山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

关于南海暖水季节和年际变化的研究

阐述了研究南海暖水的意义 ,综述了关于南海暖水的现状 ,提出了关于南海暖水季节和年际变化方面应该研究的问题     

热带大西洋年际和年代际变率的时空结构模拟

使用美国夏威夷大学发展的中等复杂程度海洋模式(IOM)在给定表面强迫条件下模拟了热带大西洋上层海洋年际和年代际变率的时空结构.利用NCEP的41a(1958～1998年)逐月平均表面资料作为强迫场,积分海洋模式41a作为控制试验,并利用模式分别做动量(风应力)通量和热量通量无异常变化的平行试验,与控制试验作比较.对3组试验模拟上层海洋变率状况的比较,并按年际和年代际时间尺度分别分析,揭示表面风应力和热通量异常对海表面温度和温跃层深度变化的影响,并比较了其影响的相对重要性.结果表明模式成功地模拟出了热带大西洋上层海洋的变率.模式模拟的海表面温度年际变化主要表现为弱ENSO型,年代际变化表现为南、北大西洋变化相反的偶极子型.在年际时间尺度上,热力强迫和动力强迫对海表温度变化都有贡献,其中赤道外海表面温度异常(SSTA)变化主要由热通量异常引起,而近赤道SSTA的变化主要由动量异常强迫引起.在年代际时间尺度上,热通量强迫的作用远比动量强迫重要.模式不仅能够模拟SST在年际和年代际时间尺度上的变率,还能够模拟温跃层深度在年际和年代际时间尺度上的变率.年际和年代际时间尺度上,温跃层深度的变率主要由动量异常决定,热通量异常强迫的贡献很小.     

Oxygen Uptake with Acute Variation in Temperature and Salinity in Two Coral Reef Polychaetes

Abstract. Eupolymnia nebulosa (Terebellidae) and Hermodke carunculata (Amphinomidae) were compared with respect to rates of oxygen consumption in response to acute temperature and salinity variation. E. nebulosa , but not H. carunculata , demonstrated significant temperature sensitivity correlating with the former species' restriction to habitats that are temperature variation. In contrast, H. carunculata occurs where such fluctuation is common. Oxygen uptake in both species was responsive to dilution of the medium whereas only H. carunculata demonstrated insensitivity to increased salinity under most temperature conditions.     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

Drag Coefficient,Dynamic Roughness and Reference Wind Speed

Surface waves are the roughness element of the ocean surface. The parameterization of the drag coefficient of the ocean surface is simplified by referencing to wind speed at an elevation proportional to the characteristic wavelength. The dynamic roughness is analytically related to the drag coefficient. Under the assumption of fetch limited wave growth condition, various empirical functions of the dynamic roughness can be converted to equivalent expressions for comparison. For datasets covering a wide range of the dimensionless frequency (inverse wave age), it is important to account for the variable rate of wave development at different wave ages. As a result, the dependence of the Charnock parameter on wave age is nonmonotonic. Finally, the analysis presented here suggests that the significant wave steepness is a sensitive property of the ocean surface and a single variable normalization of the dynamic roughness using a wavelength or wave height parameter actually produces more robust functions than bi-variable normalizations using wave height and wave slope.     

Influence of Current Width Variation on the Annual Mean Transport of the East Sakhalin Current: A Simple Model

Recent observations suggest that the annual mean southward transport of the East Sakhalin Current (ESC) is significantly larger than the annual mean Sverdrup transport. Motivated by this observational result, transport of a western boundary current has been investigated using a simple numerical model with a western slope. This transport is defined as the instantaneous barotropic transport integrated from the western boundary to the offshore point where the barotropic velocity vanishes. The model, forced by seasonally varying wind stress, exhibits an annual mean of the western boundary current transport that is larger than that of the Sverdrup transport, as observed. The southward transport from October to March in the model nearly equals the instantaneous Sverdrup transport, while the southward transport from April to September decreases slowly. Although the Sverdrup transport in July vanishes, the southward transport in summer nearly maintains the annual mean Sverdrup transport, because the barotropic Rossby wave cannot intrude on the western slope. This summer transport causes the larger annual mean. Although there are some uncertainties in the estimation of the Sverdrup transport in the Sea of Okhotsk, the seasonal variation of the southward transport in the model is qualitatively similar to the observations.     

太平洋海域海平面变化的灰色系统分析

应用灰色系统理论，对太平洋海域４８个长期验潮站的月均海平面分别建立了ＧＭ（１，１）模型。ＧＭ（１，１）模型能较好地反映太平洋海域的海平面变化的趋势，它除了能给出连续的海平面变化速率外，同时能方便地给出海平面变化的加速率。模拟结果表明，在太平洋地壳均衡假设下，太平洋海域的月均海平面以平均速率０．１７ｃｍ／ａ上升。在太平洋海域所取的４８个长期验潮站中，有４０个站在加速上升，全部站的平均加速度为０．０００２９ｃｍ／ａ２。且加速率逐渐增大。当然这些加速率都很小，但作为一种普遍性的趋势，这已足以说明：太平洋海域的海平面在加速上升     

中国近海鱼类生活史型与生态学参数地理变异

根据1985－1986年东海北部近海调查材料及其它有关文献，对43种鱼计74个种群或群体的生活史选择型及其生态参数进行了比较研究。结果表明，分布于我国近海的主要鱼类大多偏于r选择型。对r－K选择鱼种的资源特征及其对捕捞的反应作了阐述。各鱼种的种群生态学参数在种间和空间分布均表现出地理变异。     

中华乌塘鳢的群体生物学研究 Ⅱ.生化遗传变异

用同工酶谱法发现中华乌塘鳢Bostrichthy sinensis四个群体的遗传变异水平很低,在所检测的30个基因座位中,多态座位比例只有0—10％,平均杂合度仅为0.2—0.5％,物种的平均杂合度为0.33±0.10％。四个群体共享常见的等位基因。虽然其中三个群体拥有特有的等位基因,但其基因频率都未超过5％。四个群体的Nei氏遗传距只有1.68—12.13×10~(-5)。据分析,近交可能是遗传变异水平低和形态特征分化差的主要原因。种苗生产上应注意广泛采集不同地理群体的鱼作亲鱼,以丰富养殖群体的基因库。