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131.
固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定 总被引:3,自引:0,他引:3
酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶(AChE).制备方法为:0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高(96%),对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备. 相似文献
132.
Application of LICOM to the numerical study of the water exchangebetween the South China Sea and its adjacent oceans 总被引:8,自引:1,他引:8
1 IntroductionThe South China Sea (SCS) is the largestmarginal sea in the western Pacific (see Fig. 1). It con-nects with the SCS through the Taiwan Strait, with thePacific through the Luzon Strait, with the Sulu Seathrough the Mindoro and Balabac Straits and with theJava Sea and Andaman Sea through the Sunda Shelf(For convenience, here we refer to the section at 1.5°N,Fig. 2). It is shown that the seasonal SCS circulation ismostly affected by the summer/winter monsoon, andthe no… 相似文献
133.
Mohamed?IsmailEmail author Tadashi?Kimura Yumiko?Suzuki Makoto?Tsuchiya 《Journal of Oceanography》2005,61(3):631-644
Total mass flux, size distribution of sediment particles and some chemical components such as total carbon (TC), total nitrogen (TN) and calcium carbonate (CaCO3) were monitored monthly using a multi-cup sediment traps at seven coral reef sites (6 reef flat and 1 reef slope) of the Marine Protected Areas around Ishigaki, Kohama, Kuroshima and Iriomote Islands in the southern Ryukyus, Japan from September 2000 to September 2001. The size distribution of trapped sediments revealed mostly uni-modal fine sand to mud in the reef flat and gravelly to coarse sand in the reef slope. The total mass flux ranged between 0.54 to 872 gm−2d−1, and showed a pronounced seasonality (high in summer-autumn and low in spring) at each site, which was consistent with the rainfall and typhoon regime. Exceptionally high values were observed on the reef slope (Iriomote) in February–March 2001 (1533 gm−2d−1) owing to a large amount of bottom sediment re-suspension. On the reef flat (Todoroki South and North; Ishigaki), values obtained in July–August 2001 (872 gm−2d−1) and August–September 2001 (800 gm− 2d−1) indicate the high terrestrial discharge from Todoroki River. Trapped sediment particles consist of CaCO3 (1.2–27.1%) and a non-carbonate fraction (98.8–72.9%), which contains total carbon (4.9–26%), carbonate carbon (CO2-C) (0.2–3.1%) and non-carbonate carbon (NC-C) (7.9–25.6%). Total nitrogen content was in the range 0.02–0.48%. TN is contained mainly in the carbonate fraction and NC-C may be contained in the non-carbonate fraction. The low TN/OC ratio of the trapped sediments suggests that they were mostly of terrestrial origin and that both fractions migrated. The high total mass flux derived from Todoroki River exceeded the threshold at which a lethal effect on coral community is caused. The results stress the importance of conducting seasonal studies of sedimentation over more than one year and at more than one location in south Japan coral reef ecosystems to gain an understanding of the processes controlling the total mass fluxes and their nutrients content, also to develop an awareness of how to prevent the damage of coral reef ecosystems and, if it does occur, to allow mitigation measures to be undertaken. 相似文献
134.
135.
顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究 总被引:12,自引:2,他引:12
建立了顶空GC/FPD测定海水中二甲基硫(DMS)和浮游植物细胞中二甲基硫丙酸(DMSP)的方法,并研究盐度、温度、气液相比DMS诸因素对DMS顶空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6%,平均回收率为106%,最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS,在50℃下作用时间不少于6h,峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾 相似文献
136.
137.
SeaMARC II sidescan (imagery and bathymetry) and seismic data reveal the morphology, sedimentary processes, and structural controls on submarine canyon development in the central Izu-Bonin forearc, south of Japan. Canyons extend up to 150 km across the forearc from the trench-slope break to the active volcanic arc. The canyons are most deeply incised (1200–1700 m) into the gentle gradients (1–2°) upslope on the outer arc high (OAH) and lose bathymetric expression on the steep (6–18°) inner trench-slope. The drainage patterns indicate that canyons are formed by both headward erosion and downcutting. Headward erosion proceeds on two scales. Initially, pervasive small-scale mass wasting creates curvilinear channels and pinnate drainage patterns. Large-scale slumping, evidenced by abundant crescent-shaped scarps along the walls and tributaries of Aoga Shima Canyon, occurs only after a channel is present, and provides a mechanism for canyon branching. The largest slump has removed >16 km3 of sediment from an 85 km2 area of seafloor bounded by scarps more than 200 m high and may be in the initial stages of forming a new canyon branch. The northern branch of Aoga Shima Canyon has eroded upslope to the flanks of the arc volcanoes allowing direct tapping of this volcaniclastic sediment source. Headward erosion of the southern branch is not as advanced but the canyon may capture sediments supplied by unconfined (non-channelized) mass flows.Oligocene forearc sedimentary processes were dominated by unconfined mass flows that created sub-parallel and continuous sedimentary sequences. Pervasive channel cut-and-fill is limited to the Neogene forearc sedimentary sequences which are characterized by migrating and unconformable seismic sequences. Extensive canyon formation permitting sediment bypassing of the forearc by canyon-confined mass flows began in the early Miocene after the basin was filled to the spill points of the OAH. Structural lows in the OAH determined the initial locus of canyon formation, and outcropping basement rocks have prevented canyon incision on the lower slope. A major jog in the canyon axis, linear tributaries, and a prominent sidescan lineament all trend NW-NNW, reflecting OAH basement influence on canyon morphology. This erosional fabric may reflect joint/fracture patterns in the sedimentary strata that follow the basement trends. Once the canyons have eroded down to more erosion-resistant levels, channel downcutting slows relative to lateral erosion of the canyon walls. This accounts for the change from a narrow canyon axis in the thickly sedimented forearc basin to a wider, more rugged canyon morphology near the OAH. About 9500 km3 of sediment has been eroded from the central, 200 km long, segment of the Izu-Bonin forearc by the formation of Aoga Shima, Myojin Sho and Sumisu Jima canyons. The volume of sediment presently residing in the adjacent trench, accretionary wedge, and lower slope terrace basin accounts for <25% of that eroded from the canyons alone. This implies that a large volume (>3500 km3 per 100 km of trench, ignoring sediments input via forearc bypassing) has been subducted beneath the toe of the trench slope and the small accretionary prism. Unless this sediment has been underplated beneath the forearc, it has recycled arc material into the mantle, possibly influencing the composition of arc volcanism. 相似文献
138.
对虾养殖生态系中有机碳的初步研究本研究由 总被引:1,自引:0,他引:1
对于围隔养殖对虾生态系中有机碳的变动进行了研究 ,结果表明 :溶解有机碳 (DOC)含量波动在 5 .2 99~ 13.39mg/ L之间 ,平均为 8.5 3mg/ L± 2 .2 5 mg/ L;颗粒有机碳 (POC)含量波动在0 .6 5~ 6 .6 3mg/ L之间 ,平均为 3.2 5 mg/ L± 1.76 mg/ L;总有机碳 (TOC)含量波动在 6 .92~ 2 0 .0 2mg/ L之间 ,平均为 11.78mg/ L± 3.82 mg/ L ;其中 DOC∶ POC∶ TOC为 0 .72∶ 0 .2 8∶ 1。各种有机碳组分的含量明显高于自然海水中各种有机碳的含量 ;各种有机碳组分的含量在养殖期间总体上呈上升的趋势 ;其变化与 DCOD的变化呈显著线性正相关的关系。 相似文献
139.
九龙江河口区水体中的226Ra和228Ra 总被引:8,自引:0,他引:8
用MnO2-纤维富集大体积水样中的Ra同位素,并用直接射气法测定226Ra和228Ac的β计数法测定228Ra的方法,研究了九龙江河口区226Ra,228Ra的行为,结果表明:在该河口区226Ra,228Ra均呈非保守行为,226Ra,228Ra的最高值分别在盐度为19和9处测得。228Ra/226Ra)A.R.值在盐度小于19的区域约为3左右。与世界其他河口区相比,九龙江河口区的226Ra,22 相似文献
140.
根据1988年7月调查资料讨论了台湾海峡中北部海域夏季上升流区各种形态磷的分布特征。结果表明,上升流中心区(即海坛岛东侧一带水域)具有低温、高盐、低溶解氧;DIp,TDP,PP,TP较高;DOP较低(0.27);及DIP/DOP比值(约为1)较高的特性。DOP是该海域磷的主要形态(占TP的49.2%),其含量分布及其形态转化与生物活动直接相关,是该海域生物生产力高的体现。 相似文献