发现,Fr.2A存在三种残基单元1,4-Glcp、T-Glcp和1,4,6-Glcp,摩尔比为5.58︰1.26︰1.0;核磁共振波谱分析表明,Fr.2A是以α-(1→4)与少量α-(1→6)连接为主链,并存在少量的α-(1→6)和β-(1→4)支链的新型水溶性D型吡喃葡聚糖。     

Statistical facies classification from multiple seismic attributes: comparison between Bayesian classification and expectation–maximization method and application in petrophysical inversion

We present here a comparison between two statistical methods for facies classifications: Bayesian classification and expectation–maximization method. The classification can be performed using multiple seismic attributes and can be extended from well logs to three‐dimensional volumes. In this work, we propose, for both methods, a sensitivity study to investigate the impact of the choice of seismic attributes used to condition the classification. In the second part, we integrate the facies classification in a Bayesian inversion setting for the estimation of continuous rock properties, such as porosity and lithological fractions, from the same set of seismic attributes. The advantage of the expectation–maximization method is that this algorithm does not require a training dataset, which is instead required in a traditional Bayesian classifier and still provides similar results. We show the application, comparison, and analysis of these methods in a real case study in the North Sea, where eight sedimentological facies have been defined. The facies classification is computed at the well location and compared with the sedimentological profile and then extended to the 3D reservoir model using up to 14 seismic attributes.     

联合多点地质统计学与序贯高斯模拟的随机反演方法

岩相和储层物性参数是油藏表征的重要参数,地震反演是储层表征和油气藏勘探开发的重要手段.随机地震反演通常基于地质统计学理论,能够对不同类型的信息源进行综合,建立具有较高分辨率的储层模型,因而得到广泛关注.其中,概率扰动方法是一种高效的迭代随机反演策略,它能综合考虑多种约束信息,且只需要较少的迭代次数即可获得反演结果.在概率扰动的优化反演策略中,本文有效的联合多点地质统计学与序贯高斯模拟,并结合统计岩石物理理论实现随机反演.首先,通过多点地质统计学随机模拟,获得一系列等可能的岩相模型,扰动更新初始岩相模型后利用相控序贯高斯模拟建立多个储层物性参数模型;然后通过统计岩石物理理论,计算相应的弹性参数;最后,正演得到合成地震记录并与实际地震数据对比,通过概率扰动方法进行迭代,直到获得满足给定误差要求的反演结果.利用多点地质统计学,能够更好地表征储层空间特征.相控序贯高斯模拟的应用,能够有效反映不同岩相中储层物性参数的分布.提出的方法可在较少的迭代次数内同时获得具有较高分辨率的岩相和物性参数反演结果,模型测试和实际数据应用验证了方法的可行性和有效性.