华贵栉孔扇贝四种壳色选育系F1遗传多样性的AFLP分析

用10 对AFLP 选择性扩增引物, 对4 种壳色华贵栉孔扇贝(Chlamys nobilis)选育系的F₁代群体(每种壳色30 个, 共120 个个体)进行了遗传多样性分析, 共扩增出414 个位点, 多态性位点352 个, 比例为85.02%; 紫顶枣褐、枣褐、紫白、橘黄4 种壳色群体多态性位点比例分别为72.83%、86.04%、83.47%、67.35%; Nei’s基因多样性指数(H)分别为0.3071、0.3494、0.3358、0.3077; Shannon’s 信息指数(I)分别为0.4607、0.5139、0.4987、0.4525。4 个群体间的平均遗传分化系数(G_st)为0.1739, 遗传杂合度(H_e)为0.3954, 遗传距离(D)变化范围在0.1194～0.1720 之间; 聚类分析表明, 4 种壳色选育系间的亲缘关系由近及远依次为紫白、枣褐、橘黄和紫顶枣褐壳色。研究表明, 四种壳色选育系均具有较高的遗传多样性, 各壳色群体间存在一定分化, 继续选育潜力大。     

长牡蛎(Crassostrea gigas)贝壳与外套膜中黑色素的提取和鉴定

为了提取和鉴定长牡蛎(Crassostrea gigas)贝壳与外套膜中的黑色物质,选取贝壳与外套膜颜色均为黑色的长牡蛎,将贝壳及外套膜进行粉碎、盐酸水解、水浴加热、乙醚抽脂,从3份贝壳中提取出3份黑色固体物质,从3份外套膜中提取出3份黑色固体物质,将提取的黑色固体溶解于0.01m L/L氢氧化钠水溶液中,在150—500 nm的范围内测定紫外吸收光谱,发现其最大吸收峰在210 nm左右,随着波长增加,其吸光值迅速大幅度下降,与公认的黑色素紫外吸收光谱特征一致,从而首次确定长牡蛎贝壳与外套膜中的黑色物质为黑色素。     

水下环肋圆柱壳弹性失稳临界载荷无损预报方法

基于振动的水下环肋圆柱壳临界载荷的预报具有结构无损的优势。以Flügge壳体理论和基于正交各向异性理论的环肋圆柱壳自由振动方程为基础,运用波传播法得出耦合系统的频率方程,并求出对应外压下的固有频率,通过最小二乘法线性拟合获得临界载荷、外压及固有频率的关系表达式。绘图得知任意边界的同一模态下,水下环肋圆柱壳的固有频率平方与静水压力成正比。结构失稳时,刚度丧失,固有频率降为零,据此求出水下环肋圆柱壳的最小弹性临界载荷。分析了不同边界条件对环肋圆柱壳临界载荷的影响,得知边界约束越强,环肋圆柱壳的临界载荷越大。结果对比表明了本方法的正确性,为水下环肋圆柱壳的固有频率及临界载荷的理论计算提供了一种新的方法。     

Postbuckling of Imperfect Stiffened Cylindrical Shells Under Combined External Liquid Pressure and Axial Compression

- posthuckling analysis is presented for the stilTened cylindrical shell of finite length subjected to combined loading of external liquid pressure and axial compression. The formulations are based on a boundary layer theory of shell buckling, which includes the effects of nonlinear prebuckling deformations, nonlinear large deflections in the postbuckling range and initial geometrical imperfections of the shell. The "smeared stifl'cner" approach is adopted for the stiffencrs. In the analysis a singular perturbation technique is used (o determine the interactive buckling loads and the postbuckling paths. Numerical examples cover the performance of perfect and imperfect, stringer and ring stiffened cylindrical shells. Typical results arc presented in the dimcnsionless graphical form.     

A re‐investigation of the low Reynolds number uniform flow past a porous spherical shell

This work reinvestigates the flow field of a uniform flow past a porous spherical shell based on Song and Huang's (2000) theory of laminar poroelastic media flow with proper boundary conditions. The analytical solution of this study not only indicates viscous effects inside the porous spherical shell, but it also preserves the continuities of tangential velocity and shear stress at the interfaces. The result reveals that as the porosity approaches unity, the flow is entirely comprised of the incident stream; as the porosity approaches zero, the well‐known Stokes' solution of a uniform flow past a sphere with low Reynolds number is obtained. Copyright © 2004 John Wiley & Sons, Ltd.     

球壳形磁性层磁异常正反演方法

为了适应卫星磁测资料解释的需要,作者在磁性界面和磁性层磁异常正反演方法研究的基础上,首次提出了球壳形磁性层磁异常正反演理论和方法。 作者首先阐述了垂直磁化球壳型磁性层模型,导出了场垂直分量Z_(λο)的球谐系数正演公式。然后,给出了磁性层上,下层面埋深变化值与磁化强度变化值的球谐系数反演公式。进而导出了迭代反演收敛条件。本文的磁化强度反演方法不同于目前国内外已有的方法,其反演对象是磁化强度变化值△J_(λθ),磁化强度平均值J是必须给定的定解条件。这种反演方法能给出尽可能满足人们解释要求的解,克服了前人反演方法总存在负值的困难。 为了增加球壳形磁性层反演方法的实用性,作者还在类似平面频率城反演方法所作假设的前提下,把这种利用球谐分析理论在全球面导出的方法,严格地推广应用于局部区域。这也是球谐分析理论在局部区域应用的一个实例,在理沦上具有一定的意义。     

Influence of shotcrete composition on load‐level estimation in NATM‐tunnel shells: Micromechanics‐based sensitivity analyses

Displacement measurement‐based estimations of loads and utilization degrees in shotcrete tunnel shells as part of the New Austrian Tunneling Method (NATM), have become standard tools in tunnel practice; their quality, however, may crucially depend on the knowledge of the actual shotcrete composition after spraying. To shed light on this issue, we here determine, based on experimentally validated micromechanical representations of shotcrete, the hydration degree‐dependent elastic, creep, and strength properties of different shotcretes, characterized by water cement ratios (w/c) between 0.4 and 0.6, aggregate cement ratios (a/c) between 3.5 and 5, and Young's modulus of aggregates (E_agg) between 40 and 80 GPa. These properties are fed into a structural shell model of the Sieberg tunnel, and this model is subjected to displacement fields approximated from daily displacement measurements at five selected points along the shell's inner surface. Resulting stresses and forces in the tunnel shell allow for analyzing the influence of shotcrete composition on load‐level estimation in NATM tunnel shells: The magnitudes of circumferential and longitudinal normal forces increase significantly with decreasing w/c, while a/c and E_agg have the inverse and relatively minor effect. The utilization degree is virtually insensitive to changes in w/c(especially at early ages), and only slightly decreases with decreasing a/c and E_agg. The location of maximum loading is unaffected by the shotcrete composition underlying the analysis. Conclusively, location and magnitude of maximum utilization degrees are very robust estimates (not affected by limited knowledge on the shotcrete composition), whereas realistic estimation of stresses and forces does require more accurate consideration of shotcrete composition. Copyright © 2011 John Wiley & Sons, Ltd.     

皱纹盘鲍(Haliotis discus hannai)不同群体贝壳形态和相对生长变异研究

皱纹盘鲍是我国重要的渔业资源,其地理群体也是我国重要的鲍种质资源来源。不同地理群体间形态及相对生长的差异对皱纹盘鲍资源管理和种质资源鉴定等提供重要参考。以我国自然海区采集的7个皱纹盘鲍群体作为研究材料,通过测定9个形态测量指标并定义了这些测量指标间的17个比率变量,分析了群体内和群体间贝壳形态和体重相对生长的变异。除个别变量外,协方差分析揭示形态比率变量在群体间存在显著变异;异速生长研究揭示了壳形态特征与皱纹盘鲍群体中异速生长差异之间的高度线性相关性,异速生长程度呈现一定的群体区别。非度量多维尺度分析(NMDS)和马氏距离聚类分析(CLUSTER)将皱纹盘鲍群体分为2个组,线性判别分析(LDA)与NMDS和CLUSTER结果相符,然而LDA未能较好的将各种群样本分类。研究结果初步表明皱纹盘鲍表现出与我国沿海环境和/或生态条件差异相关的表型可塑性,然而这种表型差异也可能受到人工底播苗种的遗传影响。研究结果将对于皱纹盘鲍渔业资源管理如管理单位的界定,以及为今后全面评价皱纹盘鲍不同地理群体的遗传变异等提供参考。     

海产经济贝类壳色多态性的研究进展

海产贝类的贝壳颜色是一个对生产、育种有重要意义的可遗传性状.滨螺、海湾扇贝、皱纹盘鲍、牡蛎、蛤类及珍珠贝等贝类的壳色多态性的遗传研究及壳色选择育种的角度综述了目前海产贝类壳色性状遗传机制的研究进展及壳色多态性在育种中的应用,为进一步开展壳色遗传机制和选择育种研究提供理论基础.     

烃源岩对致密油分布的控制作用——以四川盆地大安寨为例

为了研究烃源岩在致密油富集中的意义,本文以有机质含量相对较低、但油气产量较高的四川盆地大安寨为例,通过地球化学分析和理论计算等手段,探讨了烃源岩在致密油生成、排驱和运移等方面的作用。岩心系统热解分析表明,大安寨烃源岩主要集中在中部大一三亚段,有机质含量较高,而上下灰岩段中的泥岩夹层有机质含量则偏低。油岩对比证实,石油主要来自大一三亚段TOC1%的暗色泥岩,说明石油生成后,经历了一定程度的运移和聚集过程,以垂向运移为主,运移方向既可以向上,也可以向下。大安寨工业油井均位于TOC1.2%、生油强度大于10万t/km2范围内,主要油田则主要位于TOC1.4%、生油强度大于20万t/km2范围内。生烃增压既是主要的排烃动力,也是油气充注的主要动力;要形成工业性油气聚集,烃源岩的局部富集非常关键,TOC1%、连续厚度达到20m的烃源岩,可为大安寨致密油富集提供充足的油气来源。