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791.
The neutralizing activities of eight monoclonal antibodies (MAbs) against white spot syndrome virus (WSSV) (2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4) were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs (hybridoma culture supernatant), respectively. The mixture of WSSV and MAbs were injected into crayfish (Procambarus clarkii). After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions. 相似文献
792.
Surface current field and seasonal variability in the Kuroshio and adjacent regions derived from satellite-tracked drifter data 总被引:7,自引:2,他引:5
The muhiyear averaged surface current field and seasonal variability in the Kuroshio and adjacent regions are studied. The data used are trajectories and (1/4) ° latitude by (1/4) ° longitude mean currents derived from 323 Argos drifters deployed by Chinese institutions and world ocean circulation experiment from 1979 to 2003. The results show that the Kuroshio surface path adapts well to the western boundary topography and exhibits six great turnings. The branching occurs frequently near anticyclonic turnings rather than near cyclonic ones. In the Luzon Strait, the surface water intrusion into the South China Sea occurs only in fall and winter. The Kuroshio surface path east of Taiwan, China appears nearly as straight lines in summer, fall, and winter, when anticyclonic eddies coexist on its right side; while the path may cyclonically turning in spring when no eddy exists. The Kuroshio intrusion northeast of Taiwan often occurs in fall and winter, but not in summer. The running direction, width and velocity of the middle segment of the Kuroshio surface currents in the East China Sea vary seasonally. The northward intrusion of the Kuroshio surface water southwest of Kyushu occurs in spring and fall, but not in summer. The northmost position of the Kuroshio surface path southwest of Kyushu occurs in fall, but never goes beyond 31 °N. The northward surface current east of the Ryukyu Islands exists only along Okinawa-Amami Islands from spring to fall. In particular, it appears as an arm of an anti- cyclonic eddy in fall. 相似文献
793.
利用南极走航观测评估卫星遥感海表面温度 总被引:3,自引:1,他引:2
利用1989-2005年间南极走航观测的海表面温度,对目前3个主要的卫星反演的SST产品AVHRR(Advanced Very High Resolution Radiometer),TMI(TRMM Microwave Imager)和AMSR-E(Advanced Microwave Scanning Radiometer for the Earth Observing System)进行了较为系统的评估,并着重检验了它们在南大洋的准确性.结果表明,AVHRR SST比观测数据偏冷,白天的偏差为-0.12℃,夜晚的偏差为-0.04℃,而且南大洋的冷偏差更为显著.TMI SST比观测数据明显偏暖,白天的偏差为0.48℃,夜晚的偏差为0.57℃,其温差ΔT受37GHz风速影响,在强风速(>6m/s)下这种影响仍然存在.AMSR-ESST比观测数据偏暖,白天的偏差为0.34℃,夜晚的偏差为0.27℃,而且南大洋的暖偏差相对较大.AMSR-E SST温差受水汽影响,并在南大洋随着水汽的增加而增加.通过进一步比较微波(AMSR-E和TMI)和红外(AVHRR)遥感的SST在2004年北半球冬季(即南半球夏季)的差别,发现微波遥感在热带(15°S-15°N)和南大洋区域(45°S以南)比红外遥感偏暖,而且在南大洋区域的偏差相对较大,相反在北半球中纬度区域(15°~40°N)偏冷.AMSR-E与AVHRR SST的温差,从白天到夜晚有减小的趋势,而TMI与AVHRR SST的温差无明显的变化. 相似文献
794.
Takashi Doi Michiaki Takano Kei Okamura Tamaki Ura Toshitaka Gamo 《Journal of Oceanography》2008,64(3):471-477
We present the results of a chemical survey at a submarine volcano, Teishi Knoll, obtained using a submersible in-situ Mn
analyzer (GAMOS) mounted on an autonomous underwater vehicle (AUV) platform. During this survey, high-resolution data of dissolved
Mn were obtained in three dimensions in nearly real time. The AUV enabled continuous detailed observations along a preprogrammed
pathway, which could not have been performed with conventional CTD hydrocasts. During this observation, anomalously high dissolved
Mn concentrations were obtained within the crater, corresponding to high water temperatures. The anomalies might be a hydrothermal
signature due to volcanic activity from the crater. 相似文献
795.
原位杂交技术在斜带石斑鱼神经坏死病毒检测中的应用 总被引:4,自引:0,他引:4
根据斜带石斑鱼(Epinephelus coioids)神经坏死病毒(orang-spotted nervous necrosis virus,0GNNV)的主衣壳蛋白(major capsid protein,MCP)基因的保守序列,设计一对引物,从感染0GNNV的斜带石斑鱼组织匀浆液提取RNA为模板进行RT-PCR扩增,得到426bp的cDNA片断。用得到的RT-PCR产物加上地高辛(DIG)标记作为核酸探针。通过注射病毒提取液人工感染一组斜带石斑鱼,解剖感染病毒的斜带石斑鱼,从中分离出脑和眼睛,运用原位杂交技术检测组织中的OGNNV。实验表明,原位杂交具有较高的灵敏性和特异性,可以用原位杂交的办法来检测养殖的石斑鱼是否有携带该病毒,达到监控和预防神经坏死病爆发的目的。本实验还采用了H&E染色方法检测了感染NNV的石斑鱼脑部和眼部组织,观察细胞内的坏死部分。与原位杂交做对比,提高了检测的可靠性和准确性。本实验建立的石斑鱼神经坏死病毒的ISH检测方法有着较高的特异性和敏感性,易于操作,有助于石斑鱼神经坏死病毒的组织定位、发病机理的研究。 相似文献
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