荧光分光光度法测定海洋生物中的谷胱甘肽

对应用荧光分光光度法测定海洋生物中还原型谷胱甘肽(GSH)含量的方法进行了研究,并测定了分属于鱼、虾、贝、藻的10种海洋生物中谷胱甘肽的含量。利用邻苯二甲醛与GSH反应构成的荧光体系,在激发波长为365nm,发射波长为425nm的条件下,方法的回收率为99.22%~99.69%,变异系数为2.16%。应用此方法测得10种海洋生物中谷胱甘肽的含量为:红笛鲷(Lutjanussanguineus)0.399mg/g,银鲳(Pampusargenteus)0.352mg/g,大海鲢(Megalopscyrinoides)0.561mg/g,尖紫蛤(Sanguinolariaacuta)0.289mg/g,菲律宾蛤仔(Ruditapesphilippinarun)0.287mg/g,墨吉对虾(Penaeusmerguiensis)0.892mg/g,凡纳滨对虾(Litopenaeusvannamei)1.434mg/g,囊藻(Colpomeniasinu-ose)0.221mg/g,石莼(Ulvalactucal)0.727mg/g,马尾藻(Sargassummuticum)0.137mg/g。     

Orbital linkages of Comet 6P/d'Arrest based on its asymmetric light curve

We present an investigation of different models of the nongravitational acceleration on Comet 6P/d'Arrest, as used in orbital linkages spanning 150 years from the discovery of the comet in 1851 until the recent observations made in 2001. Some of our models use the time-shifted g-like function to represent the variation of outgassing rate, but the main thrust is on models using instead a production curve that is fitted to recent light curve observations—mainly those in 1976. We pay special attention to the proper scaling of such a production curve, when applied to other apparitions with a different perihelion distance q, and we find a best fit with a q^−1.6 power-law. Generally, the best fit is found with models, in which the acceleration components are expressed in terms of the angular parameters of the rotating nucleus. We thus find the orientation of the spin axis, and using the orbital evolution we are able to predict a variable time shift of the outgassing curve. The very best results are found when applying this time shift to the light-curve based, angular models. The totality of the 1851-2001 observations can then be linked with a mean residual of less than 4″. This may be brought down to ∼^2″ by solving for individual ‘activity parameters’ of all apparitions, which are multiplicative factors applied to the acceleration amplitudes. These turn out to be within 10% of unity for the best fit. We have also performed a linkage to the observations of Comet 1678 (La Hire) using our models. We find an indication of a secular increase of the amount of asymmetry of the outgassing with respect to perihelion, part of which is due to the variable time shift caused by the orbital evolution.     

SHORT COMMUNICATION APPLICATION OF THE POWELL METHOD TO THE OPTIMIZATION OF FLOW INJECTION SYSTEMS

This paper describes the application of the Powell algorithm to the optimization of a flow injectionsystem configuration.The performance of this algorithm has been compared with the modified simplexmethod.The system studied is the determination of ammonia,based on the indophenol blue reaction.Alinear combination of sensitivity and sample throughput is used as the objective function because of itssimultaneous optimization capability.Results obtained show that the proposed method may reach theoptimal conditions with a lower number of experimental evaluations.     

Synthetically adaptive robust filtering for satellite orbit determination

The precise orbit information is the premise of the application of satellite, and the precise orbit deter- mination includes dynamic and geometric methods. The dynamic method estimates the position and veloc-ity of a satellite at one epoch by extending the obser-vational arc in order to include more observations of different epochs in it. It is realized by integrating the dynamic equation of the satellite so that the observa-tions at different epochs are related to the particular state. The dy…     

Atmospheric densities derived from CHAMP/STAR accelerometer observations

The satellite CHAMP carries the accelerometer STAR in its payload and thanks to the GPS and SLR tracking systems accurate orbit positions can be computed. Total atmospheric density values can be retrieved from the STAR measurements, with an absolute uncertainty of 10-15%, under the condition that an accurate radiative force model, satellite macro-model, and STAR instrumental calibration parameters are applied, and that the upper-atmosphere winds are less than . The STAR calibration parameters (i.e. a bias and a scale factor) of the tangential acceleration were accurately determined using an iterative method, which required the estimation of the gravity field coefficients in several iterations, the first result of which was the EIGEN-1S (Geophys. Res. Lett. 29 (14) (2002) 10.1029) gravity field solution. The procedure to derive atmospheric density values is as follows: (1) a reduced-dynamic CHAMP orbit is computed, the positions of which are used as pseudo-observations, for reference purposes; (2) a dynamic CHAMP orbit is fitted to the pseudo-observations using calibrated STAR measurements, which are saved in a data file containing all necessary information to derive density values; (3) the data file is used to compute density values at each orbit integration step, for which accurate terrestrial coordinates are available. This procedure was applied to 415 days of data over a total period of 21 months, yielding 1.2 million useful observations. The model predictions of DTM-2000 (EGS XXV General Assembly, Nice, France), DTM-94 (J. Geod. 72 (1998) 161) and MSIS-86 (J. Geophys. Res. 92 (1987) 4649) were evaluated by analysing the density ratios (i.e. “observed” to “computed” ratio) globally, and as functions of solar activity, geographical position and season. The global mean of the density ratios showed that the models underestimate density by 10-20%, with an rms of 16-20%. The binning as a function of local time revealed that the diurnal and semi-diurnal components are too strong in the DTM models, while all three models model the latitudinal gradient inaccurately. Using DTM-2000 as a priori, certain model coefficients were re-estimated using the STAR-derived densities, yielding the DTM-STAR test model. The mean and rms of the global density ratios of this preliminary model are 1.00 and 15%, respectively, while the tidal and latitudinal modelling errors become small. This test model is only representative of high solar activity conditions, while the seasonal effect is probably not estimated accurately due to correlation with the solar activity effect. At least one more year of data is required to separate the seasonal effect from the solar activity effect, and data taken under low solar activity conditions must also be assimilated to construct a model representative under all circumstances.     

利用中心差分法进行GPS定速时最佳点数的选取

分析了利用中心差分法进行GPS定速时的主要误差来源,证明了当数据采样率一定时,增加中心差分法的点数可减少微分过程中的截断误差,但同时会放大导出相位率的观测误差,得出中心差分法的最佳点数应使这两种误差之和最小的结论。实验结果表明,当数据采样间隔为1s、载体平均速度和加速度为20m.s-1和0.2m.s-2时,9个点的中心差分法定速精度最高。     

利用C波段转发数据实现GEO卫星轨道确定

为了提高我国卫星导航系统GEO卫星定轨能力,针对我国卫星导航系统所特有的新数据和新方法,利用CAPS系统的C波段转发式测距数据进行了单GEO卫星定轨实验.在分析该方法技术特点的基础上,从定轨残差和轨道重叠弧段比较等方面,对定轨结果进行了分析.由于设备转发时延是影响该方法定轨精度的重要因素,因此同时还分析了系统偏差对定轨精度的影响,得出了一些有益的结论.     

利用GPS伪距导出的多普勒频移测速

推导了利用伪距观测值获取多普勒频移的公式,并利用导出的多普勒频移来确定载体的速度。实测数据表明,利用伪距导出的多普勒频移测速,可以达到dm/s级的水平。在没有原始多普勒观测值或者相位观测出现了频繁周跳的情况下,可以利用伪距导出的多普勒频移获得载体概略的速度信息。     

云的立体观测研究进展

对云的立体观测的研究历史做了回顾,按照具有代表性的观测平台的变化划分了4个发展阶段,分别予以介绍,内容包括典型平台、技术途径和特点、同步精度、云高指定精度等。重点介绍了双星联合观测的云高指定技术,分析了关键技术和可能存在的问题,为技术实现做了较好的准备。