Evidence for microbiological manganese oxidation in the river Tamar Estuary, South West England

Water samples from the Tamar Estuary oxidized manganese when supplemented with Mn²⁺ (2 mgl⁻¹). The rates of oxidation were depressed in the presence of various metabolic inhibitors. The effect of Mn²⁺ and temperature on the rate of manganese oxidation suggested that a biological process was largely responsible for converting Mn²⁺ to Mn⁴⁺. Rates of manganese oxidation were much higher in freshwater (3·32 μgl⁻¹ h⁻¹ in water containing 30 mgl⁻¹ of suspended matter) than in saline water (0·7 μgl⁻¹ h⁻¹ in water of salinity 32‰) containing the same amount of particulate matter. The rate of manganese oxidation was proportional to the particulate load (up to 100 mgl⁻¹ particulates).     

下三叠统鲕粒灰岩中微生物矿化组构特征及意义 ——以川东北地区飞仙关组为例

PTB全球最大生物灭绝事件后,早三叠世被认为是微生物发育繁殖的黄金时期,浅水环境是微生物活动及微生物岩形成的最有利环境。借助高分辨率扫描电镜和能谱分析,在川东北地区开江—梁平海槽西侧下三叠统飞仙关组浅水碳酸盐岩台地边缘的鲕粒灰岩中发现了三种微生物矿化组构:空腔状微生物矿化组构、显微瘤状微生物矿化组构和纤状微生物矿化组构;通过沉积学、岩石学及地球化学的综合分析,认为显微瘤状微生物矿化组构是微生物新陈代谢时形成的纳米球状云质颗粒,纤状微生物矿化组构是微生物的胞外聚合物,而空腔状微生物矿化组构则属于微生物个体。这些微生物矿化组构的发现,为早三叠世微生物大爆发提供了一定的直观证据;推测大量的微生物活动是早三叠世碳酸盐快速沉积现象的直接诱因,并导致于二叠纪末发育的克拉通内裂陷——开江—梁平海槽在早三叠世被快速填平。     

沙漠微生物矿化覆膜及其稳定性的现场试验研究

将微生物诱导矿化技术应用于原位沙漠覆膜的形成,使得流动沙丘经结皮固定而成为半固定、固定沙丘,从根本上阻断沙尘暴的源头。在内蒙古乌兰布和沙漠腹地选择两个微生物矿化试验区域（TP1和TP3）,分别用于两种不同矿化菌种诱导生成碳酸钙覆膜。研究沙漠微生物矿化覆膜的现场试验方法及工艺,对原位矿化覆膜的强度及其在沙漠环境中的长期稳定性进行跟踪检测。采用沙漠土中自行提取的葡萄球菌和传统的巴氏芽孢杆菌两种不同的微生物矿化菌种,通过现场贯入试验检测7、14、28、60、210 d后矿化覆膜沿深度发展的贯入阻力,并将覆膜厚度为2 cm处的平均贯入阻力换算成覆膜层强度,总结覆膜强度随时间的发展变化规律。现场观测结果显示,不同微生物菌种诱导生成的矿化覆膜均在试验的第4天开始形成,到第7天覆膜层具有稳定的强度和厚度,现场检测覆膜的平均厚度为2.0～2.5 cm,经自源葡萄球菌诱导生成的矿化覆膜（TP1）的强度是巴氏芽孢杆菌诱导生成的矿化覆膜（TP3）强度的1.05倍。当经历冬春交替后覆膜层强度都有不同程度的降低,明显地TP3较TP1区域表面剥落更为严重,第210天检测TP3的平均厚度为0.7～1.0 cm,覆膜强度较第7天时降低19%,覆膜内碳酸钙含量较第7天检测时降低15%～30%。而TP1在第210天时的强度较第7天时强度降低仅2%。因此,微生物诱导矿化技术可以应用于沙漠原位覆膜的形成,且沙漠自源葡萄球菌经诱导生成的矿化覆膜层具有更好的强度表现和稳定性。     

微生物诱导碳酸盐沉积改善裂隙岩石防渗性能和强度的试验研究

微生物诱导碳酸盐沉积(MICP)技术主要利用微生物生命活动与环境反应形成的碳酸盐来修复岩土体。为了研究该技术改善含裂隙岩石防渗性能和强度的效果,利用巴氏芽孢杆菌开展了裂隙黄砂岩的修复试验,并对修复后的裂隙黄砂岩进行了无侧限抗压、核磁共振和电镜扫描(SEM)等测试,分析了巴氏芽孢杆菌对裂隙黄砂岩的修复效果和修复机制。研究表明:巴氏芽孢杆菌对裂隙黄砂岩具有较好的修复效果;修复时间越长,巴氏芽孢杆菌的修复效果越好。修复42d后,裂隙黄砂岩的孔隙率下降36.41%,防渗性能提升94.62%,抗压强度增加30.52%。巴氏芽孢杆菌具有较好修复效果原因在于,其诱导产生的碳酸钙能够胶结填充物与试样,大幅降低试样的孔隙率,改善其内部孔隙结构的均质性。     

Deposition and early diagenesis of microbial mud in the Florida Everglades

Microbial carbonate mud in the modern (<4 kyr) palustrine environment of the Florida Everglades is surprisingly susceptible to early diagenetic alteration. The low‐Mg calcite crystals show an open, trellis‐like crystal structure that yields a high surface area to volume ratio. This textural complexity, likely to be a result of organic influence, leads to abundant reaction sites vulnerable to both dissolution and subsequent precipitation. Rapid degradation of organics is attributed to both aerobic and anaerobic metabolisms. Geochemical signatures suggest increased denitrification within the freshwater environment and increased sulphate reduction at the (slightly) brackish mangrove transition zone. A transition from a freshwater to brackish depositional environment is likely to follow the Holocene sea‐level transgression. The textural complexity in these microbial carbonates causes an unexpectedly low preservation potential of original textural and geochemical signatures, even in low‐Mg calcite. Given the potential for early diagenesis of palustrine and perhaps other microbial carbonates, they should be used cautiously as archives for palaeoenvironmental proxies.     

祁连山疏勒河源区冻土退化对土壤微生物生物量碳氮的影响

对青藏高原东北缘祁连山西段疏勒河源区多年冻土区0~50 cm土壤微生物生物量碳氮分布特征及其影响因素进行分析。结果表明:稳定型和极不稳定型多年冻土区0~50 cm土壤中微生物量碳含量范围分别为0.015~0.620 g/kg和0.019~0.411 g/kg,微生物量氮含量范围分别为0.644~12.770 mg/kg和0.207~3.725 mg/kg;土壤微生物量总体呈现出稳定型显著高于极不稳定型多年冻土,表明多年冻土退化（多年冻土由稳定型退化为极不稳定型）对土壤微生物量积累有明显抑制作用。土壤微生物生物量碳占有机碳、微生物生物量氮占全氮的比值在稳定型多年冻土中显著高于极不稳定型,表明多年冻土退化对土壤微生物的矿化能力有明显抑制作用。土壤微生物量及其与土壤养分的比值有显著的剖面变化特征,随土壤深度增加而减小。土壤微生物量碳氮均与土壤温度显著负相关,与地下生物量显著正相关。稳定型多年冻土中,土壤微生物量碳氮与碳氮比正相关、与氧化还原电位负相关;不稳定型多年冻土中,土壤微生物量碳氮与pH正相关。土壤微生物量碳氮与土壤温度和pH在剖面变化上显著相关。逐步回归分析表明驱动微生物生物量碳氮在不同多年冻土类型和土层之间变化的因子是不同的。     

荒漠土壤微生物群落结构特征研究进展

荒漠生态系统占地球陆地面积三分之一,是地球化学循环中的重要部分。荒漠干旱高温、缺乏植被、UV辐射强,曾被认为是没有生命的地方。然而在这恶劣环境中却蕴含有大量的微生物资源,尤其是荒漠土壤富集了大量微生物。微生物参与和主导整个荒漠生态系统地球生物化学循环,对于调节重要生态过程、修复和稳定荒漠生态系统起到重要作用,对其的研究生态学意义突出。本文综述了国内外对荒漠土壤微生物群落结构特征、群落功能多样性以及微生物群落、微生物与植物、微生物与环境之间相互关系的研究现状,旨在充分了解荒漠土壤微生物多样性研究,总结对荒漠微生物生态认识的不足,为荒漠微生物生态研究方向提供参考。     

Enhanced Biodegradation of High-Salinity and Low- Temperature Crude-Oil Wastewater by Immobilized Crude-Oil Biodegrading Microbiota

High salt and low temperature are the bottlenecks for the remove of oil contaminants by enriched crude-oil degrading microbiota in Liaohe Estuarine Wetland(LEW),China.To improve the performance of crude-oil removal,microbiota was further immobilized by two methods,i.e.,sodium alginate(SA),and polyvinyl alcohol and sodium alginate(PVA+SA).Results showed that the crude oil was effectively removed by the enrichment with an average degrading ratio of 19.42-31.45 mg(L d)^?1.The optimal inoculum size for the n-alkanes removal was 10%and 99.89%.Some members of genera Acinetobacter,Actinophytocola,Aquabac-terium,Dysgonomonas,Frigidibacter,Sphingobium,Serpens,and Pseudomonas dominated in crude-oil degrading microflora.Though the removal efficiency was lower than free bacteria when the temperature was 15℃,SA and PVA+SA immobilization im-proved the resistance to salinity.The composite crude-oil degrading microbiota in this study demonstrated a perspective potential for crude oil removal from surface water under high salinity and low temperature conditions.     

Dynamics of Microbiological Contamination at a Marine Recreational Site

Levels of bacterial indicators of pollution are related with marine salinity and turbidity at both high tide (HT) and low tide (LT). The salinity varied from values around 26.9 ppm at the LT and 28.6 ppm at the high tide but affected total and faecal coliform (FC) estimates. Salinity readings of 25–30 ppm produced microbial counts below 10⁻² MPN/100 ml total coliforms (TCs) whereas salinity of 15–22 ppm produced a TC level of 4.6×10⁻⁴ MPN/100 ml. Turbidity peaks in the samples are accompanied by peaks of microbial contamination of the seawater indicating that the contamination is normally deposited at the marine sediment rather than in the water column. In fact, samples collected under heavy stormy weather, in which the water agitation resulted in turbidity values up to 68.3 NTU, produced maximum microbial counts.     

中国南极长城站室内空气微生物含量七年前后的比较

中国南极长城站室内空气微生物含量的考察结果表明,七年后,虽然一些室内空气微生物含量有所下降,即空气质量有所改善,但总体来看,略是上升势。与友邻科考站相比,长城站的多少有点偏高。也比非南极的较高纬度的某些室内空气微生物含量高。结果意味着长城站尚有提高空气质量、改善环境状况的余地。